Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (9): 4153-4160.doi: 10.11843/j.issn.0366-6964.2024.09.039

• Basic Veterinary Medicine • Previous Articles     Next Articles

Intervention of Essential Oils from Citrus reticulata Blanco on Lipopolysaccharide- induced Inflammation in RAW264.7 Cells

Ling LIU1(), Wanyu SHI1, Xiumei LI2, Minghua WANG1, Xianghe ZHAI1,*(), Weiwei ZHOU2,*()   

  1. 1. College of Chinese Veterinary Medicine, Hebei Agricultural University, Baoding 071000, China
    2. Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2023-11-08 Online:2024-09-23 Published:2024-09-27
  • Contact: Xianghe ZHAI, Weiwei ZHOU E-mail:18630616370@163.com;zxh958@163.com;zhouweiwei@caas.cn

Abstract:

The anti-inflammatory effect of essential oils from Citrus reticulata Blanco was investigated based on lipopolysaccharide (LPS)-induced RAW264.7 cell model. The level of Nitric oxide (NO) secretion was detected by Griess method, and the mRNA expression levels of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), interleukin-1β (IL-1β), inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) were determined by real-time PCR method. The results showed that essential oils from Citrus reticulata Blanco significantly decreased the level of NO released from LPS-induced RAW264.7 cells and inhibited the expression of iNOS, COX-2, IL-1β, IL-6 and TNF-α mRNA. In conclusion, essential oils from Citrus reticulata Blanco can inhibit the secretion and expression of inflammatory factors, and thus alleviate the inflammatory response induced by LPS in RAW264.7 cells effectively.

Key words: Citrus reticulata Blanco, essential oils, anti-inflammatory, LPS, RAW264.7 cell

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