Abstract:

Canine mammary gland tumors are a prevalent neoplasm in dogs, with around 50% of cases exhibiting malignancy. They have a conspicuous inclination to spread to other parts of the body and have the potential to readily result in the demise of animals. When the PREX1 protein in the Rho-GTPase family is overstimulated, it can make Rac-GEF less effective, which can mess up the cell cycle and change how cells work. Alterations in PREX1 can potentially facilitate the development of cancerous cells. This study aimed to investigate the influence of PREX1 on the physiological functions of canine mammary tumor cells. In this study, shRNA was used to silence the expression of PREX1. A Western blot test was then used to see how the expression of PREX1 protein changed in the cells after the suppression. The CCK-8 test and colony formation test were used to see how the substance affected the ability of the CHMp canine mammary carcinoma cell line to divide. Additionally, the scratch test and Transwell test were utilized to examine the influence of silencing the substance on the invasiveness of the cell line. Research indicates that reduced expression of PREX1 can impede the proliferation and invasion capacity of CHMp cell lines.

Key words: canine mammary tumor, CHMp, proliferation, migration, invasion