鸭短喙与侏儒综合征病毒分离与鉴定

doi:10.11843/j.issn.0366-6964.2024.08.033

Abstract

Abstract:

A disease characterized by short beak, long tongue, and poor growth was observed in a cherry valley duck farm in Shandong, suspected to be infected with short beak and dwarfism syndrome virus (SBDSV). This study aimed to isolate and identify the virus, and to test its pathogenicity to cherry valley ducks. First, the infection of short beak and dwarfism syndrome virus was confirmed by clinical symptoms, pathological changes, and PCR detection of the diseased materials. Second, after isolating and culturing the virus in SPF duck embryos, it was observed by electron microscopy and analyzed by homology and phylogenetic tree, and then the EID₅₀ of the virus allantoic fluid was tested. Finally, in the animal reversion test, 2-day-old cherry valley ducks were orally administered with allantoic fluid, and the pathogenic characteristics were observed, as well as the virus distribution was detected by PCR and indirect immunofluorescence assay. The results showed that SPF duck embryos died at 96-120 hours post-inoculation, and virus particles of 20-50 nm in size were observed by electron microscopy. The allantoic fluid had an EID₅₀ of 10^-4.85·0.2 mL^-1. The allantoic fluid was positive for SBDSV by PCR, confirming the successful isolation and cultivation of SBDSV from duck embryos. The main clinical manifestations of this case were short beak and weight loss. The virus was shed continuously for two weeks after infection. The VP2 fragment had a 99.8% similarity with the sequence of duck short beak and dwarfism syndrome virus, indicating that it was caused by the infection of this virus, which was named SBDS-SD strain. In the animal challenge experiment, clinical symptoms similar to those of the natural case, such as short beak, diarrhea and significant weight loss, were observed. This experiment successfully isolated a strain of short beak and dwarfism syndrome virus and verified its pathogenicity to cherry valley ducks, providing a basis for further research on this disease.

Key words: duck short beak and dwarf syndrome, pathogenicity test, virus proliferation culture, electron microscope observation

CLC Number:

S852.659.2

Yudian SUN, Ziyue SONG, Hongliang ZHANG, Zhihua QIN, Hu SHAN, Ruimei YANG. Isolation and ldentification of Duckling Short Beak and Dwarfism Syndrome Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3623-3630.

Figures/Tables 5

Fig. 1

Fig. 2

Table 1

Fig. 3

Fig. 4

References 14

1	陈翠腾, 万春和, 傅秋玲, 等.樱桃谷肉鸭源鹅细小病毒的分离与鉴定[J].中国家禽,2015,37(23):47-49.
	CHEN C T , WAN C H , FU Q L , et al.Isolation and identification of duck goose parvovirus from Cherry Valley[J].China Poultry,2015,37(23):47-49.
2	傅宏庆, 姚志兰, 王永娟, 等.鸭短喙型小鹅瘟病毒SBDS-GPV JS01株的分离鉴定及其对雏鸭致病性的研究[J].中国兽医科学,2020,50(10):1286-1293.
	FU H Q , YAO Z L , WANG Y J , et al.Isolation and identification of duck short beak and dwarfism syndrome-goose parvovirus JS01 strain and evaluation of its pathogenicity in ducklings[J].Chinese Veterinary Science,2020,50(10):1286-1293.
3	郝雪飘, 王璐瑶, 雷白时, 等.致鸭短喙与侏儒综合征的新型鹅细小病毒不同传代毒株基因组分析[J].病毒学报,2022,38(2):394-401.
	HAO X P , WANG L Y , LEI B S , et al.Genomic analysis of different-passage strains of the novel goose parvovirus causing short beak and dwarfism syndrome in ducks[J].Chinese Journal of Virology,2022,38(2):394-401.
4	肖世峰, 程晓霞, 陈仕龙, 等.短喙矮小综合征灭活苗母源抗体消长及其被动保护研究[J].中国预防兽医学报,2018,40(8):743-746.
	XIAO S F , CHENG X X , CHEN S L , et al.Determination of maternal antibody variation against short beak and dwarfism syndrome inactivated vaccine and the protection for ducklings[J].Chinese Journal of Preventive Veterinary Medicine,2018,40(8):743-746.
5	磨美兰, 韦正吉, 韦平, 等.传染性支气管炎病毒的分离及其S1基因高变区Ⅰ的遗传变异性分析[J].养禽与禽病防治,2007,(2):2-5.
	MO M L , WEI Z J , WEI P , et al.Isolation of infectious bronchitis virus and genetic variability analysis of S1 gene hypervariable region I[J].Poultry Husbandry and Disease Control,2007,(2):2-5.
6	刘博, 祖立闯, 魏中锋, 等.鸭源新型鹅细小病毒的分离鉴定与VP1基因序列分析[J].中国家禽,2022,44(12):34-39.
	LIU B , ZU L C , WEI Z F , et al.Isolation and identification of a novel goose parvovirus from duck and sequence analysis on VP1 gene[J].China Poultry,2022,44(12):34-39.
7	刘铭, 闫祖杰, 蒲桂婷, 等.抗新型鸭细小病毒卵黄抗体的制备、纯化及效价测定[J].黑龙江畜牧兽医,2020,(10):121-124.
	LIU M , YAN Z J , PU G T , et al.Preparation, purification and titer determination of anti-novel duck parvovirus yolk antibody[J].Heilongjiang Animal Science and Veterinary Medicine,2020,(10):121-124.
8	张锦玥. 新型鹅细小病毒SD15株的分离鉴定及其增殖特性的研究[D]. 成都: 四川农业大学, 2019.
	ZHANG J Y. Isolation, identification and replication characteristic of novel goose parvovirus SD15 strain[D]. Chengdu: Sichuan Agricultural University, 2019. (in Chinese)
9	俞博, 朱小丽, 程晓霞, 等.应用间接免疫荧光法诊断鸭短嘴矮小综合征[J].福建农业学报,2017,32(12):1332-1334.
	YU B , ZHU X L , CHENG X X , et al.Diagnosis of duckling short beak and dwarfism syndrome by indirect immunofluorescence assay[J].Fujian Journal of Agricultural Sciences,2017,32(12):1332-1334.
10	LI P , LIN S , ZHANG R , et al.Isolation and characterization of novel goose parvovirus-related virus reveal the evolution of waterfowl parvovirus[J].Transbound Emerg Dis,2018,65(2):e284-e295.
11	BIAN G Z , MA H B , LUO M P , et al.Identification and genomic analysis of two novel duck-origin GPV-related parvovirus in China[J].BMC Vet Res,2019,15(1):88.
12	XIAO S F , WANG S , JIANG D D , et al.VP2 virus-like particles elicit protective immunity against duckling short beak and dwarfism syndrome in ducks[J].Transbound Emerg Dis,2022,69(2):570-578.
13	张舍郁. IFA检测石蜡和冰冻切片中鸭病毒性肿头出血症病毒方法建立及在检测病毒鸭体内侵染规律的应用[D]. 成都: 四川农业大学, 2007.
	ZHANG S Y. Establishment of indirect immunofluorescence assay for the detection of DSHDV in paraffin-embedded tissues & frozen section and application in detecting the infection characteristics of virus in ducks[D]. Chengdu: Sichuan Agricultural University, 2007.
14	陈少莺, 程晓霞, 陈仕龙, 等.半番鸭新型小鹅瘟病毒病研究简报[J].福建农业科技,2015,46(7):23-25.
	CHEN S Y , CHENG X X , CHEN S L , et al.A new emerging disease in mule ducks caused by a novel goose parvovirus parvovirus[J].Fujian Agricultural Science and Technology,2015,46(7):23-25.

组别 Group	时间 Time	攻毒组 Attack group	对照组 Control group
体重/g	第14天Day 14	147.22±44.05^*	215.00±23.20
Body weight	第21天Day 21	179.77±48.00^**	280.25±21.12
	第35天Day 35	277.14±60.61^**	413.66±20.22
喙长/mm	第14天Day 14	23.15±1.75^*	25.39±0.86
Beak length	第21天Day 21	25.06±2.85^*	28.39±0.88
	第35天Day 35	28.71±3.64^*	33.82±1.05

[1]	Ning ZHOU, Cheng TANG, Jia XU, Hua YUE, Xi CHEN. Pathogenicity and Genomic Characteristics of Feline Panleukopenia Virus A91S Variant in Cats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2560-2568.
[2]	HAN Yang, GUAN Shuaiyin, LI Zhen, ZHOU Saisai, YUAN Honggen, SONG Yunfeng. Prokaryotic Expression and Protein Activity of Porcine Circovirus Type 3 Rep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2061-2071.
[3]	SONG Xiaoqing, DENG Ruide, LI Xin, LI Jiao, LI Runcheng, DU Lifei, DONG Wei, GE Meng. Establishment of ELISA for Detection of PCV4-Cap Antibody and Sero-epidemiological Survey [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2072-2079.
[4]	ZHANG Baoge, HUANG Yaqin, CAI Jinshuang, ZHU Chenguang, LI Yufeng. Preparation of Monoclonal Antibodies to Porcine Circovirus Type 3 Cap Protein and the Establishment of a Blocking ELISA Assay [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1170-1178.
[5]	FAN Jinquan, ZHANG Yuhang, TANG Wuyang, ZHAO Xinyu, LI Pishun, ZHENG Xiaofeng. Inhibitory Effect of Decitabine on Porcine Circovirus Type 2 in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5134-5142.
[6]	SHANG Jinyuan, YAN Manping, YE Jingfei, CHENG Yuening, WANG Zhenjun, FENG Erkai, WANG Chunxia, ZHAO Yan, ZHU Xianpeng, LIAO Yuanjun, LUO Guoliang. Research Progress on Virus Infection and Its Anti-tumor Mechanisms of Protoparvovirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4551-4559.
[7]	LI Chang, ZHOU Ping, WEN Ping, YANG Keli, LIU Wei, GUO Rui, LIU Zewen, YUAN Fangyan, GAO Ting, SONG Haofei, TIAN Yongxiang, ZHOU Danna. Investigation of the Genome-wide Sequence and Immunogenicity of Capsid Protein in Mice for a Porcine Circovirus 2d Genotype Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4289-4299.
[8]	KU Xugang, YU Xuexiang, SUN Qi, LI Panpan, ZHANG Mengjia, LUO Rui, QIAN Ping, HE Qigai. Isolation and Pathogenicity Analysis of Porcine Circovirus Type 3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1568-1578.
[9]	JIA Hanxiao, YUAN Honggen, LI Zhen, GUAN Shuaiyin, ZHANG Jinhua, SONG Yunfeng. Screening of Proteins Interacting with PCV2 Replication Initiation Region and Study on the Regulation of Virus Replication by PARP1 Protein [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3511-3521.
[10]	CHENG Baoyu, LI Zihe, CUI Yanlei, LI Jiahui, YANG Xinwei, YU Yongle, YANG Haiyan, SHAN Hu, ZHANG Chuanmei. Genetic Evolution of Feline Parvovirus and Pathogenicity of an Isolated Strains [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3121-3131.
[11]	HE Qing, LI Zhoumian, WANG Huan, LI Dantong, TAN Qinghui, ZOU Yawen, SU Jianming, DENG Zhibang, YANG Yi, WANG Naidong. Detection and Genetic Evolution of Porcine Circovirus Type 3 in Swine Aborted Fetus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1527-1535.
[12]	ZHOU Qun, YANG Yuan, SONG Xin, HE Xinyi, CAO Hui, ZHANG Bin. Detection of Feline Parvovirus and Mutation Analysis of VP2 Gene in Chengdu from 2017 to 2021 [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1303-1309.
[13]	WANG Mingrui, WU Junhua, WANG Fei, SHAO Hongxia, QIAN Kun, YE Jianqiang, QIN Aijian. Development and Epitope Identification of a Monoclonal Antibody against VP2 Protein of Chicken Infectious Anemia Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 597-606.
[14]	LI Shaohan, YOU Xinyue, FAN Junwen, XU Yi, HAO Yunfeng, CUI Shangjin, QIN Tong. Sequence Analysis of the Complete VP2 and NS1 Genes of 14 Canine Parvovirus Isolates in Beijing [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 262-267.
[15]	WANG Shubo, XU Yigang, CHEN Qiuyan, MEI Zhuyuan, CUI Wen, JIANG Yanping, ZHOU Han, WANG Li, QIAO Xinyuan, LI Yijing, TANG Lijie. Analysis of Immune Response Induced by Recombinant Lactobacillus reuteri Expressing Cap Protein of Porcine Circovirus Type 2 in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2238-2249.

Isolation and ldentification of Duckling Short Beak and Dwarfism Syndrome Virus

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 5

References 14

Related Articles 15

Recommended Articles

Metrics

Comments