艾美耳球虫的遗传操作：平台建立、应用与展望

doi:10.11843/j.issn.0366-6964.2024.08.010

Abstract

Abstract:

The genus of Eimeria parasite is the most common parasite in poultry and livestock, causing severe digestive system diseases in animals such as chickens, rabbits, sheep, and cattle, which affect animal health and the economic benefits of animal farming. With the rapid development of molecular biology technologies, researchers have established genetic manipulation platform for Eimeria, including transient transfection, stable selection, and gene editing. These techniques have realized gene overexpression, gene knockout, and gene editing in Eimeria, greatly advancing the understanding of Eimeria's basic biology and its potential as a novel eukaryotic vector vaccine. However, challenges still remain in this field. This article systematically reviews and summarizes the key technologies, innovative designs, technical bottlenecks, and application prospects of Eimeria's genetic manipulation platform, providing references for the establishment of genetic manipulation platform for other livestock parasites and offering new insights for the development of new control measures against coccidiosis.

Key words: Eimeria spp, genetic manipulation, gene editing, technical bottleneck, vaccine vector

CLC Number:

S852.723

Ruiying LIANG, Jingxia SUO, Lin LIANG, Xianyong LIU, Jiabo DING, Xun SUO, Xinming TANG. Genetic Manipulation of Eimeria: Platform Development, Application, and Perspective[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3362-3373.

Figures/Tables 2

Fig. 1

Table 1

Transient and stable transfection of Eimeria in different recipient cell types"

受体细胞类型 Recipient Cell	虫种名称 Eimeria spp	宿主 Host	报告基因 Reporter	接种方式 Inoculation Routes	瞬时表达 Transient Expression	稳定表达 Stable Expression	参考文献
子孢子 Sporozoite	柔嫩艾美耳球虫 E. tenella	鸡 Chicken	β-半乳糖苷酶 β-galactosidase	—	+	—	[37]
		鸡 Chicken	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	泄殖腔 Cloacal inoculation	+	+	[10, 29, 32]
	和缓艾美耳球虫 E. mitis	鸡 Chicken	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	泄殖腔 Cloacal inoculation	+	+	[14, 39]
	堆型艾美耳球虫 E. acervulina	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	翅下静脉 Intravenous inoculation	+	+	[19, 40]
	大型艾美耳球虫 E. magna	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	手术接种 Surgical inoculation	+	+	[15]
	肠艾美耳球虫 E. intestinalis	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	手术接种 Surgical inoculation	+	+	[16]
	中型艾美耳球虫 E. media	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	手术接种 Surgical inoculation	—	+	未发表数据
	斯氏艾美耳球虫 E. stiedai	小鼠 Mouse	荧光蛋白 Fluorescent Protein	静脉接种 Intravenous inoculation	—	+	未发表数据
	镰型艾美耳球虫 E. falciformis	大鼠 Rat	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	经口接种 Oral inoculation	—	+	[18, 41]
	尼氏艾美耳球虫 E. nieschulzi		荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	经口接种 Oral inoculation	+	+	[30]
裂殖子 Merozoite	毒害艾美耳球虫 E. necatrix	鸡 Chicken	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	泄殖腔 Cloacal inoculation	—	+	[13, 42]
孢子囊 Sporocyst	镰型艾美耳球虫 E. falciformis	小鼠 Mouse	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	经口接种 Oral inoculation	—	+	未发表数据
卵囊 Oocyst	巨型艾美耳球虫 E. maxima	鸡 Chicken	荧光蛋白 Fluorescent Protein	—	—	—	[43]

Table 1

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Genetic Manipulation of Eimeria: Platform Development, Application, and Perspective

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