单碱基水平上胞嘧啶碱基编辑器(CBE)的研究进展

doi:10.11843/j.issn.0366-6964.2020.01.001

Abstract

Abstract: Although the new generation of gene editing technology CRISPR/Cas9 has many advantages, it is often inefficient when performing mutations at a single base level. Due to the uncertainties in DNA double-strand breaks, homology directed repair (HDR) based on donor template occurs only in the actively dividing cells, however, non-homologous end joining (NHEJ) works in the whole cell cycle, therefore, it seems to be insufficient when traditional CRISPR/Cas9 performs gene editing at a single base level. The advent of the base editor (BE) has greatly relieved this dilemma. The cytosine base editor (CBE) or the adenine base editor (ABE) can both achieve C·G to T·A or A·T to G·C conversion without causing double-strand breaks, thus greatly improving the application value of single base editing. This paper focuses on the principle, development, application and existing problems of CBE, in order to provide useful reference for the application of efficient single base mutation tools in biomedical and animal husbandry production.

Key words: gene editing, single base mutation, cytosine base editor

CLC Number:

LI Guangdong, ZHANG Lu, FU Juncai, LIAN Zhengxing, LIU Guoshi. Research Progress of Cytosine Base Editor at the Single Base Level[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 1-8.

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Research Progress of Cytosine Base Editor at the Single Base Level

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