Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (6): 2727-2740.doi: 10.11843/j.issn.0366-6964.2024.06.043
• Clinical Veterinary Medicine • Previous Articles Next Articles
Xuanyi WANG(), Yawei SUN*(
), Yuwei LONG, Liying WANG, Yuxin ZHOU, Na LI, Xuelian MA, Hongqiong ZHAO, Gang YAO
Received:
2023-08-10
Online:
2024-06-23
Published:
2024-06-28
Contact:
Yawei SUN
E-mail:1020877416@qq.com;sunyawei2008@163.com
CLC Number:
Xuanyi WANG, Yawei SUN, Yuwei LONG, Liying WANG, Yuxin ZHOU, Na LI, Xuelian MA, Hongqiong ZHAO, Gang YAO. Correlation Analysis of FOXP3, FSHR, FMR1 Gene Polymorphisms and Reproductive Hormones in Infertile Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2727-2740.
Table 1
Primer sequences information"
基因 Gene | 登录号 NCBI ID | 引物序列(5′→3′) Primers sequences | 退火温度/℃ Annealing temperature | 产物长度/bp Product length |
FMR1 | 507482 | F: 5′-ACGCGGTTTCACTGTTTACAC-3′ | 49.8 | 310 |
R: 5′-ACCTTGTAGAAAGCGCCATTG-3′ | ||||
FOXP3 | 506053 | F: 5′-ATTCTAAGGTTCTGGCATCGT-3′ | 60.3 | 618 |
R: 5′-GCCAGAGCATATAAGAAACCACT-3′ | ||||
FSHR | 281172 | F: 5′-CTGCCTCCCTCAAGGTGCCCCTC-3′ | 58.0 | 306 |
R: 5′-AGTTCTTGGCTAAATGTCTTAGGGGG |
Table 2
Gene frequency and genotype frequency of different varieties"
品种 Variety | 基因型频率 Gene frequency | 基因频率 Genotype frequency | χ2 | P值 P value | ||||
AA | AG | GG | A | G | ||||
西门塔尔牛Simmental heifer | 0.70 | 0.27 | 0.03 | 0.84 | 0.16 | 0.826 | 0.363 | |
褐牛Brown cattle | 0.62 | 0.30 | 0.08 | 0.77 | 0.23 | 0.241 | 0.623 | |
安格斯牛Aberdeen angus | 0.55 | 0.36 | 0.09 | 0.73 | 0.27 | 5.046 | 0.025* |
Fig. 6
PCR product of bovine FSHR gene was digested by Alu Ⅰ M. DL700 DNA marker; 1, 3, 6 and 8. The produced two enzyme fragments (243 and 63 bp) for genotype CC; 5. genotype CG produced four fragments (243, 193, 63 and 50 bp); 2, 4. The genotype GG produced three enzyme fragments (193, 63 and 50 bp); 7. PCR products of control; N. The control lane without PCR product"
Table 4
The frequency of FSHR genotype in different breeds of cattle and the difference of mutation between the two groups of cattle"
品种 Variety | 基因型频率 Gene frequency | 基因频率 Genotype frequency | χ2 | P值 P value | ||||
AA | AC | CC | A | C | ||||
西门塔尔牛Simmental heifer | 0.52 | 0.35 | 0.13 | 0.70 | 0.30 | 2.267 | 0.132 | |
褐牛Brown cattle | 0.66 | 0.30 | 0.04 | 0.81 | 0.19 | 0.584 | 0.445 | |
安格斯牛Aberdeen angus | 0.75 | 0.21 | 0.04 | 0.86 | 0.14 | 7.198 | 0.007** |
Table 7
Association analysis of FOXP3 gene SNP locus polymorphism and hormone concentration"
激素 Hormone | FOXP3基因型FOXP3 genotype | ||
AA(n=126) | AG(n=61) | GG(n=14) | |
E | 112.84±1.42a | 113.05±1.72b | 107.9±4.05a |
FSH | 35.30±0.48 | 35.46±0.64 | 31.81±0.82 |
PRL | 235.54±3.24 | 235.90±4.82 | 223.26±8.70 |
T | 33.92±0.47 | 34.83±0.49 | 32.72±0.95 |
LH | 3371.76±50.46 | 3451.92±74.14 | 3190.61±104.33 |
Table 8
Association analysis of FSHR gene SNP locus polymorphism and hormone concentration"
激素 Hormone | FSHR基因型FSHR genotype | ||
AA(n=144) | AC(n=70) | CC(n=18) | |
E | 114.01±1.26a | 112.07±2.04a | 99.65±3.92b |
FSH | 35.56±0.47 | 34.41±0.69 | 34.51±0.83 |
PRL | 240.01±3.12 | 228.05±3.87 | 215.41±18.59 |
T | 34.66±0.37 | 33.02±0.76 | 35.04±0.81 |
LH | 3389.64±46.27 | 3364.35±83.89 | 3474.08±167.33 |
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