1株类NADC30与类JXA1重组的猪繁殖与呼吸综合征病毒基因组遗传特征分析

doi:10.11843/j.issn.0366-6964.2020.07.020

Abstract

Abstract: To investigate the genetic characteristics of a porcine reproductive and respiratory syndrome virus (PRRSV) strain isolated in Henan Province in 2017, the complete genome was sequenced and further analyzed by bioinformatics. The results showed that the length of the genome is 15 054 bp, and the whole genome shared 84.5%, 93.4%, 84.2%, 83.9%, and 82.1% nucleotide similarities with VR-2332, NADC30, CH-1a, JXA1, and QYYZ, respectively. Phylogenetic analysis displayed that the isolate was clustered into the same branch with NADC30-like strains (lineage 1). For GP5 protein of the isolated strain, the N glycosylation sites were identified at 34, 44, and 51 sites. The linear epitope (37-45 aa) was conserved during NADC30-like strains while the other two linear epitopes (27-30 aa, 37-45 aa) have specific amino acid mutations. The Nsp2 of the isolate has 131 discontinuous amino acid deletion(323-433,483,504-522)which was a genetic marker of NADC30-like strains. Furthermore, the results of recombination analysis showed that the isolate was a mosaic virus between the major parent strain NADC30-like and minor parent strain JXA1-like. The recombinant region was in Nsp2 (1 340-2 082 nt). Our studies described the genetic characteristics of a NADC30-like strain and confirmed the recombinant events that happened in the Nsp2 region. It suggests that PRRSV undergoes genetic evolution through recombination and it is important to continuous monitoring of PRRSV prevalence and genetic variation.

Key words: porcine reproductive and respiratory syndrome virus, homological analysis, phylogenetic tree, genetic variation, gene recombination

CLC Number:

GUO Zhenhua, RUAN Haiyu, GENG Rui, CHEN Xinxin, QIAO Songlin, DENG Ruiguang, ZHANG Gaiping. Genetic Characteristics of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus between NADC30-like and JXA1-like[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1677-1687.

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Genetic Characteristics of a Recombinant Porcine Reproductive and Respiratory Syndrome Virus between NADC30-like and JXA1-like

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