杂交组合断奶仔猪腹泻与FUT1基因遗传变异的关联分析

doi:10.11843/j.issn.0366-6964.2021.03.005

Abstract

Abstract: The study aimed to explore the relationship between diarrhea of weaned piglets of hybrid combination and genetic variation of α-1,2-fucosyltransferase 1 (FUT1) gene. In this experiment, 35-day-old Dudian, Duzang and Duroc weaned piglets were selected as the research objects, and were divided into 3 groups according to breeds, with 113, 165 and 87 replicates, respectively. FUT1 gene polymorphism was detected by sequencing to analyze the correlation between gene polymorphism and diarrhea rate of weaned piglets. The results showed that there were 4 polymorphic sites in FUT1 gene, including T18C, C229T, C2418A and A306G, all of which were in moderate polymorphism and heterozygosity. C229T, C2418A and A306G were missense mutations, which might be key sites affecting porcine diarrhea. The degree of diarrhea was relatively serious in the hybrid combination Dudian, Duzang pig with TT genotype at T18C, and 3 pig populations with CC genotype at C229T, CA genotype at C2418A and GG genotype at A306G, which showed dominant inheritance. The result suggest that the effects of breeds and FUT1 gene polymorphisms on weaned piglets diarrhea have certain differences and correlations.

Key words: hybrid combination, FUT1 gene, genetic variation, weaned piglet, diarrhea, disease resistance breeding

CLC Number:

S828.2

FANG Chen, GUO Fei, HU Ruiju, YANG Minghua, ZHANG Bin, LIU Shaona, HUANG Ying, ZHAO Yanguang, ZHAO Sumei. Analysis of Correlation between Diarrhea and Genetic Variation of FUT1 Gene in Weaned Piglets of Hybrid Combination[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 610-619.

References 40

[1]	BOLDIN B.Persistence and spread of gastro-intestinal infections:the case of enterotoxigenic Escherichia coli in piglets[J].Bull Math Biol,2008,70(7):2077-2101.
[2]	HESSELAGER M O,EVEREST-DASS A V,THAYSEN-ANDERSEN M,et al.FUT1 genetic variants impact protein glycosylation of porcine intestinal mucosa[J].Glycobiology,2016,26(6):607-622.
[3]	ZI C,WU Z C,WANG J,et al.Transcriptional activity of the FUT1 gene promoter region in pigs[J].Int J Mol Sci,2013,14(12):24126-24134.
[4]	BERTIN A M,DUCHET-SUCHAUX M F.Relationship between virulence and adherence of various enterotoxigenic Escherichia coli strains to isolated intestinal epithelial cells from Chinese Meishan and European large white pigs[J].Am J Vet Res,1991,52(1):45-49.
[5]	BAO W B,YE L,PAN Z Y,et al.The effect of mutation at M307 in FUT1 gene on susceptibility of Escherichia coli F18 and gene expression in Sutai piglets[J].Mol Biol Rep,2012,39(3):3131-3136.
[6]	LUISE D,MOTTA V,BERTOCCHI M,et al.Effect of Mucine 4 and Fucosyltransferase 1 genetic variants on gut homoeostasis of growing healthy pigs[J].J Anim Physiol Anim Nutr (Berl),2019,103(3):801-812.
[7]	KIM K,NGUYEN D T,CHOI M,et al.Alpha (1,2)-fucosyltransferase M307A polymorphism improves piglet survival[J]. Anim Biotechnol,2013,24(3):243-250.
[8]	PETIT G,GROSBOIS V,CHALVET-MONFRAY K,et al.Polymorphism of the alpha-1-fucosyltransferase (FUT1) gene in several wild boar (Sus scrofa) populations in France and link to edema disease[J].Res Vet Sci,2020,131:78-86.
[9]	孙鹏翔,吴圣龙,包文斌,等.苏太猪FUT1基因多态性及其与生产性能的关联分析[J].中国畜牧杂志,2007,43(5):4-6.SUN P X,WU S L,BAO W B,et al.Polymorphism of FUT1 gene and its relationship with performances in Sutai pigs[J]. Chinese Journal of Animal Science,2007,43(5):4-6.(in Chinese)
[10]	张引红,周忠孝,曹果清.FUT1基因多态性及其与产仔性状的关联性研究[J].遗传,2007,29(1):52-56.ZHANG Y H,ZHOU Z X,CAO G Q.FUT1 gene polymorphism and its association with litter size in pigs[J].Hereditas,2007, 29(1):52-56.(in Chinese)
[11]	张新银,陈家丰,赵志超,等.FUT1基因对大长杂交母猪产仔数的影响[J].畜牧与饲料科学,2010,31(10):111-112.ZHANG X Y,CHEN J F,ZHAO Z C,et al.Effect of FUT1 gene on litter size of sows with hybridization between Yorkshire and landrace[J].Animal Husbandry and Feed Science,2010,31(10):111-112.(in Chinese)
[12]	叶兰,潘章源,黄雪根,等.苏太猪抗F18+大肠杆菌病基础群FUT1基因多态性及其与生长发育的关联分析[J].中国畜牧兽医,2010,37(4):158-161.YE L,PAN Z Y,HUANG X G,et al.Polymorphism of FUT1 relationship with growth and development in foundation colony of Sutai ETEC F18+ resistant breeding pigs[J].China Animal Husbandry and Veterinary Medicine,2010,37(4):158-161.(in Chinese)
[13]	何小平,彭勇波,樊斌,等.猪α-1岩藻糖转移酶基因多态性检测及与性状关联的研究[J].养猪,2006(3):26-28.HE X P,PENG Y B,FAN B,et al.Detection of polymorph polymorphism of fucosyltransferase gene in porcine -1 and its Correlation with its characters[J].Swine Production,2006(3):26-28.(in Chinese)
[14]	吴晓东.致断奶仔猪腹泻和水肿病大肠杆菌F18菌毛单克隆抗体的研究及其初步应用[D].扬州:扬州大学,2001.WU X D.Development and potential application of monoclonal antibodies against F18 pili of Escherichia coli from swine with postweaning diarrhea and edema disease[D].Yangzhou:Yangzhou University,2001.(in Chinese)
[15]	熊邓.猪杂种优势的利用[J].养殖与饲料,2002(6):13-17.XIONG D.Utilization of pig heterosis[J].Culture and Feed,2002(6):13-17.(in Chinese)
[16]	陶泽宇,薛盼盼,张潇筠,等.猪杂交改良研究进展[J].家畜生态学报,2019,40(4):81-84.TAO Z Y,XUE P P,ZHANG X J,et al.Research progress on the technology of swine crossbreeding[J].Acta Ecologae Animalis Domastici,2019,40(4):81-84.(in Chinese)
[17]	鲁绍雄,连林生.滇南小耳猪种质资源研究进展与开发利用[J].中国猪业,2013,8(S1):165-167.LU S X,LIAN L S.Research progress and development and utilization of small ear pig germplasm resources in southern Yunnan province[J].China Swine Industry,2013,8(S1):165-167.(in Chinese)
[18]	严达伟.迪庆藏猪种质特性研究[C]//中国畜牧业协会,海口市人民政府.2006中国猪业发展大会论文集.海口:中国畜牧业协会,2006:207-213.YAN D W.Studies on the germplasm characteristics of Diqing Tibetan pig[C]//China Animal Husbandry Association,Haikou Municipal People's Government.2006 China Pig Industry Development Conference Proceedings.Haikou:China Animal Husbandry Association,2006:207-213.(in Chinese)
[19]	魏红艳.商品猪杂种优势的利用[J].农村实用科技信息,2006(7):22.WEI H Y.Utilization of Heterosis in commercial pigs[J].Rural Practical Science and Technology Information,2006(7):22.(in Chinese)
[20]	杨岸奇,陈斌,冉茂良,等.基因组选择在猪杂交育种中的应用[J].遗传,2020,42(2):145-152.YANG A Q,CHEN B,RAN M L,et al.The application of genomic selection in pig cross breeding[J].Hereditas, 2020, 42(2):145-152.(in Chinese)
[21]	李涛,黄涛,胡九英,等.大白猪NCOA1和FUT1基因多态性与繁殖性能的关联分析[J].中国畜牧杂志,2017,53(6):58-63.LI T,HUANG T,HU J Y,et al.Association analysis of NCOA1 and FUT1 gene polymorphisms and reproductive performance in large white pigs[J].Chinese Journal of Animal Husbandry,2017,53(6):58-63.(in Chinese)
[22]	BAO W B,YE L,ZHU J,et al.Evaluation of M307 of FUT1 gene as a genetic marker for disease resistance breeding of Sutai pigs[J].Mol Biol Rep,2012,39(4):4223-4228.
[23]	李聪聪,刘思雨,吴姣,等.不同品种猪SLA-DQA、FUT1和MUC4基因遗传变异初析[J].黑龙江畜牧兽医,2020(2):55-61.LI C C,LIU S Y,WU J,et al.Genetic variation of SLA-DQA,FUT1 and MUC4 genes in pigs of different varieties[J].Heilongjiang Animal Science and Veterinary Medicine,2020(2):55-61.(in Chinese)
[24]	罗艳茹.仔猪大肠杆菌F18粘附型与FUT1基因型相关性研究[D].兰州:甘肃农业大学,2007.LUO Y R.Analysis of correlation between adhesion phenotype of Escherichia coli F18 and genotype of FUT1 gene on porcine[D].Lanzhou:Gansu Agricultural University,2007.(in Chinese)
[25]	朱弘焱,赵颂,赵微,等.东北荷包猪FUT1基因多态性及其与产仔性能的关联分析[J].安徽农业科学,2010, 38(1):88-90.ZHU H Y,ZHAO S,ZHAO W,et al.Polymorphism of FUT1 gene and its relationship with litter size in northeast Hebao pigs[J].Journal of Anhui Agricultural Sciences,2010,38(1):88-90.(in Chinese)
[26]	RⅡS POULSEN A S,LUISE D,CURTASU M V,et al.Effects of alpha-(1,2)-fucosyltransferase genotype variants on plasma metabolome,immune responses and gastrointestinal bacterial enumeration of pigs pre- and post-weaning[J].PLoS One,2018,13(8):e0202970.
[27]	ZHANG Y,WANG M,YU X Q,et al.Analysis of polymorphisms in the FUT1 and TAP1 genes and their influence on immune performance in Pudong White pigs[J].Genet Mol Res,2015,14(4):17193-17203.
[28]	DONG W H,DAI C H,SUN L,et al.Expression of key glycosphingolipid biosynthesis-globo series pathway genes in Escherichia coli F18-resistant and Escherichia coli F18-sensitive piglets[J].Anim Genet,2016,47(4):428-435.
[29]	杜永新,项勋,段纲,等.FUT1基因对猪生产性能的影响分析[J].中兽医医药杂志,2017,36(5):32-35.DU Y X,XIANG X,DUAN G,et al.Influence of FUT1 gene on pig production performance[J].Journal of Traditional Chinese Veterinary Medicine,2017,36(5):32-35.(in Chinese)
[30]	包文斌,叶兰,潘章源,等.苏太猪FUT1基因M307位点多态性及其对部分免疫指标的遗传效应分析[J].畜牧兽医学报,2010,41(10):1219-1224.BAO W B,YE L,PAN Z Y,et al.Polymorphism and genetic effect of FUT1 gene M307 on some immune parameters in Sutai pigs[J].Acta Veterinaria et Zootechnica Sinica,2010,41(10):1219-1224.(in Chinese)
[31]	吴圣龙,原志伟,鞠慧萍,等.苏太仔猪FUT1基因M307位点多态性与F18大肠杆菌抗病相关性的体外鉴定[J].中国预防兽医学报,2007,29(10):783-787.WU S L,YUAN Z W,JU H P,et al.Polymorphisms of the FUT1 gene M307 locus in post-weaning Sutai breed piglet and resistance to F18 fimbrial Escherichia coli in vitro[J].Chinese Journal of Preventive Veterinary Medicine,2007,29(10):783-787.(in Chinese)
[32]	吴圣龙,包文斌,鞠慧萍,等.猪a1-岩藻糖转移酶基因(FUT1)M857位点遗传变异分析[J].遗传,2007,29(9):1071-1076.WU S L,BAO W B,JU H P,et al.Analysis of genetic variations at M857 locus of the a1-Fucosy-transferase (FUT1) ORF in pigs[J].Hereditas,2007,29(9):1071-1076.(in Chinese)
[33]	周利华,舒青龙,彭秋玲,等.FUT1基因新cSNPs的鉴别及其对仔猪抗大肠杆菌F18侵染的影响[J].中国农业科学,2011,44(8):1720-1726.ZHOU L H,SHU Q L,PENG Q L,et al.A novel coding variant in the FUT1 gene and its effects on the resistance to Escherichia coli F18 infection in piglets[J].Scientia Agricultura Sinica,2011,44(8):1720-1726.(in Chinese)
[34]	XIA R W,QIN W Y,GAN L N,et al.Differential expression of FUT1 and FUT2 in Large White,Meishan,and Sutai porcine breeds[J].Genet Mol Res,2016,15(1):15017613.
[35]	LEMUS-FLORES C,MEJIA-MARTINEZ K,RODRIGUEZ-CARPENA J G,et al.Genetic diversity and variation of ESR,RBP4 and FUT1 genes in Mexican creole and Yorkshire pig populations[J].J Biol Sci,2009,9(8):878-883.
[36]	YAN X M,REN J,GUO Y M,et al.Research on the genetic variations of a1-fucosytransferase (FUT1) gene in 26 pig breeds[J].Acta Genet Sinica,2003,30(9):830-834.
[37]	FALKOVÁ L,VRTKOVÁ I,KRATOCHVÍLOVÁ L.Boar SNP variability in genetic resource přeštice black-pied pig[J].Res Pig Breed,2014,8:4-7.
[38]	CIOBANU D C,DAY A E,NAGY A,et al.Genetic variation in two conserved local Romanian pig breeds using type 1 DNA markers[J].Genet Sel Evol,2001,33(4):417-432.
[39]	VRTKOVÁ I,MATOUŠEK V,STEHLÍK L,et al.Genomic markers important for health and reproductive traits in pigs[J].Res Pig Breed,2007,1:4-6
[40]	BAO W B,WU S L,MUSA H H,et al.Genetic variation at the alpha-1-fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and Western commercial pig breeds[J].J Anim Breed Genet,2008,125(6):427-430.

Analysis of Correlation between Diarrhea and Genetic Variation of FUT1 Gene in Weaned Piglets of Hybrid Combination

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