基于16S rDNA测序分析腹腔注射苦参碱的昆明小鼠肠道菌群结构

doi:10.11843/j.issn.0366-6964.2022.02.028

Abstract

Abstract: The study aimed to analyse the structure of the intestinal flora of Kunming mice injected with matrine by 16S rDNA sequencing. Twenty Kunming mice were randomly divided into 2 groups named as matrine group (MT group) and negative control group (NC group). The intraperitoneal administration was administered twice a day for 5 days, the feces and intestinal tissues of each group were collected for β diversity, Lefse and Metastats analysis. qPCR was used to detect the mRNA expression of different strains in each intestinal segment, and KEGG was used to analyze the differences in metabolic pathways caused by the changes in intestinal flora. The rarefaction curve results showed that the measured sample data was sufficient to reflect the species diversity in the samples. The results of β diversity analysis showed that matrine regulated the structure of the intestinal flora. Lefse and Metastats analysis results showed that matrine significantly increased the abundance of Bacteroidetes, Bacteroidales, Muribaculaceae and probiotic Lactobacillus acidophilus, and significantly reduced the abundance of Firmicutes, Ruminococcaceae and Desulfovibrio. The qPCR results showed that the expression of Lactobacillus acidophilus in the feces of mice in the matrine group was increased, which was consistent with the results of Lefse and Metastats analysis. It was also found that matrine enhanced the colonization of Lactobacillus acidophilus in various segments of the intestine. KEGG analysis found that there were significant differences in metabolic pathways such as glycan biosynthesis and metabolism, transport and catabolism between NC and MT groups. The study results showed that matrine significantly changed the structure of the intestinal flora in mice, increase the colonization of probiotic Lactobacillus acidophilus in the intestine and caused the differences in metabolic pathways such as glycan biosynthesis and metabolism, transport and catabolism. These results have accumulated data for further study of the mechanism of matrine exerting its pharmacological effects.

Key words: matrine, Kunming mice, intestinal flora, 16S rDNA sequencing, Lactobacillus acidophilus

CLC Number:

S852.6

CAO Zhigang, WANG Hong, ZHANG Hua, SUN Panpan, LI Hongquan, SUN Yaogui, YANG Huizhen, WANG Jianzhong, YIN Wei, FAN Kuohai, SUN Na. Influence of Matrine on Intestinal Flora of Kunming Mice through Intraperitoneal Injection Based on 16S rDNA Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 618-627.

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Influence of Matrine on Intestinal Flora of Kunming Mice through Intraperitoneal Injection Based on 16S rDNA Sequencing

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