IFN-α对山羊副流感病毒3型的抗病毒活性

doi:10.11843/j.issn.0366-6964.2023.02.030

Abstract

Abstract: The aim of this study was to investigate the antiviral activity of goat interferon α (IFN-α) to caprine parainfluenza virus 3 (CPIV3). The sequence characteristics of goat IFN-α was analyzed, and the homology of IFN-α from different species was compared. Then the recombinant expression plasmid pET-30a-gIFN-α by inserting goat IFN-α gene (removing the signal peptide gene sequence) into vector pET-30a was constructed and transformed into Rosetta (DE3), after induced by IPTG, the goat IFN-α was expressed and purified. Then the relative expression level of interferon-stimulated genes (ISGs) was measured, and the anti-CPIV3 activity was measured after exogenous goat IFN-α treatment on Madin-Darby bovine kidney cell(MDBK cell) by TCID₅₀ and Western blot. The results showed that, the protein concentration of goat IFN-α was 0.20 mg·mL^-1, and Western blot analysis showed that the relative molecular weight was about 20 ku, which was consistent with expectations. The RT-qPCR result showed that the incubation of goat IFN-α on MDBK cells induced the up-regulation of the six ISGs including RSAD2, STAT1 and ISG15. Meanwhile, the goat IFN-α inhibited the CPIV3 replication, and down-regulated the expression level of the viral nonstructural protein C. All above results indicated that goat IFN-α could significantly inhibit the proliferation of CPIV3 on MDBK cells. This study extended the research type I interferon, and promoted the prevention and control of ruminant diseases.

Key words: goat IFN-α, prokaryotic expression, ISGs, caprine parainfluenza virus 3, antiviral activity

CLC Number:

S858.27

SUN Min, HAO Fei, ZHANG Wenwen, LI Wenliang, YANG Leilei, MAO Li, CHENG Zilong, LIU Maojun. The Antiviral Activity of Goat Interferon Alpha to Caprine Parainfluenza Virus 3[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 736-743.

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The Antiviral Activity of Goat Interferon Alpha to Caprine Parainfluenza Virus 3

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