藏猪对饲粮纤维的消化及其与肠道微生物的相关性研究

doi:10.11843/j.issn.0366-6964.2022.09.022

Abstract

Abstract: With the aim of exploring the potential factors of strong fibre degradation ability for Tibetan pigs, this experiment was conducted to study the digestion of dietary cellulose and hemicellulose and their correlation with the faecal bacteria of Tibetan pigs. The apparent digestibility of dietary fibre to grazing Tibetan pigs, captive Tibetan pigs and commercial pigs[Duroc×Landrace×Yorkshire (DLY) pigs] at 150 days of age was determined by digestion test. Faecal samples were collected, and the full-length sequence of the 16S rRNA gene of faecal bacteria was determined by single molecule real-time sequencing technology. The structure and diversity of faecal bacterial community were analysed. Spearman correlation analysis was used to determine the correlation between apparent digestibility of dietary fibre and faecal bacterial community. Results showed that the apparent digestibility of dietary cellulose and hemicellulose of grazing Tibetan pigs was significantly higher than that of captive Tibetan pigs and DLY pigs (P<0.05). A total of 15 phyla, 26 classes, 48 orders, 87 families, 190 genera and 419 species of bacteria were identified from faeces of grazing Tibetan pigs, captive Tibetan pigs and DLY pigs. Furthermore, there were 1 phylum (Fibrobacteres), 3 genera (Alloprevotella, Fibrobacter, and Succinivibrio) and 3 species (Alloprevotella rava, Fibrobacter intestinalis, and Succinivibrio dextrinosolvens) which relative abundance in grazing Tibetan pigs were significantly higher than those of captive Tibetan pigs and DLY pigs (P<0.05), which were significantly positively correlated with apparent digestibility of dietary fibre (P<0.05). In conclusion, grazing Tibetan pigs have strong ability of fibre digestion, which was closely related to the fibre-degrading bacteria in faeces.

Key words: Tibetan pigs, ability of fibre digestion, bacteria, correlation

CLC Number:

TAN Zhankun, SHANG Zhenda, CHU Guiyan, CAI Chuanjiang, ZHU Yanbin, DENG Wen, QIANGBA Yangzong, LIU Suozhu. Study on Dietary Fibre Digestion and Its Correlation with Intestinal Microbiome of Tibetan Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3063-3078.

References 43

[1]	YANG Y N, YUAN H N, YANG Q L, et al.Post-transcriptional regulation through alternative splicing in the lungs of Tibetan pigs under hypoxia[J].Gene, 2022, 819:146268.
[2]	谭占坤, 商振达, 刘锁珠, 等.西藏高原藏猪盲肠微生物群落结构与多样性的研究[J].畜牧兽医学报, 2020, 51(9):2147-2155.TAN Z K, SHANG Z D, LIU S Z, et al.Study on the cecal microbial community structure and diversity of Tibetan pigs in Tibetan plateau[J].Acta Veterinaria et Zootechnica Sinica, 2020, 51(9):2147-2155.(in Chinese)
[3]	谭占坤, 池福敏, 商振达, 等.西藏高原放牧藏猪结肠微生物的特异性研究[J].饲料研究, 2021, 44(8):68-72.TAN Z K, CHI F M, SHANG Z D, et al.Colonic microbial specificity of grazing Tibetan pigs on Tibetan plateau[J].Feed Research, 2021, 44(8):68-72.(in Chinese)
[4]	MENG F, MA L, JI S, et al.Isolation and characterization of Bacillus subtilis strain BY-3, a thermophilic and efficient cellulase-producing bacterium on untreated plant biomass[J].Lett Appl Microbiol, 2014, 59(3):306-312.
[5]	谭碧娥, 伍树松, 贺建华, 等.地方猪耐粗饲和肉质性状形成的微生物代谢机制[J].动物营养学报, 2020, 32(7):2941-2946.TAN B E, WU S S, HE J H, et al.Metabolic mechanism of coarse feeding tolerance and meat quality traits formation based on microbial metabolism in local pigs[J].Chinese Journal of Animal Nutrition, 2020, 32(7):2941-2946.(in Chinese)
[6]	谭占坤, 刘瑶, 刘锁珠, 等.藏猪肠道因子与饲粮粗纤维表观消化率的相关性研究[J].高原农业, 2021, 5(5):465-471.TAN Z K, LIU Y, LIU S Z, et al.Study on the correlation between intestinal factors and apparent digestibility of dietary crude fiber in Tibetan pigs[J].Journal of Plateau Agriculture, 2021, 5(5):465-471.(in Chinese)
[7]	谭占坤, 池福敏, 商振达, 等.放牧藏猪、舍饲藏猪与商品猪粪便真菌群落组成及其与饲粮纤维消化的相关性研究[J].微生物学报, 2022, 62(1):259-274.TAN Z K, CHI F M, SHANG Z D, et al.Fungal community in the feces of grazing Tibetan pigs, captive Tibetan pigs, and commercial pigs and its interaction with dietary fiber digestion[J].Acta Microbiologica Sinica, 2022, 62(1):259-274.(in Chinese)
[8]	中华人民共和国农业部.NY/T 65-2004 猪饲养标准[S].北京:中国农业出版社, 2004.Ministry of Agriculture of the People's Republic of China.NY/T 65-2004 Feeding standard of swine[S].Beijing:China Agriculture Press, 2004.(in Chinese)
[9]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 20806-2006 饲料中中性洗涤纤维(NDF)的测定[S].北京:中国标准出版社, 2007.General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.GB/T 20806-2006 Determination of neutral detergent fiber in feedstuffs[S].Beijing:Standards Press of China, 2007.(in Chinese)
[10]	中华人民共和国农业部.NY/T 1459-2007 饲料中酸性洗涤纤维的测定[S].北京:农业出版社, 2008.Ministry of Agriculture of the People's Republic of China.NY/T 1459-2007 Determination of acid detergent fiber in feedstuff (ADF)[S].Beijing:China Agriculture Press, 2008.(in Chinese)
[11]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 20805-2006 饲料中酸性洗涤木质素(ADL)的测定[S].北京:中国标准出版社, 2007.General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.GB/T 20805-2006 Determination of acid detergent lignin in feedstuffs[S].Beijing:Standards Press of China, 2007.(in Chinese)
[12]	中华人民共和国国家质量监督检验检疫总局, 中国国家标准化管理委员会.GB/T 23742-2009 饲料中盐酸不溶灰分的测定[S].北京:中国标准出版社, 2009.General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China.GB/T 23742-2009 Animal feeding stuffs-Determination of ash insoluble in hydrochloric acid[S].Beijing:Standards Press of China, 2009.(in Chinese)
[13]	CALLAHAN B J, MCMURDIE P J, ROSEN M J, et al.DADA2:high-resolution sample inference from Illumina amplicon data[J].Nat Methods, 2016, 13(7):581-583.
[14]	ROGNES T, FLOURI T, NICHOLS B, et al.VSEARCH:A versatile open source tool for metagenomics[J].PeerJ, 2016, 4:e2584.
[15]	BOLYEN E, RIDEOUT J R, DILLON M R, et al.Reproducible, interactive, scalable and extensible microbiome data science using QIIME 2[J].Nat Biotechnol, 2019, 37(8):852-857.
[16]	KŌLJALG U, NILSSON R H, ABARENKOV K, et al.Towards a unified paradigm for sequence-based identification of fungi[J].Mol Ecol, 2013, 22(21):5271-5277.
[17]	彭健.日粮纤维:定义、成分、分析方法及加工影响(综述)[J].国外畜牧学(猪与禽), 1999(4):8-11.PENG J.Dietary Fibre:definition, composition, analytical methods and processing effects (review)[J].Animal Science Abroad(Pigs and Poultry), 1999(4):8-11.(in Chinese)
[18]	许振英.猪与饲料纤维[J].养猪, 1990(3):37-43.XU Z Y.Pig and feed fiber[J].Swine Production, 1990(3):37-43.(in Chinese)
[19]	BURKEY T E, SKJOLAAS K A, MINTON J E.BOARD-INVITED review:porcine mucosal immunity of the gastrointestinal tract[J].J Anim Sci, 2009, 87(4):1493-1501.
[20]	杨海天.日粮纤维水平对不同遗传基础育肥猪生长性能及肉品质的影响[D].长春:吉林农业大学, 2019.YANG H T.Effects of dietary fiber levels on growth performance and meat quality of different genetically based finishing pigs[D].Changchun:Jilin Agricultural University, 2019.(in Chinese)
[21]	KEMP B, DEN HARTOG L A, KLOK J J, et al.The digestibility of nutrients, energy and nitrogen in the Meishan and Dutch Landrace pig[J].J Anim Physiol Anim Nutr, 1991, 65(1-5):263-266.
[22]	徐孝义.东北民猪与哈白猪日粮养分消化性的比较研究(摘要)[J].黑龙江畜牧兽医, 1985(12):33-34.XU X Y.Comparative study on nutrient digestibility of diets between northeast Min-pigs and Harbin white pigs (Abstract)[J].Heilongjiang Animal Science and Veterinary Medicine, 1985(12):33-34.(in Chinese)
[23]	牛清.基于16S rRNA基因测序技术鉴别与猪纤维消化相关的肠道微生物[D].南京:南京农业大学, 2019.NIU Q.Identification of pig gut microbiota for fiber digestibility by 16S rRNA gene sequencing[D].Nanjing:Nanjing Agricultural University, 2019.(in Chinese)
[24]	YEN J T, VAREL V H, NIENABER J A.Metabolic and microbial responses in western crossbred and Meishan growing pigs fed a high-fiber diet[J].J Anim Sci, 2004, 82(6):1740-1755.
[25]	LYND L R, WYMAN C E, GERNGROSS T U.Biocommodity engineering[J].Biotechnol Prog, 1999, 15(5):777-793.
[26]	JENKINSON D S, ADAMS D E, WILD A.Model estimates of CO2 emissions from soil in response to global warming[J].Nature, 1991, 351(6324):304-306.
[27]	WILSON D B.Three microbial strategies for plant cell wall degradation[J].Ann N Y Acad Sci, 2008, 1125(1):289-297.
[28]	BAYER E A, BELAICH J P, SHOHAM Y, et al.The cellulosomes:multienzyme machines for degradation of plant cell wall polysaccharides[J].Ann Rev Microbiol, 2004, 58:521-554.
[29]	RANSOM-JONES E, JONES D L, MCCARTHY A J, et al.The Fibrobacteres:an important phylum of cellulose-degrading bacteria[J].Microb Ecol, 2012, 63(2):267-281.
[30]	AMANN R I, LIN C Z, KEY R, et al.Diversity among Fibrobacter isolates:towards a phylogenetic classification[J].Syst Appl Microbiol, 1992, 15(1):23-31.
[31]	DOWNES J, DEWHIRST F E, TANNER A C R, et al.Description of Alloprevotella rava gen.nov., sp. nov., isolated from the human oral cavity, and reclassification of Prevotella tannerae Moore et al.1994 as Alloprevotella tannerae gen. nov., comb. nov[J].Int J Syst Evol Microbiol, 2013, 63(Pt 4):1214-1218.
[32]	肖晓蓉, 李燕, 肖丽英.2009-2012年发现的人口腔细菌新种属简介[J].华西口腔医学杂志, 2013, 31(2):217-220.XIAO X R, LI Y, XIAO L Y.The novel species and genus discovered and nominated from the human oral cavity in 2009-2012[J].West China Journal of Stomatology, 2013, 31(2):217-220.(in Chinese)
[33]	张君胜, 徐盼, 陶勇, 等.不同生长性能苏姜猪保育猪肠道菌群差异分析[J].微生物学通报, 2020, 47(12):4240-4249.ZHANG J S, XU P, TAO Y, et al.Analysis on the difference of intestinal microflora of Sujiang piglets with different growth performance[J].Microbiology China, 2020, 47(12):4240-4249.(in Chinese)
[34]	任敏敏, 杨华, 项云, 等.饲粮纤维水平对金华猪生长性能、盲肠菌群结构和短链脂肪酸含量的影响[J].动物营养学报, 2020, 32(6):2575-2585.REN M M, YANG H, XIANG Y, et al.Effects of dietary fiber levels on growth performance, microbial community structure and short-chain fatty acid content in cecum of Jinhua pigs[J].Chinese Journal of Animal Nutrition, 2020, 32(6):2575-2585.(in Chinese)
[35]	唐诗.宿主基因型与肠道微生物互作对猪生长和脂肪沉积性状的影响机制研究[D].武汉:华中农业大学, 2021.TANG S.Interaction between host genetic variants and gut microbiota and its effect on growth and fat deposition traits in pigs[D].Wuhan:Huazhong Agricultural University, 2021.(in Chinese)
[36]	刘阳.妊娠后期高可溶或不可溶纤维摄入对母猪繁殖性能和围产期免疫功能及肠道菌群的影响[D].雅安:四川农业大学, 2019.LIU Y.Effects of dietary soluble or insoluble fiber intake in late gestation on reproductive performance, immune function and intestinal microbiota of sows around parturition[D].Ya'an:Sichuan Agricultural University, 2019.(in Chinese)
[37]	王庆昭, 赵学明.琥珀酸发酵菌种研究进展[J].生物工程学报, 2007, 23(4):570-576.WANG Q Z, ZHAO X M.The research progress of succinic acid fermentation strains[J].Chinese Journal of Biotechnology, 2007, 23(4):570-576.(in Chinese)
[38]	YU T, WANG Y, CHEN S C, et al.Low-molecular-weight chitosan supplementation increases the population of Prevotella in the cecal contents of weanling pigs[J].Front Microbiol, 2017, 8:2182.
[39]	XU R Y, LU Y, WANG J, et al.Effects of the different dietary fibers on luminal microbiota composition and mucosal gene expression in pig colons[J].J Funct Foods, 2019, 59:71-79.
[40]	WANG X F, TSAI T, WEI X Y, et al.Effect of lactylate and Bacillus subtilis on growth performance, peripheral blood cell profile, and gut microbiota of nursery Pigs[J].Microorganisms, 2021, 9(4):803.
[41]	LYU Z Q, WANG L, WANG J R, et al.Oat bran and wheat bran impact net energy by shaping microbial communities and fermentation products in pigs fed diets with or without xylanase[J].J Anim Sci Biotechnol, 2020, 11(1):99.
[42]	杨伟平.藏猪肠道细菌群落组成与纤维素分解菌的研究[D].杨凌:西北农林科技大学, 2015.YANG W P.A study on the bacteria community and the cellulolytic bacterium in Tibetan pigs[D].Yangling:Northwest A&F University, 2015.(in Chinese)
[43]	DIAO H, YAN H L, XIAO Y, et al.Intestinal microbiota could transfer host Gut characteristics from pigs to mice[J].BMC Microbiol, 2016, 16(1):238.

Study on Dietary Fibre Digestion and Its Correlation with Intestinal Microbiome of Tibetan Pigs

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 43

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Wenli, WANG Haoqi, ZE Licuo, GAO Yufan, ZHANG Feifan, ZHANG Jian, DUAN Mengqi, SHANG Peng, QIANG Bayangzong. Polymorphism of Pro-Inflammatory Factors (IL-1β, IL-6, TNF-α) in Tibetan Pigs and Its Association Analysis with Immune Traits [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1958-1969.
[2]	HUANG Jie, RUAN Zihao, CAI Rui. Advances of the Application of Antimicrobial Peptides in the Preservation of Porcine Semen at Room Temperature [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1401-1411.
[3]	GAO Yuanji, LIU Chang, CHEN Miao, CHEN Songbiao, ZHANG Junfeng, LI Jing, JIA Yanyan, LIAO Chengshui, GUO Rongxian, DING Ke, YU Zuhua, SHANG Ke. Structure, Secretory Characteristics, and Pathogenic Mechanism of Bacterial Outer Membrane Vesicles [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 971-983.
[4]	XIA Shuwen, CHEN Kunlin, SHEN Yangyang, AN Zhenjiang, ZHAO Fang, DING Qiang, ZHONG Jifeng, LIN Zhiping, WANG Huili. The Estimation of Genetic Parameters for Longevity Traits of Holstein Cows in Jiangsu Region [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1030-1039.
[5]	WANG Kang, LIU Geyan, WANG Yu, YANG Zhen, TANG Xinwei, CAO Sanjie, HUANG Xiaobo, YAN Qigui, WU Rui, ZHAO Qin, DU Senyan, WEN Xintian, WEN Yiping. Preparation of Glaesserella parasuis Ghosts Vaccine Delivering Porcine Circovirus Type 2 DNA Vaccine and Evaluation of Its Immunoprotective Effect in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1179-1191.
[6]	FU Xuezhen, LI Xincan, QIAN Hongyu, LÜ Hong, WU Chanyu, WANG Xiaohan, WANG Xiaohua, WANG Zhiying, ZHOU Zuoyong. Antibacterial Effect and Mechanism of Bergamot Essential Oil on Corynebacterium pseudotuberculosis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1217-1227.
[7]	MI Hui, PENG Can, HE Zhixiong, TAN Zhiliang. Separation of Sheep Secretory Immunoglobulin A Coated Bacteria by Flow Cytometry [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2924-2931.
[8]	MAO Peng, WANG Zhihao, LI Jianji, CUI Luying, ZHU Guoqiang, MENG Xia, DONG Junsheng, WANG Heng. Research Progress of Ferroptosis in Bacterial Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2280-2287.
[9]	HONG Bo, SUN Qi, LI Dongfan, YU Xuexiang, KU Xugang, HE Qigai. Investigation of Bacterial Communities in Water Samples from Flood-affected Pig Farms Using 16S rDNA High-throughput Sequencing Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2092-2100.
[10]	SUN Yufan, YU Panyuan, CHEN Hongyu, TAN Yiqing, CHEN Xiabing, ZHANG Tengfei, GAO Ting, ZHOU Rui, LI Lu. Evaluation of the Efficacy of Potassium Diformate in the Prevention of Salmonella Infection and the Effect on Intestinal Flora [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2101-2113.
[11]	BI Yazhen, SHANG Mingyu, HU Wenping, ZHANG Li. Correlation and Regression Analysis among Growth Traits and Association Analysis of TRHDE Gene Polymorphism with Growth Traits in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1415-1428.
[12]	GUO Mingpeng, MENG Yuan, WANG Honghao, WANG Yuan, CHE Leijie, SHEN Li, WANG Xi. Estimation of Genetic Parameters of Body Weight and Body Size Traits in Jinnan Cattle at Different Growth Stages [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1452-1464.
[13]	HAN Yunzhen, YANG Wenpan, HONG Yuan, LONG Yi, LIU Xiangjie, FAN Xiaoping, LI Wenjing, DENG Zheng, LIU Minghui, ZHENG Sumei, RUAN Guorong, DING Nengshui. Estimating the Inbreeding Coefficient of Huai Pigs by Different Methods Based on Chip Data [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 947-955.
[14]	YU Shiqiang, LI Liuxue, ZHAO Xiaobo, ZHAO Huiying, TU Yan, ZHAO Yuchao, JIANG Linshu. Differences and Correlations of Lactation Performance in Chinese Holstein Dairy Cows at Different Lactation Stages and Somatic Levels [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1003-1014.
[15]	WANG Weihao, DUAN Yan, WANG Hongdi, DOU Lu, LIU Ting, KANG Letian, SUN Lina, AO Tehenggerile, JIN Ye. Effects of Feeding Regimens on Growth Performance, Slaughter Performance, Meat Quality and Rumen Bacteria Community of Sunit Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1085-1094.