西门塔尔牛、和牛与荷斯坦牛杂种优势预测及实际杂交效果分析

doi:10.11843/j.issn.0366-6964.2022.08.015

Abstract

Abstract: The aim of the study was to predict heterosis of different crosses for growth and carcass traits by single nucleotide polymorphism (SNP) markers at the whole genome level in Simmental, Wagyu, and Holstein. The Illumina Bovine HD 770 K and GGP Bovine 100 K chips were used to detect genotypes of 1 222 Simmental, 464 Wagyu and 43 Holstein, respectively. The trait-associated SNP markers were obtained by blasting Illumina Bovine HD 770 K and GGP Bovine 100K chips with QTLs database of birth weight, yearling weight, and carcass weight in NCBI. The genetic distance (GD) was used to predict heterosis of different crosses based on genomic SNPs and trait-associated SNP markers. The combining ability was estimated to verify heterosis effect of Simmental and Holstein in practice. The results showed that the genetic distances were no significant difference between genome-wide and trait-associated SNPs in two hybrid groups. At the genome-wide level, the genetic distances of Simmental(♂)×Holstein(♀) and Wagyu(♂)×Holstein (♀) were 0.346 1 and 0.338 9, respectively. For trait-associated SNPs with birth weight, yearling weight and carcass weight, the genetic distances of Simmental(♂)×Holstein(♀) were 0.343 1, 0.348 7 and 0.336 7, it was 0.337 6, 0.340 7 and 0.329 2 in Wagyu(♂)×Holstein (♀), respectively. In short, Simmental(♂)×Holstein(♀) had the larger GD than Wagyu(♂)×Holstein (♀). Therefore, Simmental(♂)×Holstein(♀) would obtain greater heterosis in birth weight, yearling weight and carcass weight. According to combining ability test of German Simmental×Holstein, it was found that general and specific combining ability of 10 German Simmental sires had positive effect on birth weight, and the highest value were 3.760 9, 8.931 2, respectively. Hybridization of Simmental and Holstein would obtain greater heterosis for birth weight, yearling weight, and carcass weight.

Key words: Simmental, Wagyu, Holstein, heterosis prediction, heterotic effect

CLC Number:

S823.2

FAN Tingting, WANG Wenxiang, MA Yi, ZHAO Guoyao, XU Lingyang, CHEN Yan, ZHANG Lupei, GAO Huijiang, LI Junya, GAO Xue. Prediction and Effect Analysis of Heterosis in Simmental, Wagyu and Holstein[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2568-2577.

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Prediction and Effect Analysis of Heterosis in Simmental, Wagyu and Holstein

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