SRSF10对山羊肌内前体脂肪细胞分化的影响

doi:10.11843/j.issn.0366-6964.2022.06.011

Abstract

Abstract: This experiment aimed to clone goat SRSF10 gene sequence, reveal its biological characteristics, and clarify the effect of SRSF10 on goat intramuscular adipocyte differentiation through overexpression and interference techniques. Taking Jianzhou big ear goat(Capra hircus)as the experimental object, the goat SRSF10 gene sequence was cloned by RT-PCR technique, along with bioinformatics analysis. The real-time quantitative PCR(qPCR)technique was used to investigate its expression level in adipogenic differentiation of cells at different stages. pcDNA3.1-SRSF10 overexpression vector and SRSF10-siRNA were transfected into goat intramuscular preadipocytes, followed by inducing differentiation. Oil red O and Bodipy staining were used to clarify the effect of SRSF10 on lipid droplet accumulation, and the expression changes of adipocyte differentiation marker genes were detected by qPCR technique. The results showed that the obtained goat SRSF10 gene sequence was 1 026 bp in length, the CDS region was 552 bp, encoding a total of 183 amino acids. The highest expression level of SRSF10 in goat was detected at 96 h in intramuscular adipocytes after inducing differentiation. Overexpression and interference of goat SRSF10 promoted and inhibited the accumulation of lipid droplets in intramuscular adipocytes, respectively. After overexpression of SRSF10, the relative expression of SREBP1, PPARγ and C/EBPα extremely significantly up-regulated(P < 0.01), and the relative expression of differentiation marker genes SREBP1, PPARγ and C/EBPα extremely significantly down-regu-lated after interference(P < 0.01). The results show that goat SRSF10 is a positive regulatory factor for the differentiation of intramuscular adipocytes, and this regulation may be achieved mainly through regulating the expression of SREBP1, PPARγ and C/EBPα.

Key words: goat, SRSF10, gene cloning, expression characteristics, intramuscular preadipocytes, cell differentiation

CLC Number:

S827.2

LIU Kehan, WANG Yong, LI Yanyan, WANG Chongyang, ZHU Jiangjiang, XIONG Yan, LI An, LIN Yaqiu. Effects of SRSF10 on the Differentiation of Intramuscular Preadipocytes in Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1768-1778.

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Effects of SRSF10 on the Differentiation of Intramuscular Preadipocytes in Goats

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