共轭亚油酸影响边鸡胸肌脂类代谢的相关差异基因的筛选

doi:10.11843/j.issn.0366-6964.2022.05.016

Abstract

Abstract: The study aimed to screen out the genes related to lipid metabolism in breast muscle of Bian chickens affected by conjugated linoleic acids(CLA) through transcriptome sequencing technology, and provide the theoretical basis for the mechanism of dietary CLA regulating the lipid metabolism. In this experiment, 180 90-day-old healthy Bian chickens were randomly divided into 5 gourps with 3 replicates in each group, and chickens in different groups were fed diets added different proportions CLA 0% (control group), 0.5%, 1.0%, 1.5% and 2.0%, respectively, the pre-feeding period lasted for 1 week followed by an experimental period of 6 weeks. After the feeding experiment, breast muscles were collected for transcriptome sequencing. DESeq2 software was used for differential expression analysis of the sequencing data, the differentially expressed genes were detected by GO function and KEGG pathway enrichment analysis, and differentially expressed genes related to lipid metabolism in breast muscle were screened out. the differentially expressed genes were verified by qPCR.Through analyzing transcriptome sequencing data, a total of 1 229 differentially expressed genes were screened out, 594 genes were up-regulated and 635 genes were down-regulated. The results of 9 randomly selected differentially expressed genes by qPCR verification showed that the relative expression trend was consistent with the sequencing results. GO functional analysis found that differentially expressed genes were mainly concentrated in the biological process, including cellular process, single-organism process, biological regulation and metabolic process. KEGG pathway enrichment analysis showed that the differentially expressed genes in each experimental group were significantly enriched in different signaling pathways, mainly enriched in ECM-receptor interaction, focal adhesion, phagosome, apoptosis, regulation of actin cytoskeleton and cytokine-cytokine receptor interaction. Through GO and KEGG function annotation analysis, a total of 18 main differentially expressed genes involved in lipid metabolism were screened out, including MCAT, APOA1, PTGDS, ALDH3A2, PLTP, FABP3, FOXO1, UCP3 and FABP4, etc., which may play an important role in regulating the lipid metabolism in breast muscle of Bian chickens. In this study, transcriptome sequencing was used to screen the main regulatory genes in the process of CLA affecting lipid metabolism in breast muscles of Bian chicken, and the main biological processes and signaling pathways were found, which lays the foundation for the future study of the molecular mechanism of CLA affecting the lipid metabolism in breast muscle of Bian chickens.

Key words: Bian chickens, conjugated linoleic acids, transcriptome, lipid metabolism

CLC Number:

ZHANG Junzhen, ZHANG Meng, LI Yali, CHANG Qiangqiang, SUN Tianyuan. Screening of Differentially Expressed Genes Related to Lipid Metabolism in Breast Muscle of Bian Chickens by Adding Conjugated Linoleic Acids in Diet[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1486-1499.

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Screening of Differentially Expressed Genes Related to Lipid Metabolism in Breast Muscle of Bian Chickens by Adding Conjugated Linoleic Acids in Diet

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