牛种布鲁氏菌Ⅳ型分泌系统对巨噬细胞内质网应激和细胞凋亡的影响

doi:10.11843/j.issn.0366-6964.2022.04.019

Abstract

Abstract: In order to study the effect of Brucella bovis type IV secretion system (T4SS) on the endoplasmic reticulum stress and apoptosis of macrophages, the molecular pathogenic mechanism of Brucella was deeply explored. In this study, bovine Brucella vaccine strain A19 and its T4SS promoter deletion strain A19ΔVirB were used to infect mouse macrophages. RNA and total protein were collected at 6, 12 and 24 hours after infection, transcription level and protein expression level of endoplasmic reticulum stress marker molecules GRP78 and CHOP, apoptosis related molecules BAX and BCL-2 were detected by qRT-PCR and Western blot, apoptosis at different time points was detected by flow cytometry. The results showed that at 6, 12 and 24 h after infection, the transcription level and protein expression level of GRP78 in deletion strain A19ΔVirB group were significantly lower than those in parent A19 group (P<0.05); at 6 h after infection, the transcription level and protein expression level of CHOP in the deletion strain group were also significantly lower than those in the parent strain (P<0.05), but at 24 h, it was significantly higher than that of the parent strain (P<0.05); at 24 h after infection,transcription level and protein expression level of BAX in deletion strain A19ΔVirB group were significantly higher than those in parental strains (P<0.05); at 24 h after infection,transcription level and protein expression level of BCL-2 in deletion strain A19ΔVirB group were significantly lower than those in parental strain (P<0.05); the results of flow cytometry further showed that the apoptosis rate of the deletion strain group was significantly higher than that of the parent strain (P<0.05). The results showed that deletion of T4SS would weaken the ability of Brucella bovis to induce endoplasmic reticulum stress and enhance its ability to induce apoptosis.This study laid a foundation for further understanding the pathogenic mechanism of Brucella, and also provided a scientific basis for the functional research of Brucella T4SS in the future.

Key words: Brucella, deletion strain, T4SS, endoplasmic reticulum stress, apoptosis

CLC Number:

YANG Qin, DENG Xiaoyu, XIE Shanshan, YI Jihai, WANG Yong, ZHANG Qian, WANG Zhen, CHEN Chuangfu. Effects of Brucella bovis Type IV Secretion System on Endoplasmic Reticulum Stress and Apoptosis of Macrophages[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1192-1200.

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Effects of Brucella bovis Type IV Secretion System on Endoplasmic Reticulum Stress and Apoptosis of Macrophages

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