基于转录组测序研究健康三益菌对肉鸡脂质代谢的影响

doi:10.11843/j.issn.0366-6964.2022.04.015

Abstract

Abstract: The study aimed to investigate the main genes and pathways of healthy tribeneficial bacteria regulating lipid metabolism in broilers, and to elucidate the mechanism of their effects on lipid metabolism. A total of 200 one-day-old male Jute broilers were randomly divided into 2 groups with 5 replicates per group and 20 broilers per replicate. The individuals in control group drank water normally, and the individuals in probiotic group drank water added with 1% healthy tribeneficial bacteria compound preparation (Lactobacillus casei∶Lactobacillus acidophilus∶Bifidobacterium=1∶1∶2). The experiment lasted for 42 days. The meat quality of broilers were measured and ileal transcriptome sequencing was performed. The results showed that, compared with the control group, the abdominal fat percentage and shear force of broilers supplemented with healthy tribeneficial bacteria decreased, and the breast muscle percentage and intramuscular fat increased (P>0.05). Transcriptome sequencing showed that there were 12 significantly differentially expressed genes related to lipid metabolism. Compared with the control group, the expressions levels of LPL, PLIN1, GOS2, FABP4, RBP7, THRSP, FABP3, SCD, THEM4 and CYP2C18 in probiotic group were significantly up-regulated (P<0.05), and the expressions levels of CYP7B1 and GGT5 were significantly down-regulated (P<0.05). These genes were mainly enriched in GO items such as response to fatty acids, chylomicron remodeling, response to lipid, white fat cell differentiation and long-chain fatty acid binding, as well as important pathways related to steroid hormone biosynthesis, PPAR signaling pathway, arachidonic acid metabolism and regulation of lipolysis in adipocytes. The results showed that healthy tribeneficial bacteria can affect lipid metabolism, reduce abdominal fat content and promote intramuscular fat deposition of broilers by regulating the related pathways and the expression of genes related to lipid deposition, transport and decomposition.

Key words: healthy tribeneficial bacteria, broilers, lipid metabolism, transcriptome

CLC Number:

S831.4

ZHANG Lihuan, JIA Hao, WANG Yanfei, ZHANG Ruonan, LIU Xuan. Effects of Healthy Tribeneficial Bacteria on Lipid Metabolism in Broilers Based on Transcriptome Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1154-1164.

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Effects of Healthy Tribeneficial Bacteria on Lipid Metabolism in Broilers Based on Transcriptome Sequencing

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