Acta Veterinaria et Zootechnica Sinica ›› 2021, Vol. 52 ›› Issue (8): 2265-2274.doi: 10.11843/j.issn.0366-6964.2021.08.019

• PREVENTIVE VETERINARY MEDICINE • Previous Articles     Next Articles

Effect of Radix Paeoniae Rubra and Cortex Phellodendri Preparation on Intestinal Bacterial Community Diversity of Chickens Artificially Infected with Pathogenic Escherichia coli

GUO Shuang1, CAI Xiaoli2, HU Zongfu1, NIU Huaxin1, MA Dehui1, WANG Zi1, FU Liyun2, CHEN Zhiqiang2*, LIU Kai1*   

  1. 1. College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China;
    2. Inner Mongolia Huatian Pharmaceutical Co. Ltd., Chifeng 024000, China
  • Received:2021-01-28 Online:2021-08-23 Published:2021-08-21

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Abstract: The study aimed to understand the effects of Radix paeoniae rubra and Cortex phellodendri preparation on the intestinal flora diversity of chickens infected with pathogenic Escherichia coli. Two hundred and forty 15-day-old chicks were randomly divided into 4 groups:blank control group (CON), infection group (BC), low-dose treatment group (TL) and high-dose treatment group (TH). Except for the control group, all the treatment groups (BC, TL, TH) were infected with pathogenic Escherichia coli. TL (4 mL·L-1 sterilized water) and TH (12 mL·L-1 sterilized water) groups were treated with different concentration of Radix paeoniae rubra and Cortex phellodendri preparation by drinking water after infection of 0.5 d. After 5 days of treatment, the morbidity and mortality of each group were calculated, and high-throughput sequencing technology was used to detect the diversity of bacterial community in the duodenum of chicks. The results showed that, compared with the BC group, the TH group significantly reduced the morbidity and mortality of chicks (P<0.05). Bacterial community analysis showed that, the preparation processing changed the intestinal bacterial community in the chicks. The dominant bacteria were Firmicutes and Enterococcus in CON group; Firmicutes, Proteobacteria, Enterococcus, Escherichia and Staphylococcus in BC group; Firmicutes, Lactobacillus, Enterococcus and Staphylococcus in TL group; Firmicutes and Lactobacillusin in TH group. The heatmap analysis showed that Lactobacillus was negatively correlated with morbidity and mortality, while Escherichia was positively correlated with morbidity and mortality (P<0.05). In summary, the infection of Escherichia coli and the preparation treatment of Radix paeoniae rubra and Cortex phellodendri changed the diversity and composition of intestinal bacterial community in chicks, and the changes of intestinal bacterial community may have an important impact on the incidence and disease resistance of diarrhea in chicks.

Key words: Radix paeoniae rubra and Cortex phellodendri preparation, chick, pathogenic Escherichia coli, intestinal bacterial community, high throughput sequencing

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