Abstract: Bordetella bronchiseptica and Klebsiella pneumoniae infections are extremely harmful to the breeding of rabbits. They often cause multiple respiratory diseases. To determine the pathogens that cause dyspnea and diarrhea in rabbits, suspected dead rabbits in the rabbit farm were subjected to necropsy. Subsequently, we collected tissues for the isolation and identification of pathogenic bacteria and drug sensitivity tests. The results showed that we obtained a gram-negative, blunt-round, small rod-shaped suspected pathogen and a gram-negative, oval, suspected pathogen; their 16S rRNA gene sequence amplified fragment sizes were 1 492 and 1 494 bp, respectively. The homology of B. bronchiseptica AU 12671 and K. pneumoniae strain F5feb.57 was 99.85% and 100.00%, respectively. The rabbits were identified as B. bronchiseptica and K. pneumoniae mixed infection. The drug susceptibility test found that they are all sensitive to gentamicin, ceftriaxone, and cefazolin, and they have varying degrees of resistance to other drugs. In summary, the bacteria we isolated are Bordetella bronchiseptica and Klebsiella pneumonia. This work provides a reference for the isolation and identification of B. bronchiseptica and K. pneumoniae coinfection and clinical scientific medications during rabbit breeding.

Key words: rabbit, Bordetella bronchiseptica, Klebsiella pneumoniae, respiratory tract disease, diarrhea, tolerance