土曲霉对小鼠肝氧化损伤及铁死亡相关指标的影响

doi:10.11843/j.issn.0366-6964.2021.012.028

Abstract

Abstract: In order to explore the mechanism of Aspergillus terreus causing liver injury of mice, 20 Kunming mice were divided into a control group and a test group. The test group was intraperitoneally injected with 0.3 mL (5×10⁷CFU·mL^-1) spore suspension. The trail was 7 days. The indicators of oxidative damage in mouse liver tissue were evaluated by malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GSH-PX) and superoxide dismutase (T-SOD) kits. The iron content of liver tissue was determined by iron kit. Prepare liver paraffin sections to observed the pathological changes and iron accumulation by HE and Perls stain. The ultrastructure of hepatocytes was observed by transmission electron microscope. The mRNA relative transcription level of ferroptosis-related genes in liver tissue were detected by qPCR. The results were as follows:compared with control group, the content of MDA in test group was significantly increased, but the content of GSH,the activities of GSH-PX and T-SOD in test group were significantly decreased (P<0.01). Meanwhile, the content of iron ions in test group was significantly increased (P<0.01). Some hepatocytes were swollen and necrotic. A few inflammatory cells were infiltrated. Iron ions accumulated in the center of hepatic loules. The mitochondria atrophied, cristae reduced, and membrane density increased in hepatocytes. The relative mRNA transcription level of transferrin receptor protein 1 (TFR1), iron uptake-related proteins divalent metal transporter-1 (DMT1), ferritin heavy chain 1 (FTH1), and voltage-dependent anion channel 3 (VDAC3) genes were significantly up-regulated (P<0.05), while, the relative mRNA transcription level of glutathione peroxidase 4 (GPX4) and solute carrier family-7 member-11 (SLC7A11) genes were significantly down-regulated (P<0.05). These results indicated that Aspergillus terreus MSF2 could cause ferroptosis in the liver of mice. It benefits for further study of the pathogenic mechanism of Aspergillus terreus.

Key words: Aspergillus terreus, liver, oxidative damage, ferroptosis

CLC Number:

S852.3

XIANG Yi, ZHANG Hua, WANG Li, WEI Yong, EMU Quzhe. Effects of Aspergillus terreus on Oxidative Damage and Ferroptosis Related Indicators in Mice Liver[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3619-3626.

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Effects of Aspergillus terreus on Oxidative Damage and Ferroptosis Related Indicators in Mice Liver

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