猪脾转移因子提高LaSota株鸡新城疫弱毒疫苗的免疫保护率

doi:10.11843/j.issn.0366-6964.2021.012.024

Abstract

Abstract: This study was conducted to evaluate the immune enhancement effect and mechanism of pig spleen transfer factor(TF) on Newcastle disease virus(NDV) attenuated vaccine LaSota strain. Specific pathogen free(SPF) chickens were inoculated with different doses(10^-2, 10^-3,10^-4, 10^-5) of NDV strain LaSota(vaccination group without TF), NDV strain LaSota combined with TF(vaccination group with TF), respectively, and challenged with virulent NDV strain F₄₈E₉ on 14 days post vaccination(dpv). The control group(non-immunized but challenged) and blank group(non-immunized and non-challenged) were set up simultaneously. In addition, the change of IL-4, IFN-γ and IL-12 P40 in peripheral blood were analyzed by ELISA and protein chip technology. The protection rate of different doses and median protective dose(PD₅₀) were 100%, 55%, 0%, 0%, 0.000 8 doses in the plume in vaccination group without TF, respectively, and 100%, 75%, 0%, 0%, 0.000 5 doses in the plume in vaccination group with TF. In contrast, the mortality of control group and blank group were 100% and 0%. After immunization with 10^-3 dose of vaccine, the levels of IL-4 and IFN-γ in vaccination group with TF were higher than in other groups and the difference was extremely significant on 7 and 14 dpv(P<0.01). After challenge, the levels of IL-4, IFN-γ and IL-12 P40 in vaccination group with TF were higher than in other groups. The differences of IL-4 on 1 and 14 days post challenge (dpc), IFN-γ on 1 and 3 dpc, IL-12 P40 on 1 dpc were extremely significant(P<0.01). These findings indicated that TF could reinforce cellular immunity by IFN-γ, IL-12 and humoral immunity by IL-4, improve the immune protection rate of NDV attenuated vaccine LaSota strain and reduce the PD₅₀. Vaccination group with TF was more effective than vaccination group without TF against virulent NDV strain F₄₈E₉.

Key words: pig spleen transfer factor, attenuated chicken NDV strain LaSota, virulent chicken NDV strain F₄₈E₉, immune protection rate, cytokine

CLC Number:

S858.315.3

XU Lei, ZHONG Jialian, YU Xunxin, LIU Yifa, HUANG Yu, LIU Xiaolong, LAI Longyong, YAN Liping, XU Xiumei, SONG Suquan, ZHANG Yuankui. Improvement of the Immune Protection Rate on Newcastle Disease Virus Attenuated Vaccine LaSota Strain by Pig Spleen Transfer Factor[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3578-3587.

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Improvement of the Immune Protection Rate on Newcastle Disease Virus Attenuated Vaccine LaSota Strain by Pig Spleen Transfer Factor

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