鸡TGFβ1对MDCC-MSB1细胞增殖、凋亡、迁移与侵袭的影响

doi:10.11843/j.issn.0366-6964.2020.10.025

Abstract

Abstract: This study aimed to detect the effect of Gallus TGFβ1 on the biological behavior of MDCC-MSB1 cells. MDCC-MSB1 cells were transiently transfected with Gallus TGFβ1 overexpression vector, interference expression vector, and the corresponding negative control. Then, the expression of Gallus TGFβ1, the cell proliferation, the cell cycle and apoptosis, the migration and invasion of each transfection groups were examined. Results showed that compared with the corresponding control, the MDCC-MSB1 cells transfected with overexpression vector of Gallus TGFβ1 could up-regulate the expression level of TGFβ1, the proliferation of MDCC-MSB1 cells was significantly inhibited, G1 phase cells were increased, S and G2 cells were decreased, the apoptosis rate of the cells was increased, the migration and invasion ability were decreased.However，the MDCC-MSB1 cells transfected with the interference expression vector of TGFβ1 significantly down-regulated the expression level of TGFβ1, cell proliferation was improved，G1 phase cells were decreased, S and G2 cells were increased, the cell apoptosis was decreased, the migration and invasion ability was increased. The results showed that Gallus TGFβ1 could inhibit the proliferation, migration and invasion of MDCC-MSB1 cells, and promote their apoptosis.

Key words: TGFβ1, MDCC-MSB1 cells, cell proliferation, apoptosis, migration, invasion

CLC Number:

YU Zuhua, DING Ke, JIA Yanyan, YU Chuan, HE Lei, LIAO Chengshui, LI Jing, ZHANG Chunjie, LI Yinju, WU Tingcai, CHENG Xiangchao, MENG Kaikai, ZHANG Jin, YU Zuling, ZHOU Ziyu, LUO Jun. Effect of Gallus TGFβ1 on the Proliferation, Apoptosis, Migration and Invasion of MDCC-MSB1 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2567-2575.

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Effect of Gallus TGFβ1 on the Proliferation, Apoptosis, Migration and Invasion of MDCC-MSB1 Cells

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