ChMDA5通路参与传染性法氏囊病病毒致雏鸡法氏囊损伤的研究

doi:10.11843/j.issn.0366-6964.2020.10.024

Abstract

Abstract: Infectious bursal disease virus (IBDV), a double-stranded RNA virus, can cause the infection of chickens with bursal lesions, leading to immunosuppression; melanoma differentiation-associated gene 5 (MDA5) is a pattern recognition receptor that specifically recognises double-stranded RNA viruses. In order to investigate the mechanism of the chicken MDA5 (chMDA5) signalling pathway in pathological injury in IBDV-infected chickens, fifty 14-day-old specific pathogen-free (SPF) chickens were allocated into 2 groups (infected and control) and twenty-five chickens were in each group. Each chicken of infected group was inoculated with 0.6 mL IBDV JIC7 virus solution intraocularly and intranasally. The chickens in control group were administered sterile phosphate buffered saline (PBS) in the same manner simultaneously. The chickens’ bursa of Fabricius was isolated at the 1st, 4th, 7th, 21st and 35th day after infection. Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the IBDV load, and mRNA expression of chMDA5, chMDA signal pathway adaptor protein (chIPS-1), transcription factor (chIRF3, chNF-κB) and downstream product cytokines (chIFN-β, chTNF-α, chIL-1β and chIL-6). Indirect immunofluorescence assay was performed for the expression of chMDA5 protein in chicken bursa of Fabricius. Conventional pathological methods were used to examine the pathological changes in the bursa of Fabricius. The results showed that the expression of chMDA5 as well as chIPS-1, chIRF3, chNF-κB, chIFN-β, chTNF-α, chIL-1β and chIL-6 were significantly higher than those in the control group, and morphological damage occurred in the bursal tissue in the infected group. The aforementioned trend was consistent with the change in IBDV load. The results indicated that chMDA5 and its signal transduction pathway could be activated by IBDV, which involves the bursa of Fabricius injury and anti-injury process in chickens infected with IBDV.

Key words: IBDV, bursa of Fabricius, chMDA5 signal transduction, pathology morphological damage, chicken

CLC Number:

S852.65⁺9.4

LUAN Ya'nan, XU Danlei, GE Ming, TANG Zequn, ZHAO Xia, ZHANG Ruili. Study on the Involvement of Chicken Melanoma Differentiation-Associated Gene 5 Pathway in Bursa Injury of Chicken Induced by Infectious Bursal Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2557-2566.

References 28

[1]	MULLER H,ISLAM R,RAUE R.Research on infectious bursal disease-the past,the present and the future[J].Vet Microbiol,2003,97(1-2):153-165.
[2]	王成成,葛铭,刘超男,等.IBDV感染雏鸡外周血液淋巴细胞chMDA5信号通路因子表达的动态变化[J].畜牧兽医学报,2015,46(5):830-835.WANG C C,GE M,LIU C N,et al.Dynamic changing in the expression of chMDA5 signal pathway factors in peripheral blood lymphocytes of chicken infected with IBDV[J].Acta Veterinaria et Zootechnica Sinica,2015,46(5):830-835.(in Chinese)
[3]	LEE C C,WU C C,LIN T L.Chicken melanoma differentiation-associated gene 5(MDA5) recognizes infectious bursal disease virus infection and triggers MDA5-related innate immunity[J].Arch Virol,2014,159(7):1671-1686.
[4]	YE C J,YU Z L,XIONG Y W,et al.STAU1 binds to IBDV genomic double-stranded RNA and promotes viral replication via attenuation of MDA5-dependent β interferon induction[J].FASEB J,2019,33(1):286-300.
[5]	BELGNAOUI S M,PAZ S,HISCOTT J.Orchestrating the interferon antiviral response through the mitochondrial antiviral signaling (MAVS) adapter[J].Curr Opin Immunol,2011,23(5):564-572.
[6]	KATO H,TAKEUCHI O,SATO S,et al.Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses[J].Nature,2006,441(7089):101-105.
[7]	栾亚男,葛铭,李广兴,等.雏鸡免疫器官中chMDA5的表达与定位[J].畜牧兽医学报,2016,47(7):1480-1487.LUAN Y N,GE M,LI G X,et al.The expression and location of chMDA5 in chick immune organs[J].Acta Veterinaria et Zootechnica Sinica,2016,47(7):1480-1487.(in Chinese)
[8]	LOUBER J,BRUNEL J,UCHIKAWA E,et al.Kinetic discrimination of self/non-self RNA by the ATPase activity of RIG-I and MDA5[J].BMC Biol,2015,13:54.
[9]	DEL TORO DUANY Y,WU B,HUR S.MDA5-filament,dynamics and disease[J].Curr Opin Virol,2015,12:20-25.
[10]	BARBER M R W,ALDRIDGE J R Jr,WEBSTER R G,et al.Association of RIG-I with innate immunity of ducks to influenza[J].Proc Natl Acad Sci U S A,2010,107(13):5913-5918.
[11]	KARPALA A J,STEWART C,MCKAY J,et al.Characterization of chicken Mda5 activity:regulation of IFN-β in the absence of RIG-I functionality[J].J Immunol,2011,186(9):5397-5405.
[12]	LINIGER M,SUMMERFIELD A,ZIMMER G,et al.Chicken cells sense influenza A virus infection through MDA5 and CARDIF signaling involving LGP2[J].J Virol,2012,86(2):705-717.
[13]	HAYASHI T,WATANABE C,SUZUKI Y,et al.Chicken MDA5 senses short double-stranded RNA with implications for antiviral response against avian influenza viruses in chicken[J].J Innate Immun,2014,6(1):58-71.
[14]	LEE C C,WU C C,LIN T L.Role of chicken melanoma differentiation-associated gene 5 in induction and activation of innate and adaptive immune responses to infectious bursal disease virus in cultured macrophages[J].Arch Virol,2015,160(12):3021-3035.
[15]	HANDA P,TUPPER J C,JORDAN K C,et al.FLIP (Flice-like inhibitory protein) suppresses cytoplasmic double-stranded-RNA-induced apoptosis and NF-κB and IRF3-mediated signaling[J].Cell Commun Signal,2011,9(1):16.
[16]	LEE N R,KIM H I,CHOI M S,et al.Regulation of MDA5-MAVS antiviral signaling axis by TRIM25 through TRAF6-mediated NF-κB activation[J].Mol Cells,2015,38(9):759-764.
[17]	LIM R,BARKER G,LAPPAS M.TLR2,TLR3 and TLR5 regulation of pro-inflammatory and pro-labour mediators in human primary myometrial cells[J].J Reprod Immunol,2017,122:28-36.
[18]	ZOU H T,SU R X,RUAN J,et al.Double-stranded RNA induces chicken T-cell lymphoma apoptosis by TRIF and NF-κB[J].Sci Rep,2017,7(1):7547.
[19]	ZHAN Q,SONG R,LI F,et al.Double-stranded RNA upregulates the expression of inflammatory mediators in human aortic valve cells through the TLR3-TRIF-noncanonical NF-κB pathway[J].Am J Physiol Cell Physiol,2017,312(4):C407-C417.
[20]	ZHAO C,DENISON C,HUIBREGTSE J M,et al.Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways[J].Proc Natl Acad Sci U S A,2005,102(29):10200-10205.
[21]	LI Z H,WANG Y Q,LI X,et al.Critical roles of glucocorticoid-induced leucine zipper in infectious bursal disease virus (IBDV)-induced suppression of type I Interferon expression and enhancement of IBDV growth in host cells via interaction with VP4[J].J Virol,2013,87(2):1221-1231.
[22]	KHATRI M,PALMQUIST J M,CHA R M,et al.Infection and activation of bursal macrophages by virulent infectious bursal disease virus[J].Virus Res,2005,113(1):44-50.
[23]	王灵娟.IBDV感染对SPF雏鸡免疫器官TGF-β1 mRNA表达和免疫功能影响[D].哈尔滨:东北农业大学,2013.WANG L J.The influence of infectious bursal disease virus to the TGF-β1 mRNA expression and Immune function in immune organs of SPF chicks[D].Harbin:Northeast Agricultural University,2013.(in Chinese)
[24]	HUTCHENS M,LUKER K E,SOTTILE P,et al.TLR3 increases disease morbidity and mortality from vaccinia infection[J].J Immunol,2008,180(1):483-491.
[25]	ZHANG S Y,JOUANGUY E,UGOLINI S,et al.TLR3 deficiency in patients with herpes simplex encephalitis[J].Science,2007,317(5844):1522-1527.
[26]	刘爵,韦莉,姚炜光,等.鸡传染性法氏囊病超强毒感染后SPF鸡免疫器官病理学观察[J].畜牧兽医学报,2002,33(4):371-376.LIU J,WEI L,YAO W G,et al.Pathological observation on immune organs of Special-Pathogen-Free(SPF) chicks inoculated with very virulent infectious Bursal disease virus[J].Acta Veterinaria et Zootechnica Sinica,2002,33(4):371-376.(in Chinese)
[27]	WEI L,HOU L,ZHU S S,et al.Infectious bursal disease virus activates the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway by interaction of VP5 protein with the p85α subunit of PI3K[J].Virology,2011,417(1):211-220.
[28]	LOMBARDO E,MARAVER A,ESPINOSA I,et al.VP5,the nonstructural polypeptide of infectious bursal disease virus,accumulates within the host plasma membrane and induces cell lysis[J].Virology,2000,277(2):345-357.

Study on the Involvement of Chicken Melanoma Differentiation-Associated Gene 5 Pathway in Bursa Injury of Chicken Induced by Infectious Bursal Disease Virus

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 28

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	NIU Jiajia, XU Dan, LIU Yang, ZHAO Xiaoling. Research Progress on Genetic Regulation Mechanism of Barring Feather Trait in Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1883-1892.
[2]	LEI Yanru, HU Xiaoyu, XU Chunhong, ZHANG Chenxi, DU Wenping, WANG Yangguang, LI Donghua, SUN Guirong, LI Wenting, KANG Xiangtao. Comparative Analysis of Growth, Carcass and Meat Quality Traits of Five Hybrid Combinations of Houdan Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1521-1535.
[3]	CAO Yuzhu, XING Yuxin, MA Chenglin, GUAN Hongbo, JIA Qihui, KANG Xiangtao, TIAN Yadong, LI Zhuanjian, LIU Xiaojun, LI Hong. Biological Characterization of Chicken FGF6 Gene and Association of Its Polymorphisms with Economic Traits [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1536-1550.
[4]	LUO Xiaoqin, GUO Zhiting, WANG He, ZHANG Jingyan, ZHANG Kang, WANG Lei, MA Yonghua, LI Jianxi. The Therapeutic Effect of Changshan Oral Liquid on Chicken Coccidiosis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1747-1755.
[5]	SU Chuanchen, JIAO Jingya, WANG Yongshuai, LUO Pengna, CHANG Xinghai, HUANG Yanqun. Effect of Introducing 25% Rhode Island Red Chicken Blood on Related Indexes of Henan Fighting Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1007-1018.
[6]	ZHENG Lin, WEI Bingdong, HUA Feng, CHEN Long, DING Yuan. Therapeutic Effect of Lytic Phage on Salmonella enteritidis Infection in Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1314-1327.
[7]	REN Yuwei, CHEN Xing, LIN Yanning, HUANG Xiaoxian, HONG Lingling, WANG Feng, SUN Ruiping, ZHANG Yan, LIU Hailong, ZHENG Xinli, CHAO Zhe. Investigating the Influencing Factors of Egg Laying Performance in Wenchang Chickens Based on Whole Genome Resequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 502-514.
[8]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[9]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[10]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, TAN Xiaodong, LIU Jie, ZHAO Guiping, WEN Jie, LIU Ranran. A Method for Quickly Mining the Characteristic SNP Markers Set of Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3252-3261.
[11]	CHEN Chun, KANG Zhaofeng, WEI Yue, LI Guanhong, WU Yanping, XIE Jinfang. Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2810-2823.
[12]	LIU Hang, WANG Huanhuan, GE Ying, ZHANG Lei, ZHANG Weiwu, WEI Yinghui, LI Qinghai, FAN Jinghui, ZHANG Xuedong. Screening of Candidate Genes of Skin Color of Black-Bone Chicken Based on Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2320-2329.
[13]	WANG Hui, XU Mengyuan, LIU Lingkang, ZHENG Xibang, LI Gonghe, WU Wende. PhiC31 Integrase Triggers Site-specific Cassette Exchange in Chicken Fibroblast Cells through Electroporation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2330-2342.
[14]	LIU Qing, WU Shaopeng, SHI Bin, SHAO Hongxia, QIAN Kun, YE Jianqiang, QIN Aijian. Expression of Chicken CTLA-4 Protein in Insect Cells and the Preparation of Its Monoclonal Antibody [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2596-2604.
[15]	WANG Zixuan, WANG Qiao, ZHANG Jin, Astrid Lissette Barreto Sánchez, ZHENG Maiqing, LI Qinghe, CUI Huanxian, AN Bingxing, ZHAO Guiping, WEN Jie, LI Hegang. Transcriptome Based Screening of Functional Genes Related to Heat Stress Resistance in Beijing You Chickens and Guangming Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1905-1914.