马立克病病毒编码的miR-M4-5p调控宿主靶基因的筛选及鉴定

doi:10.11843/j.issn.0366-6964.2020.08.020

Abstract

Abstract: Marek's disease, a rapid-onset neoplastic disease in poultry caused by the serotype 1 Marek's disease virus (MDV-1), has been historically regarded as an ideal model for the research on virally-induced cancers. Previous studies have demonstrated that the deletion of the miR-M4-5p, a viral analog of cellular oncogene miR-155 encoded by MDV-1, from the viral genome significantly reduces virus pathogenicity and oncogenicity, suggesting an important regulatory role responsible for MD tumorigenesis. To further reveal the regulatory mechanism mediated by miR-M4-5p in MD biology, we used CEF total cellular RNA as the template to produce a cDNA library utilizing the hybrid-PCR, for screening the host mRNA targets for miR-M4-5p. A total of 128 candidate host genes were obtained, of which there are 73 candidate binding sites were located in the 3'-UTRs and 23 of them contain the perfect matches of miRNA:mRNA. Further experiments of the dual luciferase reporter assay (DLRA) and the quantitative real-time PCR (qRT-PCR) analysis characterized three host genes, DPT, TMEM230 and DCLK1, as the in vivo targets for miR-M4-5p. Our work provides an important basis for further elucidating the regulatory mechanism of miR-M4-5p in MD oncogenesis.

Key words: Marek's disease virus, miRNA, host candidate target gene, oncogenesis, hybrid-PCR

CLC Number:

S852.659.1

SU Rui, TENG Man, LI Huizhen, ZHENG Luping, LIU Haoli, LIU Jinling, ZHU Zhijian, LUO Jun, ZHANG Gaiping. Screening and Characterization of the Host mRNA Targets for the miR-M4-5p Encoded by Marek's Disease Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1956-1969.

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Screening and Characterization of the Host mRNA Targets for the miR-M4-5p Encoded by Marek's Disease Virus

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