日粮中添加双氢青蒿素对宫内发育迟缓断奶仔猪胰岛素水平和肝发育的影响

doi:10.11843/j.issn.0366-6964.2020.07.015

Abstract

Abstract: In this study we aimed to investigate the effects of intrauterine growth retardation (IUGR) on insulin levels and hepatic development in weaned piglets, and the repairing effect of dietary supplementation with 80 mg·kg^-1 dihydroartemisinin (DHA). Ten piglets with normal birth weight (NBW) and 20 IUGR piglets were selected and fed either a basal diet (NBW and IUGR groups, 10 piglets in each gorup) or the basal diet supplemented with 80 mg·kg^-1 DHA (IUGR-DHA group, 10 piglets) for 29 days from 21 to 49 d of age. Compared with NBW group, IUGR group significantly decreased the serum content of fasting glucose (FBG) and insulin-like growth factor-1 (IGF-1) and the hepatic weight (P<0.05), and significantly increased the content of fasting insulin (FINS) and homeostasis model assessment of insulin resistance (HOMA-IR) values and hepatic index (P<0.05). At the same time, the morphology and structure of the hepatic tissue in IUGR weaned piglets were damaged, there was obvious vacuolation, and organelle structure was damaged, mitochondria and endoplasmic reticulum were significantly swollen, and the nucleus was seriously deformed. Compared with IUGR group, IUGR-DHA group significantly increased the hepatic weight and IGF-1 content in the serum (P<0.05). The serum content of FINS and HOMA-IR values were significantly decreased (P<0.05). In addition, the morphology and structure of liver tissue were significantly improved. Compared with NBW group, IUGR group significantly downregulated the relative mRNA expression of IGF-1, insulin receptor substrate 1 (IRS1), phosphatidylinositol 3-kinase (PI3K) and protein kinase B (AKT2) (P<0.05) in liver. Compared with IUGR group, IUGR-DHA group significantly upregulated the relative mRNA expression of IRS1 and AKT2 in the liver of IUGR weaned piglets (P<0.05). The results showed that DHA had a certain repairing effect on insulin resistance and hepatic development damage of weaned piglets caused by IUGR. This study provides a theoretical basis for the early treatment of IUGR and the promotion and application of DHA in livestock production.

Key words: dihydroartemisinin, intrauterine growth retardation, liver, insulin resistance

CLC Number:

S829.9+1

ZHAO Yongwei, NIU Yu, HE Jintian, ZHANG Lili, WANG Chao, WANG Tian. Effects of Dietary Dihydroartemisinin Supplementation on Insulin Level and Liver Development in Intrauterine Growth Retardation Weaned Piglets[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1628-1636.

References 35

[1]	RESNIK R.Intrauterine growth restriction[J].Obstet Gynecol,2002,99(3):490-496.
[2]	POLLACK R N,DIVON M Y.Intrauterine growth retardation:definition,classification,and etiology[J].Clin Obstet Gynecol,1992,35(1):99-107.
[3]	SANKARAN S,KYLE P M.Aetiology and pathogenesis of IUGR[J].Best Pract Res Clin Obstet Gynaecol,2009,23(6):765-777.
[4]	WU G,BAZER F W,WALLACE J M,et al.Board-invited review:intrauterine growth retardation:implications for the animal sciences[J].J Anim Sci,2006,84(9):2316-2337.
[5]	WANG Y X,ZHANG L L,ZHOU G L,et al.Dietary L-arginine supplementation improves the intestinal development through increasing mucosal Akt andmammalian target of rapamycin signals in intra-uterine growth retarded piglets[J].Br J Nutr,2012,108(8):1371-1381.
[6]	YING Z X,ZHANG H,SU W P,et al.Dietary methionine restriction alleviates hyperglycemia in pigs with intrauterine growth restriction by enhancing hepatic protein kinase b signaling and glycogen synthesis[J].J Nutr,2017,147(10):1892-1899.
[7]	MORTEZA A,ABDOLLAHI A,BANDARIAN M.Serum nitric oxide syntheses and lipid profile of the mothers with IUGR pregnancies uncomplicated with preeclampsia.Does insulin resistance matter?[J].Gynecol Endocrinol,2012,28(2):139-142.
[8]	HE J T,DONG L,XU W,et al.Dietary tributyrin supplementation attenuates insulin resistance and abnormal lipid metabolism in suckling piglets with intrauterine growth retardation[J].PLoS One,2015,10(8):e0136848.
[9]	ZHANG J F,XU W,HAN H L,et al.Dietary leucine supplementation restores serum glucose levels,and modifying hepatic gene expression related to the insulin signal pathway in IUGR piglets[J].Animals,2019,9(12):1138.
[10]	常明,张相林,刘晓,等.双氢青蒿素人体药动学及生物等效性研究[J].中国药房,2006,17(14):1086-1088.CHANG M,ZNANG X L,LIU X,et al.Study on pharmacokinetics and bioavailability of dihydroartemisinin in human body[J].China Pharmacy,2006,17(14):1086-1088.(in Chinese)
[11]	喻婉莹,阚伟娟,于鹏霞,等.青蒿素和二氢青蒿素的抗炎作用及机制[J].中国中药杂志,2012,37(17):2618-2621.YU W Y,KAN W J,YU P X,et al.Anti-inflammatory effect and mechanism of artemisinin and dihydroar-temisinin[J].China Journal of Chinese Materia Medica,2012,37(17):2618-2621.(in Chinese)
[12]	LU P,ZHANG F C,QIAN S W,et al.Artemisinin derivatives prevent obesity by inducing browning of WAT and enhancing BAT function[J].Cell Res,2016,26(10):1169-1172.
[13]	ZHAO X Y,WANG L Q,ZHANG H,et al.Protective effect of artemisinin on chronic alcohol induced-liver damage in mice[J].Environ Toxicol Pharmacol,2017,52:221-226.
[14]	李敏,金晓琨,李卫东.双氢青蒿素保护刀豆蛋白A诱导的小鼠肝损伤及机制探讨[J].中国药理学通报,2009,25(5):630-633.LI M,JIN X K,LI W D.The protective effect of DQHS on ConA-induced liveRinjury in mice and its mechanism[J].Chinese Pharmacological Bulletin,2009,25(5):630-633.(in Chinese)
[15]	CHE L Q,XUAN Y,HU L,et al.Effect of postnatal nutrition restriction on the oxidative status of neonates with intrauterine growth restriction in a pig model[J].Neonatology,2015,107(2):93-99.
[16]	YIN J H,LI M,XU L,et al.Insulin resistance determined by Homeostasis Model Assessment (HOMA) and associations with metabolic syndrome amongChinese children and teenagers[J].Diabetol Metab Syndr,2013,5(1):71.
[17]	曲晓洁,白雪山,吴秀英,等.葡萄糖转运蛋白2和4在自发高尿酸血症小鼠发生胰岛素抵抗中的作用[J].中华内分泌代谢杂志,2018,34(10):862-866.QU X J,BAI X S,WU X Y,et al.Role of glucose transporter 2 and 4 ininsulin resistance in spontaneous hyperuricemia mice[J].Chinese Journal of Endo-crinology and Metabolism,2018,34(10):862-866.(in Chinese)
[18]	邓兴照,彭华,刘福柱.类胰岛素生长因子-Ⅰ及其营养调控[J].中国畜牧杂志,2005,41(3):39-41.DENG X Z,PENG H,LIU F Z.IGF-Ⅰ and nutrition modulation[J].Chinese Journal of Animal Science,2005,41(3):39-41.(in Chinese)
[19]	YAKAR S,LIU J L,FERNANDEZ A M,et al.Liver-specific igf-1 gene deletion leads to muscle insulin insensitivity[J].Diabetes,2001,50(5):1110-1118.
[20]	BAKER J,LIU J P,ROBERTSON E,et al.Role of insulin-like growth factors in embryonic and postnatal growth[J].Cell,1993,75(1):73-82.
[21]	NIU Y,HE J T,AHMAD H,et al.Curcumin atte-nuates insulin resistance and hepatic lipidaccumulation in a rat model of intra-uterine growth restriction through insulin signalling pathway and sterol regulatory element binding proteins[J].Br J Nutr,2019,122(6):616-624.
[22]	HARA K,YONEZAWA K,SAKAUE H,et al.1-Phosphatidylinositol 3-kinase activity is required for insulin-stimulated glucose transport but not for RAS activation in CHO cells[J].Proc Natl Acad Sci USA,1994,91(16):7415-7419.
[23]	CARVALHO E,JANSSON P A,AXELSEN M,et al.Low cellular IRS 1 gene and protein expression predict insulin resistance and NIDDM[J].FASEB J,1999,13(15):2173-2178.
[24]	DOMINICI F P,HAUCK S,ARGENTINO D P,et al.Increased insulin sensitivity and upregulation of insulin receptor,insulin receptor substrate (IRS)-1 and IRS-2 in liver of Ames dwarf mice[J].J Endocrinol,2002,173(1):81-94.
[25]	LIAO K,LI J,WANG Z L.Dihydroartemisinin inhi-bits cell proliferation via AKT/GSK3β/cyclinD1 pathway and induces apoptosis in A549 lung cancer cells[J].Int J Clin Exp Pathol,2014,7(12):8684-8691.
[26]	何进田,董丽,白凯文,等.三丁酸甘油酯对宫内发育迟缓哺乳仔猪肝脏抗氧化和线粒体功能的影响[J].食品科学,2016,37(3):191-196.HE J T,DONG L,BAI K W,et al.Effect of tributyrin on liver antioxidant capacity and mitochondrial function of piglets with intrauterine growth retardation during sucking period[J].Food Science,2016,37(3):191-196.(in Chinese)
[27]	赵永伟,牛玉,何进田,等.双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响[J].畜牧兽医学报,2019,50(10):2139-2146.ZHAO Y W,NIU Y,HE J T,et al.Effects of dihydroartemisinin on oxidative stress in liver of weaned piglets induced by lipopolysaccharide[J].Acta Veterinaria et Zootechnica Sinica,2019,50(10):2139-2146.(in Chinese)
[28]	程林,谌俊,韩景河,等.母猪妊娠和哺乳饲粮中硒源和维生素E水平对后代仔猪器官指数和血清激素水平的影响[J].动物营养学报,2018,30(3):918-928.CHENG L,CHAN J,HAN J H,et al.Effects of dietary selenium sources and vitamin e levels during sows' gestation and lactation on organ indices and serum hormone levels of their progeny[J].Chinese Journal of Animal Nutrition,2018,30(3):918-928.(in Chinese)
[29]	周盟,张乃锋,董晓丽,等.益生菌对断奶仔猪生长性能、免疫器官指数及胃肠道pH的影响[J].动物营养学报,2014,26(2):445-452.ZHOU M,ZHANG N F,DONG X L,et al.Effects of probiotics on growth performance,immune organ indexes and gastrointestinal pH of Weaner Piglets[J].Chinese Journal of Animal Nutrition,2014,26(2):445-452.(in Chinese)
[30]	BAUER R,WALTER B,HOPPE A,et al.Body weight distribution and organ size in newborn swine (sus scrofa domestica)-A study describing an animal model for asymmetrical intrauterine growth retardation[J].Exp Toxicol Pathol,1998,50(1):59-65.
[31]	何进田,董丽,张莉莉,等.三丁酸甘油酯对宫内发育迟缓哺乳仔猪肝脏发育和免疫功能的影响[J].南京农业大学学报,2015,38(5):838-843.HE J T,DONG L,ZHANG L L,et al.Effects of tri-butyrin on the development and immune function of the liver in the intrauterine growth restricted suckling piglets[J].Journal of Nanjing Agricultural University,2015,38(5):838-843.(in Chinese)
[32]	封飞飞,方伟,王淑楠,等.茶树油对育肥猪生长性能、器官指数、胴体性状和肉品质的影响[J].动物营养学报,2017,29(10):3620-3626.FENG F F,FANG W,WANG S N,et al.Effects of tea tree oil on growth performance,organ indexes,carcass characteristics and meat quality of finishing pigs[J].Chinese Journal of Animal Nutrition,2017,29(10):3620-3626.(in Chinese)
[33]	冯程程,白凯文,王安谙,等.日粮添加二甲基甘氨酸钠对宫内发育迟缓断奶仔猪肝脏抗氧化能力及免疫指标的影响[J].南京农业大学学报,2019,42(2):336-344.FENG C C,BAI K W,WANG A A,et al.Effect of N,N-dimethylglycine sodium salt supplementation on hepatic antioxidant capacity and immune indices in intrauterine growth retardation weanling piglets[J].Journal of Nanjing Agricultural University,2019,42(2):336-344.(in Chinese)
[34]	HE J T,NIU Y,WANG F,et al.Dietary curcumin supplementation attenuates inflammation,hepatic injury and oxidative damage in a rat model of intra-uterinegrowth retardation[J].Br J Nutr,2018,120(5):537-548.
[35]	ZHANG H,SU W P,YING Z X,et al.N-acetylcysteine attenuates intrauterine growth retardation-induced hepatic damage in suckling piglets by improving glutathione synthesis and cellular homeostasis[J].Eur J Nutr,2018,57(1):327-338.

Effects of Dietary Dihydroartemisinin Supplementation on Insulin Level and Liver Development in Intrauterine Growth Retardation Weaned Piglets

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 35

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHANG De'an, YANG Ruozhu, LIU Jie, LIU Dewu, DENG Ming, LIU Guangbin, SUN Baoli, GUO Yongqing, LI Yaokun. Expression Analysis of Transcriptome in the Liver of Chuanzhong Black Goats Fed with Silage Neolamarckia Cadamba Substitute for Silage Corn [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 232-244.
[2]	MA Yajun, JIAO Zhihui, LIU Xiaoning, LU Xiangyu, LIU Tao, WANG Yue, PIAO Chenxi, WANG Hongbin. Effects of Adipose-derived Mesenchymal Stem Cells on Pyroptosis of Miniature Pigs with Hepatic Ischemia-Reperfusion Combined with Hepatectomy [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 355-364.
[3]	ZHANG Yafeng, ZHU Bin, MA Chang, ZHANG Yuanshu. The Research on the Effects of ACE2 Activated by Diminazene Aceturate on Mitochondria in the Liver of Rats with Non-alcoholic Fatty Liver Disease [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3895-3904.
[4]	YUAN Heling, FANG Ci, HUANG Jinhu, WANG Xiaoming, XIAO Wenjun, LIU Ruiting, SHI Rongmei, WANG Liping. Effects of Allicin on mRNA Expression of CYP1A2 and Its Enzyme Kinetics in Broilers Liver Tissue [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3905-3915.
[5]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[6]	ZHAO Yangfei, YU Yanghuan, WANG Jinming, ZHANG Jianhai, SUN Zilong, NIU Ruiyan, WANG Jundong. Effects of IL-17A Knockout on Fluoride-Induced Hepatic Inflammation and Hepatocyte Apoptosis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3108-3117.
[7]	GENG Meimei, DOU Mengying, FU Dezhi, HE Qinghua, KONG Xiangfeng. Effects of Intrauterine Growth Retardation on Developmental Model of Insulin-Like Growth Factor in Suckling Piglets of Huanjiang Mini-Pig [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2414-2420.
[8]	ZHAO Donghao, YUAN Meng, MA Kaiteng, DUAN Zhuo, ZHU Yixin, TANG Fang, HAN Keguang, HUO Nairui. Chelating Role of Sheep Bone Collagen Peptide to Cadmium and Its Protection Role against Liver Injuries Induced by Cadmium in Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2641-2652.
[9]	SUN Xiaojing, ZHANG Lei, TIAN Tian, MA Xi, YAO Jia, WANG Yang. Unravelling Toxoplasma Treatment: Conventional Drugs toward Nanomedicine [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1834-1844.
[10]	OU Zhengmiao, ZHOU Jiawen, LIU Lili, WU Yun, CHEN Fenfen. Screening and Expression Analysis of Genes Related to Lipid Metabolism in Liver Tissue of Wuliangshan Sooty Chicken Based on RNA-Seq [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 976-988.
[11]	SUN Meijie, CAO Liwen, ZHENG Wenjin, SHEN Junshi, ZHU Weiyun. Effect of Dietary Urea Supplementation on Liver Ammonia Metabolism in Fattening Hu Lambs Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1148-1159.
[12]	CHEN Jingyi, YU Miao, ZHANG Jinyang, FAN Kun, YANG Guijun, GE Ming, ZHANG Ruili. Study on the Involvement of Ferroptosis in Liver Injury of Cadmium-exposed Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 787-802.
[13]	ZHAO Huiying, YU Shiqiang, ZHAO Yuchao, JIANG Linshu. Mechanism of Liver-Adipose Tissue Crosstalk in the Development of Fatty Liver in Periparturient Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4105-4116.
[14]	ZHOU Hui, GUAN Peng, WANG Chenyu, HOU Ruoxin, LI Tiejun, YIN Yulong, HE Liuqin. Effects of Serine on Growth Performance, Intestinal Morphology and Immune Related Indexes of Suckling Piglets with Intrauterine Growth Retardation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4220-4232.
[15]	WEI Zhangyi, HAN Fei, FAN Tingting, CHEN Zixian, LI Hanmei, YUAN Feifan, CONG Rihua, LI Xian. Regulation of Kushen-cangzhu Granules on Hepatic Cholesterol Metabolism in Broilers Infected with Escherichia coli [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 392-402.