山羊妊娠早期胎儿胎盘血管形成及相关基因的表达

doi:10.11843/j.issn.0366-6964.2020.05.013

Abstract

Abstract: The study aimed to investigate the development and distribution of blood vessels and the expression of angiogenesis-related factors in fetal placenta of Dazu Black goat during early pregnancy, and to analyze the correlation between the expression of angiogenesis-related factors and vascular development. Fifteen 8-month-old, healthy, young female Dazu Black goats were randomly selected, after natural mating with the same ram (recorded as 0 d on the last breeding day), fetuses and fetal placenta were collected by cesarean operation at 20, 25, and 30 d of pregnancy, and fetuses and fetal placenta were collected after slaughter at 45 and 60 d of pregnancy. Placental vascular development and distribution were investigated by immunohistochemical technology, and the expression level of angiogenesis-related genes was detected by qRT-PCR technique, and the correlation between the expression of angiogenesis-related genes and the placental vascular distribution was analyzed. The results showed that fetal body weight and body length increased gradually with the progress of pregnancy, and the positive rate of vascular endothelial cells was at a high level (>26%). With the progress of pregnancy, goat fetal placenta vascularity area/tissue area(CAD) gradually increased; capillary number/tissue area (CND) increased during 25-30 d and decreased during 30-60 d of pregnancy; Average capillary area (APC) showed an opposite trend to CND; There was no significant change in capillary circumference/tissue area(CSD). The expression of VEGFA in fetal placenta was gradually increased with the progress of pregnancy and correlated with vascular distribution. The expression level of FLT1 and KDR in fetal placenta first increased and then decreased, reaching the peak at 30 and 45 d of pregnancy, respectively. The expression of ANGPT1/2 and its receptor TEK, FGF2 and its receptor FGFR2 were higher at 30 d of pregnancy, which could be related to angiogenesis. In conclusion, before 30 d of pregnancy, fetal placenta mainly formed branches through capillaries to promote the increase of vascular area; at 30-60 d of pregnancy, the increase of vascular area was mainly promoted by the thickening of blood vessels per unit, and the expression of angiogenesis-related genes was higher at 30 d of pregnancy, which might be related to cotyledon formation. VEGFA in goat fetal placenta during early pregnancy could act together with other angiogenesis-related factors to regulate the angiogenesis.

Key words: Dazu Black goat, early pregnancy, fetal placenta, angiogenesis, gene expression

CLC Number:

S827.3

CHENG Wenqiang, WANG Pan, LUO Nanjian, XU Huizhong, WANG Wei, ZHANG Rong, CHENG Lei, ZHAO Wei, ZHAO Yongju. Detection of Fetal Placental Blood Vessels and Expression of Angiogenesis-Related Genes in Dazu Black Goats during Early Pregnancy[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5): 1019-1029.

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Detection of Fetal Placental Blood Vessels and Expression of Angiogenesis-Related Genes in Dazu Black Goats during Early Pregnancy

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