痛风雏鹅肾组织损伤的RNA-seq分析

doi:10.11843/j.issn.0366-6964.2020.03.024

Abstract

Abstract: We used RNA-seq to measure the transcription differences in renal tissues between gout and healthy goslings. Five goslings with typical visceral gout characteristics were selected as "gout group", while six healthy goslings were selected as the "control group". As a result, the transcription expressions of gout goslings in renal tissues were significantly different from that of healthy goslings. A total of 1 749 differential expression genes were found. Among these, 501 genes were up-regulated and 1 248 genes were down-regulated in the gout group. The GO and KEGG search revealed the differential expression genes mainly enriched in chemokine activity, T cell receptor binding, antigen binding and immune response, and participate in processes of cytokine-cytokine receptor interaction, intestinal immune network for IgA production, cell adhesion and so on.

Key words: gosling gout, renal injury, RNA-seq, immune response, inflammation damage

CLC Number:

S856.5

SHAO Chunrong, HUANG Yuanpi, HUANG Yunmao, YING Shijia, XI Yumeng, SHI Zhendan. RNA-seq Analysis on the Renal Tissue Injury in the Development of Goslings Gout[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 639-643.

References 14

[1]	奚雨萌, 闫俊书, 应诗家, 等. 高蛋白质高钙饲粮对雏鹅内脏型痛风发生、肾脏功能及肠道微生物区系的影响[J]. 动物营养学报, 2019, 31(2):612-621.XI Y M, YAN J S, YING S J, et al. Effects of high protein and calcium diets on visceral gout development, kidney function and intestinal microbial community of goslings[J]. Chinese Journal of Animal Nutrition, 2019, 31(2):612-621. (in Chinese)
[2]	XI Y M, YAN J S, LI M Y, et al. Gut microbiota dysbiosis increases the risk of visceral gout in goslings through translocation of gut-derived lipopolysaccharide[J]. Poultry Sci, 2019, 98(11):5361-5373.
[3]	JIN M L, WANG X Y, NING K, et al. Genetic characterization of a new astrovirus in goslings suffering from gout[J]. Arch Virol, 2018, 163(10):2865-2869.
[4]	朱书梁. 高钙和高钙高蛋白日粮对鸡临床病理学及相关基因表达的影响[D]. 南昌:江西农业大学, 2014.ZHU S L. Effects of diets with high calcium and high calcium-high protein on clinical pathology and expression of related genes[D]. Nanchang:Jiangxi Agricultural University, 2014. (in Chinese)
[5]	SHAO T J, SHAO L, LI H C, et al. Combined signature of the fecal microbiome and metabolome in patients with gout[J]. Front Microbiol, 2017, 8:268.
[6]	奚雨萌, 闫俊书, 应诗家, 等. 雏鹅痛风发病原因及其防控技术[J]. 中国家禽, 2018, 40(23):63-66.XI Y M, YAN J S, YING S J, et al. Causes and countermeasures of gout in goslings[J]. China Poultry, 2018, 40(23):63-66. (in Chinese)
[7]	GUO Z, ZHANG J C, WANG Z L, et al. Intestinal microbiota distinguish gout patients from healthy humans[J]. Sci Rep, 2016, 6(1):20602.
[8]	PENFOLD R S, PRENDECKI M, MCADOO S, et al. Primary IgA nephropathy:current challenges and future prospects[J]. Int J Nephrol Renovasc Dis, 2018, 11:137-148.
[9]	PRASAD N, PATEL M R. Infection-induced kidney diseases[J]. Front Med (Lausanne), 2018, 5:327.
[10]	CHAWLA R, VAN PUYENBROECK V, PFLUG N C, et al. Tuning side arm electronics in unsymmetrical cyclotriazadisulfonamide (CADA) endoplasmic reticulum (ER) translocation inhibitors to improve their human cluster of differentiation 4(CD4) receptor down-modulating potencies[J]. J Med Chem, 2016, 59(6):2633-2647.
[11]	SCHMIDT M E, VARGA S M. The CD8 T cell response to respiratory virus infections[J]. Front Immunol, 2018, 9:678.
[12]	FRICK K K, BUSHINSKY D A. Molecular mechanisms of primary hypercalciuria[J]. J Am Soc Nephrol, 2003, 14(4):1082-1095.
[13]	MACOSKA J A, WANG Z Y, VIRTA J, et al. Inhibition of the CXCL12/CXCR4 axis prevents periurethral collagen accumulation and lower urinary tract dysfunction in vivo[J]. Prostate, 2019, 79(7):757-767.
[14]	MITTAZ L, RICARDO S, MARTINEZ G, et al. Neonatal calyceal dilation and renal fibrosis resulting from loss of Adamts-1 in mouse kidney is due to a developmental dysgenesis[J]. Nephrol Dial Transplant, 2005, 20(2):419-423.

[1]	LIU Yangguang, ZHANG Huibin, WEN Haoyu, XIE Fan, ZHAO Shiming, DING Yueyun, ZHENG Xianrui, YIN Zongjun, ZHANG Xiaodong. SNP/Indel Screening Analysis of Porcine Ovarian Granulosa Cells Treated with Follicular Fluid Exosomes [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 576-586.
[2]	QIU Wenyue, SU Yiman, YE Jiali, ZHANG Xinting, PANG Xiaoyue, WANG Rongmei, XIE Zimao, ZHANG Hui, TANG Zhaoxin, SU Rongsheng. Study on Asiatic Acid Alleviates LPS-induced Acute Kidney Injury by Regulating Apoptosis and Autophagy of Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 809-821.
[3]	MIAO Shu, AN Jishan, WANG Zuo, XIAO Dingfu, LAN Xinyi, LIU Lei, SHEN Weijun, WAN Fachun. Leucine Promotes the Proliferation of Bovine Myoblasts through PI3K-AKT Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 142-152.
[4]	LIANG Kaixin, ZHONG Haiwen, SONG Changxu, YANG Huaqiang, HUANG Sixiu, XU Zheng. Study on the Effect of SYNGR2 on PCV2 Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3824-3835.
[5]	ZHONG Hua, SONG Shanshan, SHAO Huanting, ZHAO Yu, KANG Jinwen, WU Yao, SU Renwei. Transcriptome Sequencing Analysis on Canine Pyometra Uterine Tissue [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3383-3392.
[6]	WANG Jiandong, TANG Yulin, WANG Min, ZHANG Baosuo, YANG Fuqiang, GAO Haihui, YU Yang, GUO Yansheng. The Mechanism of Lycium barbarum Polysaccharide against Immunosuppression Induced by Cyclophosphamide in Chicks Based on RNA-Seq Technique [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3519-3532.
[7]	CHEN Xin, QIN Tong. mRNA Vaccine and Its Research Prospect in Zoonotic Diseases [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2732-2742.
[8]	LONG Qinqin, WEI Min, WANG Yuting, WEN Ming, PANG Feng. The Battle between Orf Virus and Host: Immune Response and Viral Immune Evasion Mechanisms [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1845-1853.
[9]	OU Zhengmiao, ZHOU Jiawen, LIU Lili, WU Yun, CHEN Fenfen. Screening and Expression Analysis of Genes Related to Lipid Metabolism in Liver Tissue of Wuliangshan Sooty Chicken Based on RNA-Seq [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 976-988.
[10]	ZHANG Gaomeng, DING Jiqiang, LIU Yuhong, ZHENG Maiqing, WEN Jie, ZHAO Guiping, LI Qinghe. Genome-wide Association Study Reveals the Genetic Basis of Hatching Traits in White-feathered Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 534-544.
[11]	ZHANG Wentao, LIU Chenyang, ZHU Binglin, LIU Li, TIAN Yuan, YAO Yuhang, CHENG Gong. Study on the Effects and Mechanisms of Snail1 on Proliferation and Differentiation of Bovine Adipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5008-5019.
[12]	CAI Jiawei, ZHANG Chen, JIN Rongshuai, BAO Zhiyuan, ZHANG Xiyu, WANG Fan, ZHAI Pin, ZHAO Bohao, CHEN Yang, TANG Xianwei, WU Xinsheng. Analysis of Testicular Tissue Morphology and Semen Transcriptome of Male Rabbits under Heat Stress [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4653-4663.
[13]	ZHANG Dongjie, WANG Liang, MA Hong, LI Zhongqiu, WANG Wentao, LIU Di. Analysis of Transcriptional Regulation in Min Pig Skeletal Muscle under Low Temperature Stress [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2524-2536.
[14]	WU Chunlin, ZHONG Lemiao, ZHAO Yan, LI Wenji, HUANG Xiaozi, WU Yijian. Transcriptomic Analysis on Responses of Chicken Trachea to Mycoplasma gallisepticum Strain MG-HY Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2652-2662.
[15]	ZHAO Xuyang, JIN Jiaxin, LU Wenlong, ZHANG Shuai, HUANG Li, ZHANG Gaiping, SUN Aijun, ZHUANG Guoqing. Advances in the Molecular Mechanism of Immune Escape of African Swine Fever Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2074-2082.

RNA-seq Analysis on the Renal Tissue Injury in the Development of Goslings Gout

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 14

Related Articles 15

Recommended Articles

Metrics

Comments