全基因组关联分析揭示白羽肉鸡孵化性状的遗传基础

doi:10.11843/j.issn.0366-6964.2023.02.011

Abstract

Abstract: The study aimed to reveal the genetic basis of hatching traits of white-feathered broilers. In this experiment, two groups of White-feather broilers A and B were used as materials to determine the fertilization rate and hatching rate of 40-week-old eggs in 5 generations (556) of group A and 3 generations (398) of group B. The 55K SNP chip was used to genotype 954 healthy individuals in the two groups. Genetic parameter estimation based on pedigree and single nucleotide polymorphisms (SNPs) showed that the heritability of fertilization rate of group A was 0.21 and 0.14, respectively. Genome-wide association study (GWAS) of fertilization rate and hatching rate was performed using mixed linear model. A total of 12 significant SNPs were obtained by genome-wide association study, distributed on chromosome 1, 9, 11, 12, 20. Among them, 9 SNPs were significantly associated with fertilization rate, 3 SNPs were significantly associated with hatching rate, RNA-seq was performed on testis tissues of 4 roosters in each group with high or low fertilization rate, 2 differentially expressed genes were identified by qPCR. Nine genes related to fertilization rate were identified by genome-wide association study, including COPG1, ADAMTS18, FABP2, CDH8, SLCO4A1, ATP13A3, NELL2, VEZT and IGHMBP2,3 genes related to hatching rate, including TMEM, SCO1 and MYH1A, RNA-seq and qPCR identified two candidate genes, HMGCLL1 and COA6, which were related to fertilization rate. In this study, GWAS and RNA-seq were used to identified candidate genes related to hatching traits, which provided important molecular markers for the genetic improvement of hatching traits of White-feathered broilers.

Key words: White-feather broiler, fertilization rate, hatching rate, GWAS, RNA-seq

CLC Number:

S831.2

ZHANG Gaomeng, DING Jiqiang, LIU Yuhong, ZHENG Maiqing, WEN Jie, ZHAO Guiping, LI Qinghe. Genome-wide Association Study Reveals the Genetic Basis of Hatching Traits in White-feathered Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 534-544.

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Genome-wide Association Study Reveals the Genetic Basis of Hatching Traits in White-feathered Broilers

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