SYNGR2影响猪圆环病毒2型体外增殖的研究

doi:10.11843/j.issn.0366-6964.2023.09.022

Abstract

Abstract: Synaptogyrin-2 (SYNGR2) protein affects PCV2 infection in host. This study aims to investigate the SYGNR2 function on PCV2 proliferation in cell cultures and dissect the mechanism for SYNGR2 affecting PCV2 infection. We performed gene knockout, overexpression and site-directed mutagenesis assay to study how SYNGR2 affects PCV replication in PK15 cells. To investigate the mechanism, RNA-seq was performed to study the molecular differences between SYNGR2 KO and WT cells. Differentially expressed genes between the two groups were analyzed and further validated by quantitative Real-time PCR. SYNGR2 knockout and p.Arg63Cys point mutation decreased PCV2 replication in PK15 cells, and the overexpression of different genotypes of SYNGR2 restored PCV2 replication capacity in SYNGR2 KO cells. RNA-seq enrichment analysis showed that the SYNGR2 mainly participates in the membrane components and vesicles and other membrane organelles. The differentially expressed gene MYRIP and its bound exosome regulation related gene RAB27A were selected for verification, and qPCR results showed that the expression of RAB27A mRNA was significantly increased in SYNGR2 knockout cells.In Conclusion, this report confirmed SYNGR2 function to support PCV2 infection in cells. SYNGR2 knockout and p.Arg63Cys point mutation both decreased PCV2 replication in cells. Transcriptome analysis reveals that SYNGR2 may affect PCV2 replication capacity by regulating the function of vesicular transport.

Key words: SYNGR2, PCV2, RNA-seq, RAB27A, vesicle

CLC Number:

S855.3

LIANG Kaixin, ZHONG Haiwen, SONG Changxu, YANG Huaqiang, HUANG Sixiu, XU Zheng. Study on the Effect of SYNGR2 on PCV2 Replication[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3824-3835.

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Study on the Effect of SYNGR2 on PCV2 Replication

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