QDPR基因克隆及其在乌蒙凤鸡不同生长阶段组织表达研究

doi:10.11843/j.issn.0366-6964.2020.004

Abstract

Abstract: The aim of this study was to clone quinoid dihydropteridine reductase (QDPR) gene and detect its expression in different tissues, different growth stages of Wumeng Crested chicken of different genders, so as to study the regulatory role of QDPR gene in the growth and development of Wumeng Crested chicken. In this experiment, the CDS region of QDPR gene of Wumeng Crested chicken was amplified and cloned by PCR, bioinformatics analysis of the sequences was carried out, and real-time fluorescent quantitative PCR analysis was performed to detect the expression of QDPR gene in heart, liver, spleen, lung, kidney, chest muscle and leg muscle of Wumeng Crested chicken in 4, 12, 20, and 28 weeks old. The results showed that the length of the open reading frame of QDPR gene was 417 bp, and 139 amino acids were encoded in total. The encoding protein was acidic and unstable. The QDPR gene sequence of Wumeng Crested chicken was highly homologous with the Gallus gallus, Coturnix japonica and Numida meleagris; QDPR gene was expressed in all tissues of Wumeng Crested chickens of different genders at different stages, the relative expression level of QDPR gene was the highest in the lung tissue of cock in 4,12 and 20 weeks old, which was extremely significantly higher than that in other tissues at the same age (P<0.01). The relative expressions of QDPR gene in liver, spleen and lung of 4-week-old hens were the highest in all stages of growth, which was extremely significantly higher than those in the stages of 12- and 20-week-old (P<0.01). The relative expressions of QDPR gene in the most of tissues of cock generally showed a trend of increasing first and then decreasing with time, while decreasing first and then increasing in hens. The results indicated that the expressions of QDPR gene in visceral tissues of Wumeng Crested chickens were higher than that in muscle tissues, and the expression in cock and hen were different in different growth stages. The experimental results can provide a reference for further research on the regulation mechanism of QDPR gene in chicken growth and development.

Key words: QDPR gene, Wumeng Crested chicken, growing development, clone, tissue expression, bioinformatics

CLC Number:

S831.2

MO Xianting, ZHANG Yong, LUO Keyin, HUANG Mingjie, WANG Tiansong, GUO Yong, YANG Hong. Cloning of QDPR Gene and Its Expression in Various Tissues in Different Growth Stages of Wumeng Crested Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1196-1206.

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Cloning of QDPR Gene and Its Expression in Various Tissues in Different Growth Stages of Wumeng Crested Chicken

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