鸡KLF3基因的表达规律及其对脂肪细胞分化的影响研究

doi:10.11843/j.issn.0366-6964.2015.01.004

Abstract

Abstract:

This experiment was conducted to study the expression pattern of Krüppel-like factor 3(Gallus gallus KLF3，gKLF3) gene，and its effect on adipocyte differentiation.The expression pattern of gKLF3 in the adipose tissue and adipocytes of broilers were analyzed by using qRT-PCR.The effect of gKLF3 on adipocyte differentiation was analyzed by over-expression technology.The result showed that gKLF3 was consecutively expressed during the chicken adipose tissue development from 2- to 10-week-old.At 10-week-old，the gKLF3 expression in the abdominal fat tissues was significantly higher in fat line chicken than that in lean line chicken(P<0.05).Additionally，the significantly higher gKLF3 expression level was observed in preadipocytes in contrast to that in mature adipocytes(P<0.01).The expression levels of gKLF3 in preadipocytes induced by oleate were lower than that in control.Moreover，over-expression of gKLF3 inhibited adipocyte differentiation and down-regulated expression of FAS and C/EBPα (P<0.01).The result indicate that gKLF3 play an important role in the growth and development of chicken abdominal adipose tissue，and might restrain adipocyte differentiation by inhibiting the expression of C/EBPα and FAS.

CLC Number:

S831.2

WANG Hai-xia,ZHANG Zhi-wei，HE Qi，WANG Ning，WANG Yu-xiang,CAO Zhi-ping，LI Hui. Expression Pattern of Chicken Krüppel-like Factor 3 Gene and Its Effect on Adipocyte Differentiation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 26-31.

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Expression Pattern of Chicken Krüppel-like Factor 3 Gene and Its Effect on Adipocyte Differentiation

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