cPouV和cNanog基因在鸡胚多能性细胞发育过程中的表达定位

doi:10.11843/j.issn.0366-6964.2014.05.008

Abstract

Abstract:

Spatio-temporal expression of cNanog and cPouV genes in the early chicken embryos was studied to provide basic information for studing functions of those two genes on chicken embryonic stem cells （CES) self-renewal and differentiation.Digoxigenin(DIG)-labeled cPouV and cNanog probes were produced by cDNA synthesization from the plasmids of pMD-18T-cPouV and pMD-18T-cNanog respectively according to a standard protocol.The expression patterns of cPouV and cNanog in the early chicken embryos were detected using in situ hybridization with the synthesized probes.The results showed that at pre-primitive streak stage，cPouV and cNanog genes were uniformly expressed in the pellucid area of embryos.At primitive streak stage，cPouV genes were strongly expressed in primitive streak and surrounding area.Hybridization signals of cNanog genes were detected only in surrounding area of primitive streak，no signals were detected inside streak.As the neural plate formed，two genes were strongly expressed in the neural plate，neuraltube and the anterior hindbrain/posterior midbrain.Later，at stage 14 HH and subsequently，two genes were strongly expressed in the head，the heart and peripheral vessels.As chicken embryo development，the pluripotent stem cells which can express cPouV and cNanog genes migrated from pellucid area to peripheral area.

CLC Number:

S831.2

LI Dong-sheng，CHEN Zhi-sheng，WANG Bing-yun，PENG Te，CHEN Sheng-feng，JI Hui-qin，CHEN Jin-ding. Expression and Localization of cPouV and cNanog Genes in the Chicken Embryo Pluripotent Cells Development[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(5): 733-739.

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Expression and Localization of cPouV and cNanog Genes in the Chicken Embryo Pluripotent Cells Development

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