唾液乳杆菌对奶山羊隐性乳房炎的治疗效果分析

doi:10.11843/j.issn.0366-6964.2024.12.033

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (12): 5706-5715.doi: 10.11843/j.issn.0366-6964.2024.12.033

唾液乳杆菌对奶山羊隐性乳房炎的治疗效果分析

朱芳¹(), 李璐璐¹(), 赵红奕¹, 董娅荣¹, 姜悦才¹, 李登亮¹, 张天亮², 熊楠楠¹, 陈德坤¹^,*(), 马文涛¹^,*(), 赵慧英¹^,*()

1. 西北农林科技大学动物医学院，杨凌 712100
2. 陕西千阳莎能奶山羊发展有限公司，宝鸡 721100

收稿日期:2023-10-31 出版日期:2024-12-23 发布日期:2024-12-27
通讯作者: 陈德坤,马文涛,赵慧英 E-mail:zhufang@nwsuaf.edu.cn;lilulu778@nwsuaf.edu.cn;cdk@nwsuaf.edu.cn;mawentao@nwsuaf.edu.cn;zhaohuiying@nwsuaf.edu.cn
作者简介:朱芳(1998-)，女，河南商丘人，硕士，主要从事奶山羊乳房炎研究，E-mail: zhufang@nwsuaf.edu.cn
李璐璐(2001-)，女，河北邯郸人，硕士，主要从事动物益生菌研究，E-mail：lilulu778@nwsuaf.edu.cn
第一联系人：
朱芳和李璐璐为同等贡献作者
基金资助:
陕西省2023年大学生创新创业训练项目(S202310712366)；中央高校基本科研业务费专项资金(2452023072)

Treatment Effects of Lactobacillus salivarius on Subclinical Mastitis in Dairy Goats

ZHU Fang¹(), LI Lulu¹(), ZHAO Hongyi¹, DONG Yarong¹, JIANG Yuecai¹, LI Dengliang¹, ZHANG Tianliang², XIONG Nannan¹, CHEN Dekun¹^,*(), MA Wentao¹^,*(), ZHAO Huiying¹^,*()

1. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China
2. Shaanxi Qianyang Shaneng Milk Goat Development Company, Baoji 721100, China

Received:2023-10-31 Online:2024-12-23 Published:2024-12-27
Contact: CHEN Dekun, MA Wentao, ZHAO Huiying E-mail:zhufang@nwsuaf.edu.cn;lilulu778@nwsuaf.edu.cn;cdk@nwsuaf.edu.cn;mawentao@nwsuaf.edu.cn;zhaohuiying@nwsuaf.edu.cn

摘要/Abstract

摘要：

旨在探究唾液乳杆菌对奶山羊隐性乳腺炎的治疗效果。本试验随机选用患有乳腺炎的奶山羊12只，其中6只作为益生菌治疗组，6只作为乳腺炎模型对照组；随机选用健康奶山羊12只，其中6只作为益生菌对照组，6只作为空白对照组。益生菌治疗组与益生菌对照组每天饲喂唾液乳杆菌，其余不作处理。在饲喂第0、28天采集奶山羊血液、粪便和乳汁，通过荧光定量PCR和16S rRNA菌群测序的方法，分别检测血清炎症因子、肠道和乳汁菌群变化。结果发现，奶山羊饲喂唾液乳杆菌后隐性乳腺炎的发病率从36.36%下降至13.64%；血液中IL-17、IL-1β和IL-6等炎性因子的相对表达量显著降低，而抗炎因子IL-10的相对表达量显著升高；肠道和乳汁中的微生物Alpha多样性升高，菌群多样性增加，在肠道微生物门水平上显著提高了厚壁菌门的相对丰度，降低了拟杆菌门的相对丰度，在乳汁微生物门水平上提高了厚壁菌门和蓝藻菌门的相对丰度，降低了变形菌门的相对丰度。综上表明，唾液乳杆菌对隐性乳腺炎具有较好的治疗效果，具有应用于隐性乳腺炎治疗的临床前景。

关键词: 奶山羊, 隐性乳腺炎, 唾液乳杆菌, 肠道菌群, 乳汁微生物

Abstract:

The aim of this study was to explore the therapeutic effect of Lactobacillus salivarius on latent mastitis of dairy goats, in this study, 12 dairy goats with mastitis were randomly selected, of which 6 were used as probiotic treatment group and 6 were used as mastitis model control group. Another twelve healthy dairy goats were selected, of which 6 were used as probiotics prevention group and 6 were used as blank control group. Probiotic treatment group and probiotic control group were fed Lactobacillus salivary every day, and the rest groups were not treated. The milk, anticoagulant and rectal contents of dairy goats were collected after feeding on day 0 and 28. The changes of blood inflammatory factors, intestinal flora and milk flora were detected by fluorescence quantitative PCR and 16S rRNA microflora sequencing, respectively. The results showed that the incidence of subclinical mastitis of dairy goats fed Lactobacillus salivary decreased from 36.36% to 13.64%, The relative expression levels of inflammatory factors such as IL-17, IL-1β and IL-6 were significantly decreased, while the relative expression levels of anti-inflammatory factor IL-10 were significantly increased. The microbial Alpha diversity increased in the gut and milk, and the diversity of flora increased, which significantly increased the relative abundance of Firmicutes and decreased the relative abundance of Bacteroidetes at the phylum level of intestinal microbiome, and increased the relative abundance of Firmicutes and Cyanobacteria at the phylum level of milk microbiome, and decreased the relative abundance of Proteobacteria.In conclusion, Lactobacillus salivarius has a good therapeutic effect on occult mastitis, and has a clinical prospect for the treatment of occult mastitis.

Key words: dairy goats, subclinical mastitis, Lactobacillus salivarius, intestinal flora, milk microorganisms

中图分类号:

S857.26

朱芳, 李璐璐, 赵红奕, 董娅荣, 姜悦才, 李登亮, 张天亮, 熊楠楠, 陈德坤, 马文涛, 赵慧英. 唾液乳杆菌对奶山羊隐性乳房炎的治疗效果分析[J]. 畜牧兽医学报, 2024, 55(12): 5706-5715.

ZHU Fang, LI Lulu, ZHAO Hongyi, DONG Yarong, JIANG Yuecai, LI Dengliang, ZHANG Tianliang, XIONG Nannan, CHEN Dekun, MA Wentao, ZHAO Huiying. Treatment Effects of Lactobacillus salivarius on Subclinical Mastitis in Dairy Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(12): 5706-5715.

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判定结果 Judgment results	性状 Characters
阴性 Negative	乳汁与诊断试剂混合后呈液体；倾斜测试盘底部无沉淀
可疑 Suspicious	乳汁与诊断试剂混合后呈液体；倾斜测试盘底部有微量沉淀
+	测试盘底部有少量沉淀；倾斜测试盘沉淀具有黏性，但不成胶状
++	测试盘底部有较多沉淀和少量胶状物；倾斜测试盘沉淀具有黏性，黏附盘底难以晃动；水平摇动测试盘，沉淀向中心聚集
+++	测试盘底部有大量明显胶状物沉淀，严重黏附盘底；水平摇动测试盘，沉淀向中心聚集，难以散开

基因名称 Gene name	序列 Sequence	物种 Species
GAPDH	F: GCGATACTCACTCTTCTACTTTCGA	Goat
GAPDH	R: TCGTACCAGGAAATGAGCTTGAC	Goat
IL-17	F: TTATCACAAGCGCTCCACCT	Goat
IL-17	R: GCACAATGGTTCTTCCAGGTT	Goat
IL-23	F: CTGAGCAGACTCCAAGCCCTAT	Goat
IL-23	R: TTGGCTCACAGGTGTCTAGGTT	Goat
IL-6	F: TCTGGGTTCAATCAGGCGAT	Goat
IL-6	R: TGTTTGTGGCTGGAGTGGTT	Goat
IL-1β	F: TCCACCTCCTCTCACAGGAAA	Goat
IL-1β	R: TACCCAAGGCCACAGGAATCT	Goat
TNF-α	F: GCACTTCGGGGTAATCGGC	Goat
TNF-α	R: GCCTTGAGGGCATTGGCAT	Goat
IL-10	F: CCACAAGTCCGACTCAACGA	Goat
IL-10	R: GTCTTCACTTTGCCGAAGGC	Goat

组别 Group	饲喂时间/d Feeding time	检出率/% Detection rate
饲喂组(益生菌治疗组+益生菌对照组)	0	36.36
Feeding group(probiotic treatment group+probiotic prevention group)	28	13.64
对照组(乳腺炎对照组+空白对照组)	0	36.11
Control group(mastitis model control group+blank control group)	28	38.89

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唾液乳杆菌对奶山羊隐性乳房炎的治疗效果分析

Treatment Effects of Lactobacillus salivarius on Subclinical Mastitis in Dairy Goats

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