增益素对溃疡性结肠炎犬免疫功能、肠道屏障和肠道菌群的影响

doi:10.11843/j.issn.0366-6964.2023.03.037

摘要/Abstract

摘要： 本试验旨在研究免疫增强剂“增益素”对溃疡性结肠炎(ulcerative colitis, UC)造模犬免疫、肠道屏障以及肠道菌群的影响。将24只10~11月龄的比格犬随机分为4组，每组6只犬。对照组和模型组饲喂基础饲粮，低剂量组和高剂量组分别在基础饲粮中添加1 000和2 000 mg·kg^-1增益素，试验第0天模型组、低剂量组和高剂量组通过直肠灌注7%冰醋酸2 mL·kg^–1建立UC模型，对照组直肠灌注相应剂量的生理盐水继续饲养7周。结果显示：1)造模24 h 后，模型组、低剂量组和高剂量组犬呈现里急后重、回头顾腹、不安、粪便不成形、带血，内窥镜检查结果显示，肠道黏膜充血水肿、有明显溃疡。2)与对照组相比，模型组血清IL-6显著升高(P<0.05)，IL-10极显著降低(P<0.01)，而低剂量组和高剂量组血清IL-6和IL-10均回到正常水平。此外，与模型组相比，低剂量组和高剂量组的粪便SIgA含量也显著提高(P<0.05)。3)试验第49天，与对照组相比，模型组血清LPS水平显著升高(P<0.05)；与模型组相比，低剂量组的血清LPS水平及高剂量组的血清二胺氧化酶(DAO)含量均显著降低(P<0.05)。4)与对照组相比，模型组、低剂量组、高剂量组的肠道菌群结构均差异显著(P<0.05)，模型组毛螺菌(Lachnospiraceae)的相对丰度显著增加；与模型组比，低剂量组菌群结构差异不显著(P>0.05)，高剂量组菌群结构差异极显著(P<0.01)，其中，低剂量组Ruminiclostridium显著增加(P<0.05)，高剂量组巨型球菌(Megasphaera)显著增加(P<0.05)。综上，犬粮中添加增益素可提高UC造模犬免疫功能、改善其肠道物理屏障功能及菌群结构，推荐在犬粮中添加2 000 mg·kg^–1增益素。

关键词: 增益素, 犬溃疡性结肠炎, 免疫功能, 肠道屏障, 肠道菌群

Abstract: This study was aimed to evaluate the effects of the immune regulator “polyactin A” on immune function, intestinal barrier and intestinal flora in dogs modeled for ulcerative colitis (UC). Twenty-four 10- to 11-month-old Beagle dogs were randomly divided into four groups, each group contained six dogs. The Control group and the Model group were fed the basal diet, and the Low-dose group and the High-dose group were added 1 000 and 2 000 mg·kg^-1 of polyactin A to the basal diet, respectively. The Model, Low-dose and High-dose groups were established by rectal instillation of 7% glacial acetic acid at 2 mL·kg^-1 for UC model on day 0 of the experiment, The Control group was rectal instilled with the same dose of saline and continued for be fed 7 weeks. The results showed that: 1) The dogs in the Model group, Low-dose group and High-dose group showed posterior urgency, turning back to look at the abdomen, restlessness, unformed and bloody stools, and endoscopic findings showed congested and edematous intestinal mucosa with obvious ulcers 24 h after modeling. 2) Compared with the Control group, serum IL-6 was significantly higher (P<0.05) and IL-10 was highly significantly lower (P<0.01) in the Model group, while serum IL-6 and IL-10 returned to normal levels in both the Low-dose and High-dose groups. In addition, the fecal SIgA levels were also significantly higher in the Low-dose and High-dose groups compared with the Model group (P<0.05). 3) On day 49 of the test, serum LPS levels were significantly higher in the model group compared with the Control group (P<0.05); serum LPS levels in the Low-dose group and serum DAO levels in the High-dose group were significantly lower compared with the Model group (P<0.05). 4) Compared with the Control group, the intestinal flora structure of the Model, Low-dose and High-dose groups all differed significantly (P<0.05), and the relative abundance of Lachnospiraceae in the Model group increased significantly; compared with the Model group, the difference in the flora structure of the Low-dose group was not significant (P>0.05), and the difference in the flora structure of the High-dose group was highly significant (P<0.01), with Ruminiclostridium was significantly increased in the Low-dose group (P<0.05) and Megasphaera was significantly increased in the High-dose group (P<0.05). In conclusion, the addition of polyactin A to dog food can improve the immune function, the physical barrier function and the flora structure of the intestinal tract in UC-modeled dogs, and the addition of 0.2% polyactin A to dog food is recommended.

Key words: polyactin A, caine ulcerative colitis, immune function, intestinal barrier, intestinal flora

中图分类号:

S856.4

汪倩, 王建梅, 安柯颖, 夏兆飞. 增益素对溃疡性结肠炎犬免疫功能、肠道屏障和肠道菌群的影响[J]. 畜牧兽医学报, 2023, 54(3): 1261-1272.

WANG Qian, WANG Jianmei, AN Keying, XIA Zhaofei. Effect of Polyactin A on Immune Function, Intestinal Barrier and Intestinal Flora in Dogs with Ulcerative Colitis[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1261-1272.

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