饲粮中添加芦丁对肉鸡回肠形态、免疫、抗氧化及屏障功能的影响

doi:10.11843/j.issn.0366-6964.2023.02.020

摘要/Abstract

摘要： 本试验旨在研究芦丁（rutin）对肉鸡回肠组织形态、免疫、抗氧化及屏障功能的影响。选择256只1日龄AA肉鸡，随机均分为4组，每组8个重复，每个重复8只，分别饲喂基础饲粮添加0（对照组）、250、500和1 000 mg·kg^-1的芦丁。试验分为前期（1~21 d）和后期（22~42 d），试验期42 d。结果显示：1）饲粮中添加芦丁二次曲线提高21 d肉鸡回肠黏膜Bcl-2和ZO-1 mRNA的表达量（P_Q<0.05），线性增加42 d回肠绒毛高度（VH）以及回肠黏膜中Bcl-2、ZO-1和Mucin2 mRNA的表达量（P_L<0.05），且添加500 mg·kg^-1芦丁显著提高了42 d回肠VH及黏膜中ki67 mRNA的表达量（P<0.05）。2）饲粮中添加芦丁线性和二次曲线增加21 d回肠黏膜中分泌性免疫球蛋白（sIgA）含量（P_L<0.05；P_Q<0.05），降低42 d回肠黏膜中白细胞介素2（IL-2） mRNA的表达量（P_L<0.05；P_Q<0.05），并线性降低肿瘤坏死因子-α（TNF-α） mRNA的表达量（P_L<0.05）。同时，与对照组相比，添加250和500 mg·kg^-1芦丁显著降低了21 d回肠黏膜中核因子-κB （NF-κB） mRNA的表达量（P<0.05），添加500 mg·kg^-1芦丁显著降低了42 d NF-κB mRNA的表达量（P<0.05）。3）21 d时，饲粮中添加芦丁线性和二次曲线提高了回肠黏膜中T-AOC （P_L<0.05；P_Q<0.05）和GSH-Px活性（P_L=0.096；P_Q<0.05）以及HO-1 mRNA的表达量（P_L<0.05；P_Q<0.05），线性增加NQO1 mRNA的表达量（P_L<0.05），并二次曲线降低回肠黏膜中MDA含量（P_Q<0.05），提高Nrf2 mRNA的表达量（P_Q<0.05）；与对照组相比，芦丁处理组显著提高回肠黏膜中HO-1 mRNA的表达量（P<0.05），250和500 mg·kg^-1芦丁组显著降低回肠黏膜中MDA含量（P<0.05），提高GSH-Px活性（P<0.05），500 mg·kg^-1芦丁组回肠黏膜中T-AOC活性和NQO1 mRNA的表达量也显著高于对照组（P<0.05）。42 d时，饲粮中添加芦丁线性和二次曲线降低回肠黏膜中MDA含量（P_L<0.05；P_Q<0.05），Nrf2和HO-1 mRNA的表达量呈二次曲线增加（P_Q<0.05），与对照组相比，添加500 mg·kg^-1芦丁显著降低了回肠黏膜中MDA含量（P<0.05），提高T-SOD活性和Nrf2、HO-1 mRNA的表达量（P<0.05）。综上，饲粮添加芦丁能够改善肉鸡回肠形态结构、提高肠道免疫和屏障功能并通过增强回肠组织中Nrf2信号通路提高其抗氧化能力。在本试验条件下，综合芦丁对肉鸡全期的作用效果，推荐芦丁添加量为500 mg·kg^-1。

关键词: 芦丁, 肉鸡, 抗氧化, 免疫, 肠道屏障, 肠道功能

Abstract: The purpose of this experiment was to study the effect of rutin on the ileal tissue morphology, immunity, antioxidant and barrier function of broilers. A total of 256 1-day-old AA broilers were selected and randomly divided into 4 groups, with 8 replicates in each group and 8 chickens in each replicate, and were fed the basal diet supplemented with 0 (control group), 250, 500 and 1 000 mg·kg^-1of rutin, respectively. The experiment was divided into early stage (1-21 d) and late stage (22-42 d), and the experiment lasted for 42 days. The results were showed as follows:1) Dietary rutin significantly increased the mRNA expression levels of Bcl-2 and ZO-1 in ileum mucosa of broilers at 21 d (P_Q<0.05), and linearly increased the villus height (VH) of ileum and the mRNA expression levels of Bcl-2, ZO-1 and Mucin2 in ileum mucosa of broilers at 42 d (P_L<0.05). Moreover, 500 mg·kg^-1 rutin supplementation significantly increased the expression of ki67 mRNA in ileal mucosa and the ileal VH at 42 d (P<0.05). 2) The addition of rutin to the diet linearly and quadratic increased the content of secreted immunoglobulin (sIgA) in the ileal mucosa at 21 d (P_L<0.05; P_Q<0.05), and decreased the mRNA expression of interleukin-2 (IL-2) in ileum mucosa at 42 d (P_L<0.05; P_Q<0.05), also linearly decreased the mRNA expression of tumor necrosis factor -α (TNF-α) (P_L<0.05). At the same time, compared with the control group, the addition of 250 and 500 mg·kg^-1 rutin decreased the expression of nuclear factor-κB (NF-κB) mRNA in the ileal mucosa at 21 d, while the addition of 500 mg·kg^-1 rutin decreased the mRNA expression level of NF-κB in ileal mucosa at 42 d (P<0.05). 3) At 21 d, dietary rutin supplementation increased the T-AOC in ileum mucosa linearly and quadratic (P_L<0.05; P_Q<0.05), as well as GSH-Px activity (P_L=0.096;P_Q<0.05) and HO-1 mRNA expression (P_L<0.05; P_Q<0.05). Also linearly increased the expression level of NQO1 mRNA (P_L<0.05), quadratic decreased the content of MDA in ileum mucosa (P_Q<0.05), and increased the expression level of Nrf2 mRNA (P_Q<0.05); Compared with control group, the expression level of HO-1 mRNA in ileum mucosa in rutin treatment group were significantly increased (P<0.05), the content of MDA in ileum mucosa in 250 and 500 mg·kg^-1 rutin groups were significantly decreased (P<0.05), and the activity of GSH-Px in ileum mucosa was increased (P<0.05). T-AOC and the mRNA expression of NQO1 in ileum mucosa of 500 mg·kg^-1rutin group were also significantly higher than those in control group (P<0.05). At 42 d, the supplementation of rutin linearly and quadratic decreased the content of MDA in ileum mucosa (P_L<0.05; P_Q<0.05), the mRNA expression levels of Nrf2 and HO-1 increased quadratic (P_Q<0.05), and compared with the control group, the supplementation of 500 mg·kg^-1 rutin significantly decreased the content of MDA in ileum mucosa (P<0.05). The activity of T-SOD and mRNA expression levels of Nrf2 and HO-1 were increased (P<0.05). In summary, supplementation of rutin in the diet could improve the ileal morphology and structure of broiler chickens, improve intestinal immunity and barrier function, and improve its antioxidant capacity by enhancing the Nrf2 signaling pathway in ileal tissue. Under the conditions of this experiment, considering the effect of rutin on the whole period of broilers, the recommended amount of rutin in the diet was 500 mg·kg^-1.

Key words: rutin, broiler, antioxidant, immunity, intestinal barrier, intestinal function

中图分类号:

S831.5

刘慧娟, 王超, 周斌斌, 张佳琦, 王恬, 庄苏. 饲粮中添加芦丁对肉鸡回肠形态、免疫、抗氧化及屏障功能的影响[J]. 畜牧兽医学报, 2023, 54(2): 630-641.

LIU Huijuan, WANG Chao, ZHOU Binbin, ZHANG Jiaqi, WANG Tian, ZHUANG Su. Effects of Dietary Rutin Supplementation on Ileal Morphology, Immunity, Antioxidant and Barrier Function of Broilers[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 630-641.

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