一株鸭腺病毒3型的分离、鉴定及致病性分析

doi:10.11843/j.issn.0366-6964.2023.05.026

摘要/Abstract

摘要： 从现地分离一株3型鸭腺病毒(duck adenovirus 3,DAdV-3)并命名为DAdV-3 YN株,分析该毒株的基因特征与致病特性,本研究选用LMH细胞进行病毒分离与纯化,并对YN株的hexon、penton base、fiber1和fiber2基因进行测序分析。通过腿部肌肉注射病毒感染7日龄番鸭,观察番鸭精神状态,记录体重变化、脏器剖检特征、组织病理学变化、计算脏器载毒量、泄殖腔拭子排毒量以及血清生化等数据,同时测定法氏囊与脾内凋亡相关基因转录水平以探究YN株对宿主免疫功能的影响。结果显示,番鸭感染YN株后体重增长受到显著抑制(P＜0.01; P＜0.000 1);体内各脏器均出现不同程度病变,攻毒组鸭的肝组织与肾组织脏器系数均大于对照组且在攻毒后第7天差异极显著(P＜0.01);攻毒组番鸭血清内丙氨酸氨基转移酶、天冬氨酸氨基转移酶和尿素氮含量均显著高于对照组(P＜0.001,P＜0.000 1);同时,法氏囊与脾内细胞凋亡相关基因mRNA水平显著上升(P＜0.01; P＜0.001)。以上结果提示,YN株感染7日龄番鸭后,可以导致肝和肾等脏器损伤,同时可以诱导法氏囊与脾内细胞凋亡从而影响番鸭免疫功能。

关键词: 鸭腺病毒3型, 基因特征, 致病力, 免疫功能

Abstract: The purpose of this study was to isolate a novel Duck adenovirus 3 (DAdV-3) YN strain and elucidate its genetic characteristics and pathogenicity. LMH cells were selected for virus isolation and purification, and then the virus genes, including hexon, penton base, fiber1, and fiber2 were sequenced and analyzed. Seven-days-old Muscovy ducks were infected by intramuscular injection in the legs, and the mental state of the ducks was observed. Besides, the body weight changes, necropsy characteristics of organs, histopathological changes, viral load of organs, cloaca swab detoxification, and serum biochemistry were recorded and analyzed. Furthermore, the transcription levels of apoptosis-related genes in the bursa and the spleen were measured to explore the effect of YN strain on immune function. The results showed that the weight gain of infected ducks was significantly inhibited (P<0.01, P<0.000 1), and different degrees of lesions appeared in various organs. The coefficients of livers and kidneys of the experimental group were larger than those of the control group, and the difference was extremely significant on the 7th day after the challenge. Alanine aminotransferase, aspartate aminotransferase, and urea nitrogen in the serum of the Muscovy ducks in the challenge group were significantly higher than those in the control group (P<0.001, P<0.0001). At the same time, the mRNA levels of apoptosis-related genes in the bursa and the spleen increased significantly (P<0.01, P<0.001). (Conclusion) The above results suggest that DAdV-3 YN strain can damage livers and kidneys when challenging the 7-days-old Muscovy ducks, simultaneously, it can affect the immune function of Muscovy ducks by causing apoptosis of cells in the bursa and the spleen.

Key words: duck adenovirus 3, genetic characteristics, pathogenicity, immune function

中图分类号:

S858.32

曹秀芸, 刘吉文, 汤智辉, 郑紫怡, 闫丽萍, 宋素泉. 一株鸭腺病毒3型的分离、鉴定及致病性分析[J]. 畜牧兽医学报, 2023, 54(5): 2050-2061.

CAO Xiuyun, LIU Jiwen, TANG Zhihui, ZHENG Ziyi, YAN Liping, SONG Suquan. Isolation, Identification and Pathogenicity Analysis of a Duck Adenovirus Type 3[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2050-2061.

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