三氧化二砷对鸡肝脏氧化应激和蛋氨酸亚砜还原酶表达的影响

doi:10.11843/j.issn.0366-6964.2020.11.025

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (11): 2867-2874.doi: 10.11843/j.issn.0366-6964.2020.11.025

三氧化二砷对鸡肝脏氧化应激和蛋氨酸亚砜还原酶表达的影响

伍少峰^1,2, 钟高龙¹, 万方¹, 宁芷君¹, 蒋璇璇¹, 胡婷¹, 陈忠伟², 赵武², 卢冰霞², 唐兆新¹, 何颖^2*, 胡莲美^1*

1. 华南农业大学兽医学院, 广州 510642;
2. 广西壮族自治区兽医研究所广西兽医生物技术重点实验室, 南宁 530001

收稿日期:2020-05-25 出版日期:2020-11-25 发布日期:2020-11-20
通讯作者: 何颖,主要从事动物传染病与分子生物学研究,E-mail:yinghe2@gmail.com;胡莲美,主要从事畜禽营养代谢病和中毒病的教学与研究,E-mail:hulianmei@scau.edu.cn
作者简介:伍少峰(1995-),男,土家族,湖南张家界人,硕士生,主要从事中毒与营养代谢病研究,E-mail:2639271703@qq.com
基金资助:
国家自然科学基金（31402264）；广西创新驱动发展专项（桂科AA17204057-1；桂科AA18118051）；柳州市科学研究与技术开发计划项目（2018BH20501）；广西南宁市西乡塘区科学研究与技术开发计划项目（201810204）；玉林市科学研究与技术开发计划项目（玉市科201934022）

Effects of Arsenic Trioxide Treatment on Oxidative Stress and Methionine Sulfoxide Reductase Expression in Chicken Liver

WU Shaofeng^1,2, ZHONG Gaolong¹, WAN Fang¹, NING Zhijun¹, JIANG Xuanxuan¹, HU Ting¹, CHEN Zhongwei², ZHAO Wu², LU Bingxia², TANG Zhaoxin¹, HE Ying^2*, HU Lianmei^1*

1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
2. Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China

Received:2020-05-25 Online:2020-11-25 Published:2020-11-20

摘要/Abstract

摘要： 本研究旨在探讨三氧化二砷（ATO）对鸡肝脏氧化应激和蛋氨酸亚砜还原酶（Msrs）表达的影响。将32只1日龄雏鸡分为对照组（生理盐水）、低剂量组（1 mg·kg^-1 ATO溶液）、中剂量组（3 mg·kg^-1 ATO溶液）和高剂量组（9 mg·kg^-1ATO溶液），用相应浓度的ATO溶液通过灌胃处理雏鸡，每天灌胃1次，持续5周。试验期结束后，取肝组织并分析肝脏系数和观察肝组织病理变化；测定肝组织中MDA水平和SOD活力；检测Msrs基因和蛋白的表达水平。结果显示，与对照组相比，中、高剂量组肝脏系数极显著升高（P<0.01），中、高剂量组肝有淤血和水泡变性；相比于对照组，试验组MDA水平极显著增加（P<0.01），SOD活力极显著下降（P<0.01）；低、中、高剂量组中MsrA、MsrB1、MsrB3基因mRNA表达水平相比对照组极显著增加（P<0.01或P<0.001），但MsrA蛋白水平极显著降低（P<0.01）。结果表明，ATO诱导鸡肝发生氧化应激，同时通过促进Msrs表达以清除ROS，减轻肝氧化损伤。

关键词: 三氧化二砷, Msrs, 鸡, 肝, 氧化应激

Abstract: This study aimed to investigate the effect of arsenic trioxide (ATO) on oxidative stress and methionine sulfoxide reductase (Msrs) expression in chicken liver. A total of 32 one-day-old chicks were divided into control group (saline solution), low-dose group (1 mg·kg^-1 ATO solution), medium-dose group (3 mg·kg^-1 ATO solution) and high-dose group (9 mg·kg^-1 ATO solution). Chickens were treated by gavage with ATO solution of corresponding concentration once a day for 5 weeks. The liver coefficient, histopathological sections, MDA level, SOD activity, Msrs gene and protein expression levels of liver were analyzed. The results showed that compared with the control group, the liver coefficient of the medium- and high-dose groups were significantly increased (P<0.01), and the liver congestion and vesicular degeneration were found in the medium- and high-dose groups. Meanwhile, the experimental groups had significantly increased MDA levels (P<0.01) and decreased SOD activity (P<0.01) as compared with the control group; MsrA, MsrB1, MsrB3 gene mRNA expression levels in the ATO treatment groups were significantly increased compared with the control group (P<0.01 or P<0.001); However, the protein levels of MsrA were reduced significantly (P<0.01). The results indicated that ATO induced oxidative stress in the liver of chickens, and at the same time promoted the expression of Msrs in liver to clear ROS and reduce liver oxidative damage.

Key words: arsenic trioxide, Msrs, chicken, liver, oxidative stress

中图分类号:

S856.9

伍少峰, 钟高龙, 万方, 宁芷君, 蒋璇璇, 胡婷, 陈忠伟, 赵武, 卢冰霞, 唐兆新, 何颖, 胡莲美. 三氧化二砷对鸡肝脏氧化应激和蛋氨酸亚砜还原酶表达的影响[J]. 畜牧兽医学报, 2020, 51(11): 2867-2874.

WU Shaofeng, ZHONG Gaolong, WAN Fang, NING Zhijun, JIANG Xuanxuan, HU Ting, CHEN Zhongwei, ZHAO Wu, LU Bingxia, TANG Zhaoxin, HE Ying, HU Lianmei. Effects of Arsenic Trioxide Treatment on Oxidative Stress and Methionine Sulfoxide Reductase Expression in Chicken Liver[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(11): 2867-2874.

参考文献 38

[1]	罗婷,孙健雄,夏科,等.土壤砷污染研究综述[J].环境与发展,2017,29(8):11-12.LUO T,SUN J X,XIA K,et al.A research review of arsenic pollution in soil[J].Inner Mongolia Environmental Sciences,2017,29(8):11-12.(in Chinese)
[2]	BJØRKLUND G,OLIINYK P,LYSIUK R,et al.Arsenic intoxication:general aspects and chelating agents[J].Arch Toxicol,2020,94(6):1879-1897.
[3]	袁慧,陈竞峰,向建洲,等.畜禽配合饲料中砷的污染量及其分析报告[J].湖南饲料,2000(3):2-3.YUAN H,CHEN J F,XIANG J Z,et al.Pollution amount of arsenic in livestock and poultry compound feed and its analysis report[J].Hunan Feed,2000(3):2-3.(in Chinese)
[4]	游玉萍,岳伟,张小琴,等.砷铜合金中铜的电解测定[J].南方金属,2008(6):12-13.YOU Y P,YUE W,ZHANG X Q,et al.Determination of copper in copper-arsenic alloys by electrolytic gravimetry[J].Southern Metals,2008(6):12-13.(in Chinese)
[5]	CAI X N,YU L L,CHEN Z,et al.Arsenic trioxide-induced upregulation of miR-1294 suppresses tumor growth in hepatocellular carcinoma by targeting TEAD1 and PIM1[J].Cancer Biomark,2020,28(2):221-230.
[6]	COCHET C,SIMONET M,CATTIN J,et al.Arsenic trioxide treatment during pregnancy for acute promyelocytic leukemia in a 22-year-old woman[J].Case Rep Hematol,2020,2020:3686584.
[7]	褚海义,闫贵龙,岳春旺,等.三氧化二砷促进肥育猪生长的研究[J].河北畜牧兽医,2001,17(2):14-15.CHU H Y,YAN G L,YUE C W,et al.Study on the promotion of arsenic trioxide to the growth of finishing pigs[J].Hebei Animal Husbandry and Veterinary Medicine,2001,17(2):14-15.(in Chinese)
[8]	OMMATI M M,HEIDARI R,MANTHARI R K,et al.Paternal exposure to arsenic resulted in oxidative stress,autophagy,and mitochondrial impairments in the HPG axis of pubertal male offspring[J].Chemosphere,2019,236:124325.
[9]	李文珍,杨洪莉.浅谈砷污染[J].环境保护科学,1995,21(3):57,67.LI W Z,YANG H L.Review on arsenic pollution[J].Environmental Protection Science,1995,21(3):57,67.(in Chinese)
[10]	ZENG Q,YI H L,HUANG L Q,et al.Long-term arsenite exposure induces testicular toxicity by redox imbalance,G2/M cell arrest and apoptosis in mice[J].Toxicology,2019,411:122-132.
[11]	NGO T C,MAI T V T,PHAM T T,et al.Natural acridones and coumarins as free radical scavengers:mechanistic and kinetic studies[J].Chemical Physics Letters,2020,746:137312.
[12]	李灵珺,经鸿宇,彭西,等.蛋氨酸缺乏对雏鸡肝细胞周期的影响[J].西华师范大学学报:自然科学版,2020,41(1):17-22.LI L J,JING H Y,PENG X,et al.Effect of methionine deficiency on hepatic cell cycle of broiler chicken[J].Journal of China West Normal University:Natural Sciences,2020,41(1):17-22.(in Chinese)
[13]	REITERER M,SCHMIDT-KASTNER R,MILTON S L.Methionine sulfoxide reductase (Msr) dysfunction in human brain disease[J].Free Radic Res,2019,53(11-12):1144-1154.
[14]	LEE B C,DIKIY A,KIM H Y,et al.Functions and evolution of selenoprotein methionine sulfoxide reductases[J].Biochim Biophys Acta Gen Subj,2009,1790(11):1471-1477.
[15]	ZHONG G L,WAN F,YAN H, et al.Methionine sulfoxide reductases are related to arsenic trioxide-induced oxidative stress in mouse liver[J].Biol Trace Elem Res,2020,195(2):535-543.
[16]	PRENTICE H M,MOENCH I A,RICKAWAY Z T,et al.MsrA protects cardiac myocytes against hypoxia/reoxygenation induced cell death[J].Biochem Biophys Res Commun,2008,366(3):775-778.
[17]	KIM K Y,KWAK G H,SINGH M P,et al.Selenoprotein MsrB1 deficiency exacerbates acetaminophen-induced hepatotoxicity via increased oxidative damage[J].Arch Biochem Biophys,2017,634:69-75.
[18]	SHI T R,YANG Y J,ZHANG Z H,et al.Loss of MsrB1 perturbs spatial learning and long-term potentiation/long-term depression in mice[J].Neurobiol Learn Mem,2019,166:107104.
[19]	KWAK G H,KIM H Y.MsrB3 deficiency induces cancer cell apoptosis through p53-independent and ER stress-dependent pathways[J].Arch Biochem Biophys,2017,621:1-5.
[20]	BULVIK B E,BERENSHTEIN E,KONIJN A M et al.Aging is an organ-specific process:changes in homeostasis of iron and redox proteins in the rat[J].AGE(Dordr),2012,34(3):693-704.
[21]	ACHILLI C,CIANA A,ROSSI A, et al.Neutrophil granulocytes uniquely express,among human blood cells,high levels of Methionine-sulfoxide-reductase enzymes[J].J Leukoc Biol,2008,83(1):181-189.
[22]	CABREIRO F,PICOT C R,FRIGUET B,et al.Methionine sulfoxide reductases:relevance to aging and protection against oxidative stress[J].Ann N Y Acad Sci,2006,1067(1):37-44.
[23]	DE LUCA A,SACCHETTA P,NIEDDU M,et al.Important roles of multiple Sp1 binding sites and epigenetic modifications in the regulation of the methionine sulfoxide reductase B1(MsrB1) promoter[J].BMC Mol Biol,2007,8:39.
[24]	高婷,王子旭,陈祝茗,等.ROS介导的氧化应激与自噬[J].中国畜牧兽医,2018,45(3):656-662.GAO T,WANG Z X,CHEN Z M,et al.Oxidative stress and autophagy mediated by Reactive Oxygen Species[J].China Animal Husbandry & Veterinary Medicine,2018,45(3):656-662.(in Chinese)
[25]	XU T T,LI H,DAI Z,et al.Spermidine and spermine delay brain aging by inducing autophagy in SAMP8 mice[J].Aging (Albany NY),2020,12(7):6401-6414.
[26]	ARUOMA O I.Free radicals,oxidative stress,and antioxidants in human health and disease[J].J Am Oil Chem Soc,1998,75(2):199-212.
[27]	MU M Y,ZHAO H J,WANG Y,et al.Arsenic trioxide or/and copper sulfate co-exposure induce glandular stomach of chicken injury via destruction of the mitochondrial dynamics and activation of apoptosis as well as autophagy[J].Ecotoxicol Environ Saf,2019,185:109678.
[28]	SARKAR S,MUKHERJEE S,CHATTOPADHYAY A,et al.Differential modulation of cellular antioxidant status in zebrafish liver and kidney exposed to low dose arsenic trioxide[J].Ecotoxicol Environ Saf,2017,135:173-182.
[29]	CHEN H M,LIU G Y,QIAO N,et al.Toxic effects of arsenic trioxide on spermatogonia are associated with oxidative stress,mitochondrial dysfunction,autophagy and metabolomic alterations[J].Ecotoxicol Environ Saf,2020,190:110063.
[30]	王丛丛,孙叶娴,宁芷君,等.三氧化二砷对肉鸡原代肝细胞氧化应激和甲硫氨酸亚砜还原酶基因表达的影响[J].中国兽医科学,2018,48(3):379-385.WANG C C,SUN Y X,NING Z J,et al.Effects of arsenic trioxide on the oxidative stress and expression of methionine sulfoxide reductases' genes in chicken primary hepatocytes[J].Chinese Veterinary Science,2018,48(3):379-385.(in Chinese)
[31]	ALQUDAH S,CHERTOFF M,DURHAM D,et al.Methionine sulfoxide reductase a knockout mice show progressive hearing loss and sensitivity to acoustic trauma[J].Audiol Neurootol,2018,23:20-31.
[32]	RUAN D,FOUAD A M,FAN Q L,et al.Effects of dietary methionine on productivity,reproductive performance,antioxidant capacity,ovalbumin and antioxidant-related gene expression in laying duck breeders[J].Br J Nutr,2018,119(2):121-130.
[33]	KIM H Y.The methionine sulfoxide reduction system:selenium utilization and methionine sulfoxide reductase enzymes and their functions[J].Antioxid Redox Signal,2013,19(9):958-969.
[34]	TARRAGO L,OHEIX E,PÉTERFI Z,et al.Monitoring of methionine sulfoxide content and methionine sulfoxide reductase activity[J].Methods Mol Biol,2018,1661:285-299.
[35]	TARRAGO L,GLADYSHEV V N.Recharging oxidative protein repair:catalysis by methionine sulfoxide reductases towards their amino acid,protein,and model substrates[J].Biochemistry (Mosc),2012,77(10):1097-1107.
[36]	ZHANG C,JIA P P,JIA Y Y,et al.Anoxia,acidosis,and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells[J].J Cell Biochem,2011,112(1):98-106.
[37]	MOSKOVITZ J,STADTMAN E R.Selenium-deficient diet enhances protein oxidation and affects methionine sulfoxide reductase (MsrB) protein level in certain mouse tissues[J].Proc Natl Acad Sci U S A,2003,100(13):7486-7490.
[38]	MESHKIBAF M H,MALEKNIA M,NOROOZI S.Effect of curcumin on gene expression and protein level of methionine sulfoxide reductase A (MSRA),SOD,CAT and GPx in Freund's adjuvant inflammation-induced male rats[J].J Inflamm Res,2019,12:241-249.

三氧化二砷对鸡肝脏氧化应激和蛋氨酸亚砜还原酶表达的影响

Effects of Arsenic Trioxide Treatment on Oxidative Stress and Methionine Sulfoxide Reductase Expression in Chicken Liver

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献 38

相关文章 15

编辑推荐

Metrics

本文评价

[1]	郭妍婷, 周建民, 王晶, 齐广海. 产蛋鸡子宫部钙离子转运及调控的研究进展[J]. 畜牧兽医学报, 2024, 55(1): 39-50.
[2]	唐鑫鑫, 郑炬梅, 骆娜, 营凡, 朱丹, 李森, 刘大伟, 安炳星, 文杰, 赵桂苹, 李和刚. 基于全基因组关联分析揭示肉鸡腿病发生的遗传机制[J]. 畜牧兽医学报, 2024, 55(1): 99-109.
[3]	卢建, 张欣, 江栋材, 马猛, 王强, 王星果, 李永峰, 郭军, 窦套存, 胡玉萍, 李尚民, 邵丹, 曲亮. 甘氨酸锰对产蛋后期蛋鸡产蛋性能和肠道微生物的影响[J]. 畜牧兽医学报, 2024, 55(1): 218-231.
[4]	张德安, 杨若渚, 刘杰, 刘德武, 邓铭, 柳广斌, 孙宝丽, 郭勇庆, 李耀坤. 饲喂青贮黄梁木代替青贮玉米川中黑山羊肝转录组的表达分析[J]. 畜牧兽医学报, 2024, 55(1): 232-244.
[5]	陈玥彤, 刘晓涵, 王芷洋, 赵宇馨, 周铁忠, 胡增金, 朱悦, 王少辉, 田明星, 丁思羽, 祁晶晶, 于圣青. 广东规模化鸡场死鸡胚中鸡毒支原体的分离鉴定、致病性及药物敏感性[J]. 畜牧兽医学报, 2024, 55(1): 290-299.
[6]	马亚军, 焦智慧, 刘笑凝, 陆翔羽, 刘涛, 王月, 朴晨曦, 王洪斌. 脂肪间充质干细胞对小型猪肝缺血再灌注合并肝切除组织细胞焦亡的影响[J]. 畜牧兽医学报, 2024, 55(1): 355-364.
[7]	茹盟, 曾文惠, 彭剑玲, 曾庆节, 殷超, 崔勇, 魏庆, 梁海平, 谢贤华, 黄建珍. 蛋鸡卵泡发育及其表观遗传调控机制研究进展[J]. 畜牧兽医学报, 2023, 54(9): 3613-3622.
[8]	韩坤良, 兰伟, 胡新, 崔亚东, 孔祥峰. 复方中药超微粉对蛋鸡抗氧化性能及相关基因表达的影响[J]. 畜牧兽医学报, 2023, 54(9): 3784-3792.
[9]	张亚峰, 朱斌, 马畅, 张源淑. 二脒那秦激活ACE2对非酒精性脂肪肝病大鼠肝线粒体影响研究[J]. 畜牧兽医学报, 2023, 54(9): 3895-3904.
[10]	袁何玲, 方词, 黄金虎, 王晓明, 肖文浚, 刘睿婷, 史荣梅, 王丽平. 大蒜辣素对肉鸡肝组织中CYP1A2 mRNA表达及其酶动力学的影响[J]. 畜牧兽医学报, 2023, 54(9): 3905-3915.
[11]	陈鸿, 阮红日, 马天文, 李亚楠, 苗雪, 杨雯越, 高利, 魏成威. 葛根素干预软骨氧化应激和Nrf2/HO-1通路改善PTOA大鼠软骨退变的机制[J]. 畜牧兽医学报, 2023, 54(9): 3951-3963.
[12]	郑钢, 连玲. 鸡性别决定及分化关键调控基因DMRT1研究进展[J]. 畜牧兽医学报, 2023, 54(8): 3152-3163.
[13]	白露, 王梦杰, 马小春, 何政肖, 谭晓冬, 刘杰, 赵桂苹, 文杰, 刘冉冉. 一种快速挖掘鸡品种特征性SNP标记集合的方法[J]. 畜牧兽医学报, 2023, 54(8): 3252-3261.
[14]	陈春, 康昭风, 魏岳, 黎观红, 武艳平, 谢金防. Wnt3a基因多态性与崇仁麻鸡皮肤毛囊性状相关性研究[J]. 畜牧兽医学报, 2023, 54(7): 2810-2823.
[15]	付曦瑶, 陈丽红, 陈小丽, 孙伟丽, 郭肖兰. 11~17周龄雌性雉鸡蛋白质适宜需要量研究[J]. 畜牧兽医学报, 2023, 54(7): 2910-2923.