动物胚胎育种及应用中的技术策略

doi:doi: 10.11843/j.issn.0366-6964.2016.10.002

摘要/Abstract

摘要：

随着动物分子育种及动物胚胎工程技术的发展，生产、选择具有预期基因型的胚胎，已经成为现实。动物胚胎育种或动物胚胎工程技术育种将成为动物育种中一个重要的技术途径和手段。动物胚胎育种研究主要包括：（1）动物育种方案中胚胎工程技术的应用及应用中的技术选择和优化；（2）不同类型动物胚胎育种体系的建立及育种方案实施过程中防止群体近交等问题的研究；（3）动物胚胎育种遗传进展和生物安全等方面的科学评估。动物胚胎育种在应用方面的技术策略可归纳为几个方面：以扩繁为策略的胚胎育种；以转基因或基因修饰为策略的新品种创制育种；以品种种质资源保存和利用为策略的动物胚胎保种。动物胚胎育种是对传统动物育种理论的发展和完善，也是胚胎工程技术应用于畜牧生产中的体现，在加快动物遗传改良、本品种选育以及新品种培育等方面具有广阔的应用前景。

Abstract:

With the development of animal molecular reproduction and embryo engineering technology，the production of genetically superior offspring has become a reality.The embryo engineering technology of animal embryo reproduction has been becoming an important technical approach in animal reproduction fields.The studies of animal embryo breeding include：1) the selection and optimization of embryo engineering technology in animal breeding program；2) the establishment of different systems of animal embryo breeding and the solution exploration of the issues of animal breeding such as inbreeding avoidance；3) the scientific assessment of genetic progress and biosafety in animal embryo breeding.The applications of these technological strategies in animal embryo breeding can be summarized as following：the purebred breeding through expanding group of outstanding individuals；the breeding of new varieties by transgene or gene modification technique；the breeding conservation by using animal germplasm resources.The technology of animal embryo breeding is the development and improvement of the traditional animal breeding theories，as well as the reflection of animal embryo biotechnology application in real animal production.This animal embryo breeding technology has broad application prospects in accelerating the development of animal breeding，the breeding selection，and cultivating new varieties.

中图分类号:

S814

李延鹤，刘军，张涌，权富生. 动物胚胎育种及应用中的技术策略[J]. 畜牧兽医学报, 2016, 47(10): 1954-1960.

LI Yan-he，LIU Jun，ZHANG Yong，QUAN Fu-sheng. Technical Strategies of Animal Embryo Breeding and Its Application[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 1954-1960.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/doi: 10.11843/j.issn.0366-6964.2016.10.002

https://www.xmsyxb.com/CN/Y2016/V47/I10/1954

参考文献

［1］WU B.New discoveries in embryology［M］.Licensee in Tech，2015.http：//dx.doi.org/10.5772/61299.
［2］WILMUT I，SCHNIEKE A E，MCWHIR J，et al.Viable offspring derived from fetal and adult mammalian cells［J］.Nature，1997，385(6619)：810-813.
［3］YIN H，XUE W，CHEN S，et al.Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype［J］.Nat Biotechnol，2014，32：551-553.
［4］LIU X，WANG Y，GUO W，et al.Zinc-finger nickase-mediated insertion of the lysostaphin gene into the beta-casein locus in cloned cows［J］.Nat Commun，2013，4：2565.
［5］WU H，WANG Y，ZHANG Y，et al.TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis［J］.Proc Natl Acad Sci U S A，2014，112(13)：1530-1539.
［6］YU Y，WANG Y，TONG Q，et al.A site-specific recombinase-based method to produce antibiotic selectable marker free transgenic cattle［J］.PLoS One，2013，8(5)：62457.
［7］LAI L，KANG J X，LI B，et al.Generation of cloned transgenic pigs rich in omega-3 fatty acids［J］. Nat Biotechnol，2006，24：435-436.
［8］BETTERIDGE K J.Farm animal embryo technologies：Achievements and perspectives［J］.Theriogenology，2006，65：905-913.
［9］GREGER M.Transgenesis in animal agriculture：addressing animal health and welfare concerns［J］.Agric Environ Ethics，2011，24：451-472.
［10］WU B，ZAN L.Enhance beef cattle improvement by embryo biotechnologies［J］.Reprod Dom Anim，2012，47：865-871.
［11］MAPLETOFT R J.Perspectives on bovine embryo transfer［J］.WCDS Adv Dairy Technol，2012，24：83-93.
［12］HAYES B J，LEWIN H A，GODDARD M E.The future of livestock breeding：genomic selection for efficiency，reduced emissions intensity，and adaptation［J］.Trends Genet，2013，29(4)：206-214.
［13］IBANEZ-ESCRICHE N，FORNI S，NOGUERA J L，et al.Genomic information in pig breeding：Science meets industry needs［J］.Livest Sci，2014(166)：94-100.
［14］HOSSEIN-ZADEH N G.Evaluation of the genetic trend of milk yield in the multiple ovulation and embryo transfer populations of dairy cows，using stochastic simulation［J］.C R Biol，2010(333)：710-715.
［15］PRESICC G A，XU J，GONG G C，et al.Oocyte source and hormonal stimulation for in vitro fertilization using sexed spermatozoa in cattle［J］.Vet Med Int，2011：145626.
［16］DAIRY S J，MEUWISSEN T H.Optimizing pure line breeding strategies utilizing reproductive technologies［J］. J Dairy Sci，1998，81(2)：47-54.
［17］LUO W，WANG Y，ZHANG Y.Simulation study on the efficiencies of MOET nucleus breeding schemes applying marker assisted selection in dairy cattle［J］. Sci China Ser C，2009，52(3)：296-306.
［18］STRANDEN I，MAKI T A，MANTYSAARI E A.Genetic progress and rate of inbreeding in a closed adult MOET nucleus under different mating strategies and heritabilities［J］.J Dairy Sci，1991，108(6)：401-411.
［19］HORTON B.Optimal structure for a three-tiered co-operative sheep breeding system using AI and MOET［J］. Proc Aut Assn Anim Breed Genet，1992，10：435-439.
［20］WILSON D E，MORRICAL D G.The national sheep improvement program：a review［J］.J Anim Sci，1991，69(9)：3872-3881.
［21］NORTHCUTT S L，WILLHAM R L，WILSON D E.Genetic parameters for nuclear and nonnuclear inheritance in three synthetic lines of beef cattle differing in mature size［J］.J Anim Sci，1991，69(12)：4745-4753.
［22］TIAN X C，KUBOTA C，SAKASHITA K，et al.Meat and milk composition of bovine clones［J］.Proc Natl Acad Sci U S A， 2005，102：6261-6266.
［23］YANG X，TIAN X C，KUBOTA C，et al.Risk assessment of meat and milk from cloned animals［J］.Nat Biotechnol，2007，25(1)：77-83.
［24］MAPLETOFT R J，HASLER J F.Assisted reproductive technologies in cattle：a review［J］.Rev Sci Tech，2005，24(1)：393-403.
［25］HANS D D，AURÉLIEN C，HUBERT P，et al.Whole-genome sequencing of 234 bulls facilitates mapping of monogenic and complex traits in cattle［J］.Nat Genet，2014，46(8)：858-865.
［26］DAIRY S J，MEUWISSEN T H.Optimizing pure line breeding strategies utilizing reproductive technologies［J］. J Dairy Sci，1998，81(2)：47-54.
［27］LUO W，WANG Y，ZHANG Y.Simulation study on the efficiencies of MOET nucleus breeding schemes applying marker assisted selection in dairy cattle［J］. Sci China C Life Sci，2009，52(3)：296-306.
［28］BAUER J，PRˇIBYL J，VOSTRY L.Short communication：Reliability of single-step genomic BLUP breeding values by multi-trait test-day model analysis［J］.J Dairy Sci，2015，98(7)：4999-5003.
［29］SU G，MADSEN P，NIELSEN U S，et al.Genomic prediction for Nordic Red cattle using one-step and selection index blending［J］.J Dairy Sci，2012，95(2)：909-917.
［30］HORTON B J，BANKS R G.Industry benefits from using genomic information in two-and three-tier sheep breeding systems［J］.Anim Prod Sci，2015，55(4)：437-446.
［31］张立.牛羊胚胎工程技术产业化研究与应用［D］.杨凌：西北农林科技大学，2005.
ZHANG L.Research and application for industrialization of embryo engineering technology in cattle and sheep［D］.Yangling：Northwest A&F University，2005.(in Chinese)
［32］刘俊平.胚胎工程技术在奶牛繁育中应用的研究［D］.杨凌：西北农林科技大学，2005.
LIU J P.Study on application of embryo engineering technology for reproduction of dairy cows［D］.Yangling：Northwest A&F University，2005.(in Chinese)
［33］权富生.山羊和绵羊超数排卵及规模化胚胎移植技术研究［D］.杨凌：西北农林科技大学，2010.
QUAN F S.Superovulation and large-scale transfer embryo in goats and sheep［D］.Yangling：Northwest A&F University，2010.(in Chinese)
［34］PARAMIO M T.In vivo and in vitro embryo production in goats［J］.Small Ruminant Res，2010，89：144-148.
［35］ZHANG Z P，ZHANG Y，LIU J P，et al.Codeposition of dNTPs detection for rapid LAMP-based sexing of bovine embryos［J］.Reprod Domest Anim，2009，44：116-121.
［36］VLECK L D.Implications of cloning for breed improvement strategies：are traditional methods of animal improvement obsolete?［J］. J Anim Sci，1999，2(1)：111-121.
［37］QUAN F S，ZHANG Z P，AN Z X，et al.Multiple factors affecting superovulation in poll dorset in China［J］.Reprod Domest Anim，2011，46：39-44.
［38］QUAN F S，ZHANG Z P，AN Z X，et al.Effect of transporting donor or recipient does and their embryos on the outcome of fresh embryo transfer in Boer goats［J］.Small Ruminant Res，2010，88：1-5.
［39］GUO J T，AN Z X，YU L，et al.Cloned goats (Capra hircus) from adult ear cells［J］.Sci China Ser C，2002，45：260-267.
［40］WEDEKIND D，WEISS H，JÖRNS A，et al.Effects of polyinosinic-polycytidylic acid and adoptive transfer of immune cells in the Lew.1AR1-iddm rat and in its coisogenic Lew.1AR1 background strain［J］.Autoimmunity，2005，38(4)：265-275.
［41］LIU X，WANG Y，TIAN Y，et al.Generation of mastitis resistance in cows by targeting human lysozyme gene to β-casein locus using zinc-finger nucleases［J］. Proc R Soc B，2014，281：20133368.
［42］WANG H，YANG H，SHIVALILA C S，et al.One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering［J］. Cell，2013，153(4)：910-918.
［43］ZHOU J，SHEN B，ZHANG W，et al.One-step generation of different immunodeficient mice with multiple gene modifications by CRISPR/Cas9 mediated genome engineering［J］.Int J Biochem Cell B，2014，46：49-55.
［44］YANG H，WANG H，SHIVALILA C S，et al.One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering［J］.Cell，2013，154：1370-1379.
［45］SONG J，HUA S，SONG K，et al.Culture，characteristics and chromosome complement of Siberian tiger fibroblasts for nuclear transfer［J］.In vitro Cell Dev Biol Anim，2007，43：203-209.
［46］WELLS D N，MISICA P M，TERVIT H R.Adult somatic cell nuclear transfer is used to preserve the last surviving cow of the Enderly Island cattle breed［J］.Reprod Fert Dev，1998，3：69-78.
［47］LANZA R P，CIBELLI J B，DIAZ F.Cloning of an Endangered species (Bos gaurus) using interspecies nuclear transfer cloning［J］.Cloning，2000(2)：79-90.
［48］PAIVA S R，FACÓ O，FARIA D A，et al.Molecular and pedigree analysis applied to conservation of animal genetic resources：the case of Brazilian Somali hair sheep［J］.Trop Anim Health Pro，2011，43(7)：1449-1457.
［49］MARAA L，CASUB S A，CARTAC M D.Cryobanking of farm animal gametes and embryos as a means of conserving livestock genetics［J］. Anim Reprod Sci，2013，138：25-38.
［50］KARST S，STRUCKEN E M，SCHMITT A O，et al.Effect of the myostatin locus on muscle mass and intramuscular fat content in a cross between mouse lines selected for hypermuscularity［J］.BMC Genom，2013，14：1-16.
［51］LONG Y，RUAN G R，SU Y，et al.Genome-wide association study identifies QTLs for EBV of backfat thickness and average daily gain in Duroc pigs［J］. Russ J Genet，2014，50：1308-1315.
［52］DONG Q，LIU H Y，LI X Y，et al.A genome-wide association study of five meat quality traits in Yorkshire pigs［J］.Front Agric Sci，2014，1(2)：137-143.

[1]	肖艺梅, 王胜男, 许悦雯, 何晓琳, 尹福泉. 热应激对雄性哺乳动物生殖机能影响的研究[J]. 畜牧兽医学报, 2024, 55(1): 11-21.
[2]	陈思颍, 孙雅雯, 李伉, 刘硕, 郝海生, 杜卫华, 邹惠影, 朱化彬, 庞云渭. 微流体技术在家畜体外胚胎生产中的应用进展[J]. 畜牧兽医学报, 2023, 54(12): 4889-4897.
[3]	张宸艺博, 余彤, 任斌斌, 郑睿智, 朱文治, 苏建民. 动物早期胚胎发育中表观重编程的机制[J]. 畜牧兽医学报, 2023, 54(12): 4898-4909.
[4]	杜海东, 娜仁花. 反刍动物妊娠期和泌乳期生理代谢和微生物变化及其对子代发育的影响研究进展[J]. 畜牧兽医学报, 2023, 54(11): 4458-4467.
[5]	邵静, 张颖, 唐毓, 许保增. ERM蛋白在哺乳动物卵母细胞成熟和受精中的研究进展[J]. 畜牧兽医学报, 2023, 54(11): 4477-4487.
[6]	蔡佳炜, 张琛, 靳荣帅, 鲍志远, 张希宇, 王璠, 翟频, 赵博昊, 陈阳, 汤先伟, 吴信生. 热应激下公兔睾丸组织形态和精液转录组分析[J]. 畜牧兽医学报, 2023, 54(11): 4653-4663.
[7]	罗睿杰, 曹素英. 大家畜多能干细胞的研究进展与应用前景[J]. 畜牧兽医学报, 2023, 54(10): 4003-4015.
[8]	邢文文, 齐南南, 李梦轩, 刘吉英. YY1作用机制及在动物繁殖调控中的研究进展[J]. 畜牧兽医学报, 2023, 54(10): 4040-4049.
[9]	何琪富, 高峰, 吴盛辉, 张涌, 权富生. 参与调节哺乳动物精子运动的离子通道研究进展[J]. 畜牧兽医学报, 2023, 54(7): 2708-2722.
[10]	李雯乔, 李发弟, 王新基, 乐祥鹏. 家畜早期妊娠诊断的研究进展[J]. 畜牧兽医学报, 2023, 54(5): 1782-1791.
[11]	秦雪, 沙懿文, 杨梦豪, 蔡瑞, 庞卫军. 非编码RNA调控哺乳动物子宫内膜容受性和蜕膜化的研究进展[J]. 畜牧兽医学报, 2023, 54(4): 1347-1358.
[12]	张培培, 郝海生, 杜卫华, 朱化彬, 李树静, 余文莉, 赵学明. OPU卵母细胞体外成熟体系的优化研究进展[J]. 畜牧兽医学报, 2023, 54(4): 1359-1369.
[13]	刘源壹, 李昕俞, 巴音那木拉, 翠芳, 芒来, 杜明. 单细胞转录组测序技术及其在动物繁殖中的应用进展[J]. 畜牧兽医学报, 2023, 54(2): 421-433.
[14]	段晨莹, 李欣, 赵羚均, 许师源, 原开敏, 董智豪, 郭冠华, 王栋. ART3抑制剂3-MBA对小鼠睾丸生精功能的影响[J]. 畜牧兽医学报, 2022, 53(10): 3470-3479.
[15]	高峰, 何琪富, 吴盛辉, 王少文, 徐雪瑞, 康健, 张涌, 权富生. 哺乳动物配子冷冻保存并应用于珍稀濒危动物保护的技术策略[J]. 畜牧兽医学报, 2022, 53(8): 2479-2489.