基于转录组测序分析METTL14对绵羊骨骼肌卫星细胞成肌分化的影响

doi:10.11843/j.issn.0366-6964.2025.10.014

摘要/Abstract

摘要：

旨在探究甲基转移酶样14(methyltransferase-like 14，METTL14)影响绵羊骨骼肌卫星细胞(skeletal muscle satellite cells，SMSCs)成肌分化的分子机制，为解析METTL14调控绵羊骨骼肌卫星细胞分化的分子机制提供理论基础。本研究对SMSCs进行分离并鉴定，将METTL14过表达质粒及对照质粒分别转染进入SMSCs，通过RT-qPCR和Western blot检测其过表达效率及METTL14影响SMSCs分化的功能。对SMSCs过表达METTL14组(OE-14)和对照组(OE-NC)进行转录组测序，以P＜0.05且log₂|Fold Change|＞1为阈值鉴定差异表达基因，进行GO和KEGG富集分析及PPI蛋白质网络互作分析。METTL14过表达能够显著增加SMSCs中METTL14的表达及成肌分化相关基因(MyHC、MyoG)的mRNA和蛋白水平，表明METTL14过表达成功，且METTL14能够促进SMSCs的分化。两组中共鉴定到259个差异表达基因，与OE-NC相比，OE-14共有45个基因表达上调，214个基因表达下调。差异基因主要富集到细胞核通路、Rap1信号通路等。PPI蛋白质网络互作分析筛选出8个枢纽基因(Degree≥15), 包括MX1、RSAD2、IFIH1、DDX58、HERC5、ISG15、MX2以及IRF7。本研究表明METTL14通过影响细胞进程和肌肉发育相关的基因和信号通路促进绵羊骨骼肌卫星细胞的分化。

关键词: METTL14, 转录组测序, 骨骼肌卫星细胞, 绵羊, 细胞分化

Abstract:

The aim of this study was to explore the molecular mechanism underlying the effect of methyltransferase like 14 (METTL14) on the myogenic differentiation of sheep skeletal muscle satellite cells(SMSCs) and to provide a theoretical basis for analyzing the molecular mechanism of METTL14 regulating the differentiation of SMSCs. SMSCs were isolated and identified. The overexpression plasmid of METTL14 and the control plasmid were transfected into SMSCs, and the overexpression efficiency and the function of METTL14 affecting SMSCs differentiation were detected by RT-qPCR and Western blot. RNA-seq was performed for METTL14-overexpressed SMSCs(OE-14) and control SMSCs(OE-NC). Differentially expressed genes (DEGs) were identified under the conditions of P < 0.05 and log₂|Fold Change| > 1, GO and KEGG enrichment analysis and protein-protein interaction (PPI) network interaction analysis for DEGs was conducted. Overexpression of METTL14 markedly elevated the expression of METTL14, as well as the mRNA and protein levels of myogenic differentiation-related genes (MyHC, MyoG) in ovine SMSCs, thereby demonstrating successful METTL14 overexpression and the enhancement of SMSCs differentiation mediated by METTL14. A total of 259 differentially expressed genes were identified between the two groups. Compared with the OE-NC group, the OE-14 group had 45 up-regulated genes and 214 down-regulated genes. Differential genes were mainly enriched in nuclear pathways, Rap1 signaling pathways, etc. PPI protein network interaction analysis identified 8 hub genes (Degree≥15), including MX1, RSAD2, IFIH1, DDX58, HERC5, ISG15, MX2, and IRF7. This study indicated that METTL14 can promote the differentiation of ovine SMSCs through influencing genes and signaling pathways related to cellular processes and muscle development.

Key words: METTL14, transcriptome sequencing, skeletal muscle satellite cells, sheep, cell differentiation

中图分类号:

S826.2

何思琦, 陈倩, 蒋琳, 马月辉, 周胜花, 赵倩君. 基于转录组测序分析METTL14对绵羊骨骼肌卫星细胞成肌分化的影响[J]. 畜牧兽医学报, 2025, 56(10): 4925-4937.

HE Siqi, CHEN Qian, JIANG Lin, MA Yuehui, ZHOU Shenghua, ZHAO Qianjun. The Effect of METTL14 on Myogenic Differentiation of Ovine Skeletal Muscle Satellite Cells Based on Transcriptome Sequencing Analysis[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(10): 4925-4937.

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参考文献 56

1	李雪娇, 刘晨曦, 孙亚伟, 等. 德国美利奴羊胎儿期骨骼肌组织学结构发育特征研究[J]. 西北农林科技大学学报(自然科学版), 2018, 46 (5): 1- 7.
	LI X J , LIU C X , SUN Y W , et al. Study on structure development characteristics of German Merino sheep fetal skeletal muscle tissue[J]. Journal of Northwest A & F University(Natural Science Edition), 2018, 46 (5): 1- 7.
2	李伯江, 李平华, 吴望军, 等. 骨骼肌肌纤维形成机制的研究进展[J]. 中国农业科学, 2014, 47 (6): 1200- 1207.
	LI B J , LI P H , WU W J , et al. Progresses in research of the mechanisms of skeletal muscle fiber Formation[J]. Scientia Agricultura Sinica, 2014, 47 (6): 1200- 1207.
3	BISCHOFF R . Enzymatic liberation of myogenic cells from adult rat muscle[J]. Anat Rec, 1974, 180 (4): 645- 661. doi: 10.1002/ar.1091800410
4	DODSON M V , MARTIN E L , BRANNON M A , et al. Optimization of bovine satellite cell-derived myotube formation in vitro[J]. Tissue Cell, 1987, 19 (2): 159- 166. doi: 10.1016/0040-8166(87)90001-2
5	WU H , REN Y , LI S , et al. In vitro culture and induced differentiation of sheep skeletal muscle satellite cells[J]. Cell Biol Int, 2012, 36 (6): 579- 587. doi: 10.1042/CBI20110487
6	LIU H H , LI L , CHEN X , et al. Characterization of in vitro cultured myoblasts isolated from duck (Anas platyrhynchos) embryo[J]. Cytotechnology, 2011, 63 (4): 399- 406. doi: 10.1007/s10616-011-9356-7
7	SEALE P , SABOURIN L A , GIRGIS-GABARDO A , et al. Pax7 is required for the specification of myogenic satellite cells[J]. Cell, 2000, 102 (6): 777- 786. doi: 10.1016/S0092-8674(00)00066-0
8	SCHULTZ E . Satellite cell behavior during skeletal muscle growth and regeneration[J]. Med Sci Sports Exerc, 1989, 21 (5 Suppl): S181- 186.
9	WHITE T P , ESSER K A . Satellite cell and growth factor involvement in skeletal muscle growth[J]. Med Sci Sports Exerc, 1989, 21 (5 Suppl): S158- 163.
10	WAGERS A J , CONBOY I M . Cellular and molecular signatures of muscle regeneration: current concepts and controversies in adult myogenesis[J]. Cell, 2005, 122 (5): 659- 667. doi: 10.1016/j.cell.2005.08.021
11	VON MALTZAHN J , JONES A E , PARKS R J , et al. Pax7 is critical for the normal function of satellite cells in adult skeletal muscle[J]. Proc Natl Acad Sci U S A, 2013, 110 (41): 16474- 16479. doi: 10.1073/pnas.1307680110
12	FENG X , NAZ F , JUAN A H , et al. Identification of skeletal muscle satellite cells by immuno-fluorescence with Pax7 and laminin antibodies[J]. J Vis Exp, 2018 (134): 57212.
13	李欣, 于永生, 张立春, 等. 绵羊骨骼肌卫星细胞分离培养、鉴定与成肌诱导分化[J]. 中国畜牧兽医, 2021, 48 (4): 1204- 1210.
	LI X , YU Y S , ZHANG L C , et al. Isolation, culture, identification and myognic differentiation of sheep skeletal muscle satellite cells[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48 (4): 1204- 1210.
14	DHAWAN J , RANDO T A . Stem cells in postnatal myogenesis: molecular mechanisms of satellite cell quiescence, activation and replenishment[J]. Trends Cell Biol, 2005, 15 (12): 666- 673. doi: 10.1016/j.tcb.2005.10.007
15	CORNELISON D D , WOLD B J . Single-cell analysis of regulatory gene expression in quiescent and activated mouse skeletal muscle satellite cells[J]. Dev Biol, 1997, 191 (2): 270- 283. doi: 10.1006/dbio.1997.8721
16	张笑, 贾桂芳. RNA表观遗传修饰: N6-甲基腺嘌呤[J]. 遗传, 2016, 38 (4): 275- 288.
	ZHANG X , JIA G F . RNA epigenetic modification: N6-methyladenosine[J]. Hereditas, 2016, 38 (4): 275- 288.
17	LIU J , YUE Y , HAN D , et al. A METTL3-METTL14 complex mediates mammalian nuclear RNA N6-adenosine methylation[J]. Nat Chem Biol, 2014, 10 (2): 93- 95. doi: 10.1038/nchembio.1432
18	WANG P , DOXTADER K A , NAM Y . Structural basis for cooperative function of Mettl3 and Mettl14 methyltransferases[J]. Mol Cell, 2016, 63 (2): 306- 317. doi: 10.1016/j.molcel.2016.05.041
19	YANG X , MEI C , MA X , et al. m(6)A methylases regulate myoblast proliferation, apoptosis and differentiation[J]. Animals (Basel), 2022, 12 (6): 773.
20	CHEN B , LIU S , ZHANG W , et al. Profiling analysis of N6-methyladenosine mRNA methylation reveals differential m6A patterns during the embryonic skeletal muscle development of ducks[J]. Animals (Basel), 2022, 12 (19): 2593.
21	JIANG Q , XU T , ZHOU H , et al. METTL14 regulates proliferation and differentiation of duck myoblasts through targeting MiR-133b[J]. PLoS One, 2025, 20 (3): e0320659. doi: 10.1371/journal.pone.0320659
22	PETROSINO J M , HINGER S A , GOLUBEVA V A , et al. The m(6)A methyltransferase METTL3 regulates muscle maintenance and growth in mice[J]. Nat Commun, 2022, 13 (1): 168. doi: 10.1038/s41467-021-27848-7
23	TAN B , ZENG J , MENG F , et al. Comprehensive analysis of pre-mRNA alternative splicing regulated by m6A methylation in pig oxidative and glycolytic skeletal muscles[J]. BMC Genomics, 2022, 23 (1): 804. doi: 10.1186/s12864-022-09043-0
24	束婧婷, 单艳菊, 姬改革, 等. 广西麻鸡m6A甲基转移酶基因表达与肌纤维类型及成肌分化的关系[J]. 中国农业科学, 2022, 55 (3): 589- 601.
	SHU J T , SHAN Y J , JI G G , et al. Relationship between expression levels of guangxi partridge chicken m6A methyltransferase genes, myofiber types and myogenic differentiation[J]. Scientia Agricultura Sinica, 2022, 55 (3): 589- 601.
25	马兰花. METTL14基因在牦牛前体脂肪细胞增殖分化和脂质沉积中的作用[D]. 兰州: 西北民族大学, 2022.
	MA L H. The role of METTL14 gene in proliferation, differentiationand lipid deposition of yak preadipocytes[D]. Lanzhou: Northwest Minzu University, 2022. (in Chinese)
26	张丹, 陈博雯, 杨博辉, 等. 湖羊m6A甲基转移酶METTL14基因CDS序列克隆及表达谱分析[J]. 南方农业学报, 2023, 54 (10): 3047- 3055.
	ZHANG D , CHEN B W , YANG B H , et al. Cloning and expression profile analysis of CDS sequence of m6A methyltransferase METTL14 gene from Hu sheep[J]. Journal of Southern Agriculture, 2023, 54 (10): 3047- 3055.
27	丁浩, 林月月, 张涛, 等. m6A甲基化在鸡肌肉生长发育中的表达研究[J]. 中国畜牧兽医, 2021, 48 (5): 1525- 1534.
	DING H , LIN Y Y , ZHANG T , et al. Study on the Expression of m6A Methylation in Chicken Muscle Growth and Development[J]. China Animal Husbandry & Veterinary Medicine, 2021, 48 (5): 1525- 1534.
28	杨海平, 戴敏, 张东华. 细胞因子信号传导抑制蛋白-1(SOCS-1)研究进展[J]. 中国实验血液学杂志, 2007 (2): 437- 440.
	YANG H P , DAI M , ZHANG D H . Progress of study on suppressor of cytokine signaling-1——review[J]. Journal of Experimental Hematology, 2007 (2): 437- 440.
29	DEYHLE M R , HAFEN P S , PARMLEY J , et al. CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration[J]. Physiol Rep, 2018, 6 (8): e13689. doi: 10.14814/phy2.13689
30	BERKES C A , TAPSCOTT S J . MyoD and the transcriptional control of myogenesis[J]. Semin Cell Dev Biol, 2005, 16 (4-5): 585- 595. doi: 10.1016/j.semcdb.2005.07.006
31	MASCARELLO F , TONIOLO L , CANCELLARA P , et al. Expression and identification of 10 sarcomeric MyHC isoforms in human skeletal muscles of different embryological origin. Diversity and similarity in mammalian species[J]. Ann Anat, 2016, 207, 9- 20. doi: 10.1016/j.aanat.2016.02.007
32	XIE S J , LEI H , YANG B , et al. Dynamic m(6)A mRNA methylation reveals the role of METTL3/14-m(6)A-MNK2-ERK signaling axis in skeletal muscle differentiation and regeneration[J]. Front Cell Dev Biol, 2021, 9, 744171. doi: 10.3389/fcell.2021.744171
33	董可为, 沈尧, 王帅, 等. WTAP通过上调糖酵解抑制结直肠癌细胞分化[J]. 空军军医大学学报, 2024, 45 (3): 298-302+310.
	DONG K W , SHEN Y , WANG S , et al. WTAP inhibits colorectal cancer cell differentiation by up-regulating glycolysis[J]. Journal of Air Force Medical University, 2024, 45 (3): 298-302+310.
34	刘铃, 王圣楠, 王丹丹, 等. Zbed6基因敲除对小鼠骨骼肌生长发育的影响及其分子作用机制研究[J]. 中国畜牧兽医, 2023, 50 (9): 3641- 3651.
	LIU L , WANG S N , WANG D D , et al. Effect and molecular mechanism of Zbed6 gene knockout on the frowth and development of skeletal muscle in mice[J]. China Animal Husbandry & Veterinary Medicine, 2023, 50 (9): 3641- 3651.
35	GAO X Q , ZHANG Y H , LIU F , et al. The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N(6)-methyladenosine methylation of Parp10 mRNA[J]. Nat Cell Biol, 2020, 22 (11): 1319- 1331. doi: 10.1038/s41556-020-0576-y
36	LIU Z , ZHANG X , LEI H , et al. CASZ1 induces skeletal muscle and rhabdomyosarcoma differentiation through a feed-forward loop with MYOD and MYOG[J]. Nat Commun, 2020, 11 (1): 911. doi: 10.1038/s41467-020-14684-4
37	AMIN N M , GIBBS D , CONLON F L . Differential regulation of CASZ1 protein expression during cardiac and skeletal muscle development[J]. Dev Dyn, 2014, 243 (7): 948- 956. doi: 10.1002/dvdy.24126
38	KAMALUDIN A A , SMOLARCHUK C , BISCHOF J M , et al. Muscle dysfunction caused by loss of Magel2 in a mouse model of Prader-Willi and Schaaf-Yang syndromes[J]. Hum Mol Genet, 2016, 25 (17): 3798- 3809. doi: 10.1093/hmg/ddw225
39	FERREIRA F J , CARVALHO L , LOGARINHO E , et al. foxm1 modulates cell non-autonomous response in zebrafish skeletal muscle homeostasis[J]. Cells, 2021, 10 (5): 1241. doi: 10.3390/cells10051241
40	NEJAD F M , MOHAMMADABADI M , ROUDBARI Z , et al. Network visualization of genes involved in skeletal muscle myogenesis in livestock animals[J]. BMC Genomics, 2024, 25 (1): 294. doi: 10.1186/s12864-024-10196-3
41	KITAJIMA Y , YOSHIOKA K , SUZUKI N . The ubiquitin-proteasome system in regulation of the skeletal muscle homeostasis and atrophy: from basic science to disorders[J]. J Physiol Sci, 2020, 70 (1): 40. doi: 10.1186/s12576-020-00768-9
42	PIZON V , MÉCHALI F , BALDACCI G . RAP1A GTP/GDP cycles determine the intracellular location of the late endocytic compartments and contribute to myogenic differentiation[J]. Exp Cell Res, 1999, 246 (1): 56- 68. doi: 10.1006/excr.1998.4284
43	HAO D , WANG X , WANG X , et al. Transcriptomic changes in bovine skeletal muscle cells after resveratrol treatment[J]. Gene, 2020, 754, 144849. doi: 10.1016/j.gene.2020.144849
44	WANG Y , WANG J , HU H , et al. Dynamic transcriptome profiles of postnatal porcine skeletal muscle growth and development[J]. BMC Genom Data, 2021, 22 (1): 32. doi: 10.1186/s12863-021-00984-1
45	ZHAN S , ZHAO W , SONG T , et al. Dynamic transcriptomic analysis in hircine longissimus dorsi muscle from fetal to neonatal development stages[J]. Funct Integr Genomics, 2018, 18 (1): 43- 54. doi: 10.1007/s10142-017-0573-9
46	MOHAMMADINEJAD F , MOHAMMADABADI M , ROUDBARI Z , et al. Identification of key genes and biological pathways associated with skeletal muscle maturation and hypertrophy in Bos taurus, Ovis aries, and Sus scrofa[J]. Animals (Basel), 2022, 12 (24): 3471.
47	GLICKMAN M H , CIECHANOVER A . The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction[J]. Physiol Rev, 2002, 82 (2): 373- 428. doi: 10.1152/physrev.00027.2001
48	OLGUÍN H C . The gentle side of the UPS: Ubiquitin-proteasome system and the regulation of the myogenic program[J]. Front Cell Dev Biol, 2021, 9, 821839.
49	HORISBERGER M A . Interferon-induced human protein MxA is a GTPase which binds transiently to cellular proteins[J]. J Virol, 1992, 66 (8): 4705- 4709. doi: 10.1128/jvi.66.8.4705-4709.1992
50	ELLINWOOD N M , MCCUE J M , GORDY P W , et al. Cloning and characterization of cDNAs for a bovine (Bos taurus) Mx protein[J]. J Interferon Cytokine Res, 1998, 18 (9): 745- 755. doi: 10.1089/jir.1998.18.745
51	SCARAMOZZA A , PARK D , KOLLU S , et al. Lineage tracing reveals a subset of reserve muscle stem cells capable of Clonal expansion under stress[J]. Cell Stem Cell, 2019, 24 (6): 944- 957. doi: 10.1016/j.stem.2019.03.020
52	CHISTIAKOV D A . Interferon induced with helicase C domain 1 (IFIH1) and virus-induced autoimmunity: a review[J]. Viral Immunol, 2010, 23 (1): 3- 15. doi: 10.1089/vim.2009.0071
53	WANG Q , XU J , BAO M , et al. Weighted gene co-expression network analysis reveals genes related to growth performance in Hu sheep[J]. Sci Rep, 2024, 14 (1): 13043. doi: 10.1038/s41598-024-63850-x
54	MORESI V , ADAMO S , BERGHELLA L . The JAK/STAT pathway in skeletal muscle pathophysiology[J]. Front Physiol, 2019, 10, 500. doi: 10.3389/fphys.2019.00500
55	TRENERRY M K , DELLA GATTA P A , CAMERON-SMITH D . JAK/STAT signaling and human in vitromyogenesis[J]. BMC Physiol, 2011, 11, 6. doi: 10.1186/1472-6793-11-6
56	HUANG L , ZHANG S M , ZHANG P , et al. Interferon regulatory factor 7 protects against vascular smooth muscle cell proliferation and neointima formation[J]. J Am Heart Assoc, 2014, 3 (5): e001309. doi: 10.1161/JAHA.114.001309

基因 Gene	引物序列(5′→3′) Primer sequence	登录号 Accession number	退火温度/℃ Annealing temperature	产物大小/bp Product size
METTL14	F：AGATTGCAGCACCTCGATCA	XM_004009592.6	60	87
	R：CCCACTTGCGTAAACACACT
MyHC	F：CTGTCCAAGTTCCGCAAGGT	XM_004010325.4	60	182
	R：GAGCTTCGTTGCACCCTCAA
MyoG	F：GAGAACTACCTGCCTGTCCAC	NM_001174109.1	60	251
	R：GCCTCGAAGGCTTCATTCAC
MX2	F：ACTGGGGCAGACAATGAGTC	NM_001078652.1	60	149
	R：GGTTGTTTTCGGACCCCTTT
RSAD2	F：TGGTTCCAGAAGTACGGTGA	XM_004005669.5	60	79
	R：TAAGGACGTTGACCTGCTCG
IFI35	F：GCGGGGACTTCAACGAAAGG	XM_004012955.6	60	182
	R：GCCTGGAGAGCCGACACAG
PGAM2	F：GTCCATCAGCAAGGAGCGT	XM_004018189.5	60	186
	R：TGCTTGACGATTCCCCGTAG
RERG	F：CAACCTACCGACACCAAGCA	XM_012175305.4	60	86
	R：CTGAATGGTGTCTTCCTGCCC
GAPDH	F：GGGTCATCATCTCTGCACCT	NM_001190390.1	60	176
	R：GGTCATAAGTCCCTCCACGA

样本 Sample	原始碱基数/bp Raw datas	过滤后碱基数/bp Clean datas	过滤后读数 Clean reads	比对率/% Ratio	Q20/%	Q30/%	GC含量/% GC content
OE-NC-1	11 617 995 000	11 478 734 663	77 185 284	95.59	98.02	94.12	48.98
OE-NC-2	11 786 511 900	11 660 724 465	78 254 396	95.51	97.97	94.01	48.81
OE-NC-3	11 790 966 300	11 668 905 187	78 291 154	95.73	97.97	94.02	49.21
OE-14-1	12 070 909 800	11 943 452 847	80 182 260	95.55	98.03	94.14	49.85
OE-14-2	11 967 072 600	11 842 913 088	79 510 606	95.52	98.03	94.12	49.12
OE-14-3	12 023 415 300	11 914 845 248	79 901 354	95.59	97.99	93.94	49.61

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