硒多糖缓解马骨骼肌卫星细胞氧化损伤作用的研究

doi:10.11843/j.issn.0366-6964.2025.07.028

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (7): 3357-3367.doi: 10.11843/j.issn.0366-6964.2025.07.028

硒多糖缓解马骨骼肌卫星细胞氧化损伤作用的研究

刘雨蒙(), 高星, 赵雅丽, 曹迪, 芒来, 张心壮*()

内蒙古农业大学动物科学学院农业农村部马属动物种业创新重点实验室, 呼和浩特 010018
内蒙古农业大学动物科学学院内蒙古马属动物科学研究与技术创新重点实验室, 呼和浩特 010018
内蒙古农业大学动物科学学院内蒙古农业大学马属动物研究中心, 呼和浩特 010018

收稿日期:2024-08-29 出版日期:2025-07-23 发布日期:2025-07-25
通讯作者: 张心壮 E-mail:1622969534@qq.com;ZhangXinZhuang@126.com
作者简介:刘雨蒙(1999-)，女，黑龙江大庆人，硕士，主要从事马饲料营养研究，E-mail: 1622969534@qq.com
基金资助:
内蒙古农业大学优秀青年科学基金培育项目(BR230405)；内蒙古自治区重点研发项目(2023YFD0002)；内蒙古自治区教育厅一流学科科研专项(YLXKZX-NND-007)；内蒙古农业大学动物科学学院高水平成果培育专项(CG202421)

Effects of Selenium Polysaccharides on Oxidative Damage of Equine Skeletal Muscle Satellite Cell

LIU Yumeng(), GAO Xing, ZHAO Yali, CAO Di, MANG Lai, ZHANG Xinzhuang*()

Key Laboratory of Equine Animal Industry Innovation, Ministry of Agriculture, Inner Mongolia Agricultural University, Hohhot 010018, China
Rural Affairs/Inner Mongolia Key Laboratory of Equine Animal Scientific Research, Inner Mongolia Agricultural University, Hohhot 010018, China
Technological Innovation/Inner Mongolia Agricultural University, Equine Animal Research Center, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2024-08-29 Online:2025-07-23 Published:2025-07-25
Contact: ZHANG Xinzhuang E-mail:1622969534@qq.com;ZhangXinZhuang@126.com

摘要/Abstract

摘要：

旨在研究硒多糖预保护对H₂O₂诱导蒙古马骨骼肌卫星细胞氧化应激的缓解作用及机制，为开发缓解马的骨骼肌运动损伤抗氧化剂提供参考。利用H₂O₂诱导的蒙古马骨骼肌卫星细胞氧化损伤模型开展试验，试验共分为对照组、H₂O₂损伤组以及硒多糖保护组(硒多糖保护组的浓度分别为50、150、250、350、450、550 nmol ·L^-1)，对细胞活力、抗氧化性能、抗氧化基因表达量和细胞线粒体功能进行检测。结果表明：随着硒多糖预保护浓度的增加，细胞存活率、总抗氧化能力(total antioxidant capacity，T-AOC)、超氧化物歧化酶(superoxide dismutase，SOD)、过氧化氢酶(catalase，CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase，GPX)的活性出现先增加后降低的变化，350 nmol ·L^-1硒多糖预保护组细胞存活率显著高于H₂O₂损伤组(P＜0.05)，与对照组无显著差异，但抗氧化酶活性显著高于H₂O₂损伤组和对照组(P＜0.05)；细胞中丙二醛(malondialdehyde，MDA)含量随硒多糖的增加呈先降低后增高的变化，350 nmol ·L^-1硒多糖预保护组含量最低，显著低于对照组和H₂O₂损伤组(P＜0.05)；350 nmol ·L^-1硒多糖预保护组原癌基因RELA(RELA proto-oncogene，RELA)、谷胱甘肽过氧化物酶(probable phospholipid hydroperoxide glutathione peroxidase，GPX)、硫氧还蛋白还原酶1(thioredoxin reductase 1，TRXR1)和超氧化物歧化酶1(superoxide dismutase 1，SOD1)的mRNA表达量极显著高于H₂O₂损伤组(P＜0.01)，RELA的mRNA表达量显著高于对照组(P＜0.05)，GPX、TRXR1和SOD1的mRNA表达量极显著高于对照组(P＜0.01)。350 nmol ·L^-1硒多糖预保护组核因子类红细胞2相关因子2(nuclear factor erythroid-2-related factor 2，Nrf2)的mRNA表达量显著高于其他两组(P＜0.05)；350 nmol ·L^-1硒多糖组的细胞耗氧率(OCR)显著高于H₂O₂损伤组(P < 0.05)，基础呼吸值、ATP消耗值、质子漏、最大呼吸值分别为34.86、26.06、8.80、32.41，极显著高于H₂O₂损伤组(P < 0.01)，非线粒体呼吸值显著高于损伤组(P < 0.05)。综上可知，硒多糖通过激活Nrf2信号通路，上调下游抗氧化基因的表达，进而提高了抗氧化酶活性，降低了MDA的含量，促进了细胞线粒体呼吸功能，提高了细胞活性，达到缓解马骨骼肌卫星细胞氧化损伤的效果，本试验条件下，350 nmol ·L^-1硒多糖效果较好。

关键词: 硒多糖, 马, 骨骼肌卫星细胞, 氧化损伤

Abstract:

The aim of this study was to investigate the protective effect and mechanism of selenium (Se) polysaccharide (Sepol) preprotection on H₂O₂-induced oxidative stress in Mongolian horse skeletal muscle satellite cells, and to provide a reference for the development of antioxidants to alleviate exercise injury in horse skeletal muscle.The oxidative injury model of Mongolian horse skeletal muscle satellite cells was induced by H₂O₂ and used to perform the experiment. The experiment was divided into control group, H₂O₂ injury group and selenium polysaccharide protection groups (concentrations of selenium polysaccharide protection group were 50, 150, 250, 350, 450, 550 nmol ·L^-1, respectively). Cell viability, antioxidant activity, antioxidant gene expression and mitochondrial function were measured.The results showed that: Cell viability, total antioxidant capacity (T-AOC), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) increased first and then decreased with the increase of pre-protective concentration of seleniumpolysaccharide. The cell survival rate of 350 nmol ·L^-1 Se polysaccharide preprotection group was significantly higher than that of injury group (P < 0.05) and there was no significant difference between 350 nmol ·L^-1 Se polysaccharide preprotection group and control group, but the activity of antioxidant enzymes was significantly higher than that of injury group and control group (P < 0.05). The content of malondialdehyde (MDA) in cells decreased first and then increased with the increase of Se polysaccharide. The content of MDA in 350 nmol ·L^-1 Se polysaccharide preprotection group was the lowest, which was significantly lower than that in control group and injury group (P < 0.05).The mRNA expression of proto-oncogene RELA(RELA proto-oncogene, RELA), glutathione peroxidase (probable phospholipid hydroperoxide glutathione peroxidase, GPX), thioredoxin reductase 1 (TRXR 1 ) and superoxide dismutase 1 (SOD 1 ) in the 350 nmol ·L^-1 selenium polysaccharide pre-protected group was highly significant higher than that of the injured group (P < 0.01). The mRNA expression of RELA was significantly higher than that of the control group (P < 0.05), the mRNA expression of GPX, TRXR1 and SOD1 was extremely significantly higher than those of the control group (P < 0.01). mRNA expression of the nuclear factor-like erythroid 2-related factor 2 (Nrf2 ) in the 350 nmol ·L^-1 selenium polysaccharide preprotected group was significantly higher than that of the other two selenium polysaccharide preprotected groups (P < 0.05).The oxygen consumption rate (OCR) of 350 nmol ·L^-1selenosaccharide group was significantly higher than that of H₂O₂injury group (P < 0.05).The basal respiration, ATP consumption, proton leak and maximal respiration were 34.86, 26.06, 8.80 and 32.41, respectively, which were significantly higher than those of H₂O₂ injury group (P < 0.01). The non-mitochondrial respiration value was significantly higher than that of the injury group (P < 0.05).In conclusion, selenium polysaccharide can alleviate oxidative damage of horse skeletal muscle satellite cells by activating Nrf2 signaling pathway and up-regulating the expression of downstream antioxidant genes, thereby improving antioxidant enzyme activity, reducing MDA content, promoting mitochondrial respiratory function, and improving cell activity. Under the current experimental conditions, 350 nmol ·L^-1 selenium polysaccharide had the best effect.

Key words: selenium polysaccharide, horse, skeletal muscle satellite cells, oxidative damage

中图分类号:

S821.5

刘雨蒙, 高星, 赵雅丽, 曹迪, 芒来, 张心壮. 硒多糖缓解马骨骼肌卫星细胞氧化损伤作用的研究[J]. 畜牧兽医学报, 2025, 56(7): 3357-3367.

LIU Yumeng, GAO Xing, ZHAO Yali, CAO Di, MANG Lai, ZHANG Xinzhuang. Effects of Selenium Polysaccharides on Oxidative Damage of Equine Skeletal Muscle Satellite Cell[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3357-3367.

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参考文献 47

1	李志平. 国际马产业发展经验及启示[J]. 世界农业, 2020 (2): 98-104, 114.
	LI Z P . International horse industry development experience and inspiration[J]. World Agriculture, 2020 (2): 98-104, 114.
2	BRKLJAČA BOTTEGARO N , GOTIĆ J , ŠURAN J , et al. Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses[J]. BMC Vet Res, 2018, 14 (1): 216.
3	WILLIAMS C A , KRONFELD D S , HESS T M , et al. Vitamin E intake and systemic antioxidant status in competitive endurance horses[J]. Equine and Comparative Exercise Physiology, 2005, 2 (3): 149- 152.
4	WANG D , CHEN J , SUN H , et al. MCFA alleviate H₂O₂-induced oxidative stress in AML12 cells via the ERK1/2/Nrf2 pathway[J]. Lipids, 2022, 57 (3): 153- 162.
5	WILLIAMS C A , KRONFELDT D S , HESS T M , et al. Antioxidant supplementation and subsequent oxidative stress of horses during an 80-km endurance race[J]. J Anim Sci, 2004, 82 (2): 588- 594.
6	CHAN A C . Partners in defense, vitamin E and vitamin C[J]. Can J Physiol Pharmacol, 1993, 71 (9): 725- 731.
7	WHITE S H , WOHLGEMUTH S , LI C , et al. Rapid Communication: Dietary selenium improves skeletal muscle mitochondrial biogenesis in young equine athletes[J]. J Anim Sci, 2017, 95 (9): 4078- 4084.
8	GARCIA E I C , ELGHANDOUR M M M Y , KHUSRO A , et al. Dietary supplements of vitamins E, C, and β-carotene to reduce oxidative stress in horses: An overview[J]. J Equine Vet Sci, 2022, 110, 103863.
9	李梅林, 杜津昊, 刘建飞, 等. 枸杞硒多糖的合成及对人体肝癌HepG2细胞增殖的体外抑制作用评价[J]. 食品工业科技, 2018, 39 (11): 22- 27.
	LI M L , DU J H , LIU J F , et al. Synthesis of selenium polysaccharide from Lycium barbarum and its inhibitory effect on HepG2 cell growth in vitro[J]. Science and Technology of Food Industry, 2018, 39 (11): 22- 27.
10	景磊, 王婷婷, 胡晨曦, 等. 超声辅助纤维素酶法提取小麦硒多糖工艺优化及抗氧化活性研究[J]. 河南城建学院学报, 2024, 33 (2): 122- 128.
	JING L , WANG T T , HU C X , et al. Optimization of ultrasound-assisted cellulase extraction of selenium polysaccharide from wheat and its antioxidant activity[J]. Journal of Henan University of Urban Construction, 2024, 33 (2): 122- 128.
11	周美, 姜阳明, 国光梅, 等. 铁皮石斛硒多糖制备及免疫活性[J]. 现代食品科技, 2025, 41 (2): 9- 18.
	ZHOU M , JIANG Y M , GUO G M , et al. Preparation and immune activity of selenated dendrobium officinale polysaccharide[J]. Modern Food Science and Technology, 2025, 41 (2): 9- 18.
12	张梦圆, 朱晓庆, 谷新利, 等. 硒化甘草多糖、甘草多糖及其联合抗生素对无乳链球菌体外抗菌活性及机制分析[J]. 新疆农业科学, 2024, 61 (2): 469- 478.
	ZHANG M Y , ZHU X Q , GU X L , et al. Study on antibacterial activity and mechanism of selenium glycyrrhiza polysaccharide, glycyrrhiza polysaccharide and their combined antibiotics against Streptococcus agalactiae in vitro[J]. Xinjiang Agricultural Sciences, 2024, 61 (2): 469- 478.
13	杨雪. 枸杞多糖纳米硒体外消化吸收特性及抗疲劳活性研究[D]. 扬州: 扬州大学, 2021.
	YANG X. Study on simulated digestion and absorption in vitro and anti-fatigue activity of selenium nanoparticles stabilized by Lycium barbarum polysaccharide[D]. Yangzhou: Yangzhou University, 2021. (in Chinese)
14	HAMID M , LIU D , ABDULRAHIM Y , et al. Inactivation of Kupffer cells by selenizing Astragalus polysaccharides prevents CCl₄-induced hepatocellular necrosis in the male Wistar rat[J]. Biol Trace Elem Res, 2017, 179, 226- 236.
15	CHEN D , SUN H , SHEN Y , et al. Selenium bio-absorption and antioxidant capacity in mice treated by selenium modified rice germ polysaccharide[J]. Journal of Functional Foods, 2019, 61, 103492.
16	赵雅丽. Nrf2-ARE信号通路介导的马骨骼肌卫星细胞氧化应激的调控机理[D]. 呼和浩特: 内蒙古农业大学, 2020.
	ZHAO Y L. The regulatory mechanism of oxidative stress in horse skeletalative muscle satellite cels mediated by Nrf2-ARE signaling pathway[D]. Hohhot: Inner Mongolia Agricultural University, 2020. (in Chinese)
17	SCHERZ-SHOUVAL R , ELAZAR Z . Regulation of autophagy by ROS: physiology and pathology[J]. Trends Biochem Sci, 2011, 36 (1): 30- 38.
18	詹经纬, 李欣, 关淑文, 等. 竹叶黄酮对过氧化氢诱导的奶牛乳腺上皮细胞氧化损伤的保护作用[J]. 动物营养学报, 2023, 35 (4): 2616- 2628.
	ZHAN J W , LI X , GUAN S W , et al. Protective effect of bamboo leaf flavonoids on oxidative damage of bovine mammary epithelial cells induced by hydrogen peroxide[J]. Chinese Journal of Animal Nutrition, 2023, 35 (4): 2616- 2628.
19	李永义. 茶多酚对氧化应激仔猪的保护作用及机制研究[D]. 雅安: 四川农业大学, 2011.
	LI Y Y. Protective effects of tea polyphenols for weaned pigs challenged with oxidative stress[D]. Yaan: Sichuan Agricultural University, 2011. (in Chinese)
20	齐晓龙, 赵芹, 张亚男, 等. 过氧化氢诱导产蛋鸡原代肝细胞氧化应激模型的建立[J]. 中国畜牧杂志, 2013, 49 (11): 49- 52.
	QI X L , ZHAO Q , ZHANG Y N , et al. Establishment of a model of hydrogen peroxide-induced oxidative stress in primary hepatocytes in laying hens[J]. Chinese Journal of Animal Science, 2013, 49 (11): 49- 52.
21	SCHRAUZER G N . Anticarcinogenic effects of selenium[J]. Cell Mol Life Sci, 2000, 57 (13-14): 1864- 1873.
22	WANG L , XIAO J X , HUA Y , et al. Effects of dietary selenium polysaccharide on growth performance, oxidative stress and tissue selenium accumulation of juvenile black sea bream, Acanthopagrus schlegelii[J]. Aquaculture, 2019, 503, 389- 395.
23	YANG Y , YANG M , AI F , et al. Cardioprotective effect of Aloe verabiomacromolecules conjugated with selenium trace element on myocardial ischemia-reperfusion injury in rats[J]. Biol Trace Elem Res, 2017, 177 (2): 345- 352.
24	FORMAN H J , ZHANG H . Targeting oxidative stress in disease: promise and limitations of antioxidant therapy[J]. Nat Rev Drug Discov, 2021, 20 (9): 689- 709.
25	LOBODA A , DAMULEWICZ M , PYZA E , et al. Role of Nrf2/HO-1 system in development, oxidative stress response and diseases: an evolutionarily conserved mechanism[J]. Cell Mol Life Sci, 2016, 73 (17): 3221- 3247.
26	VASCONCELOS A R , DOS SANTOS N B , SCAVONE C , et al. Nrf2/ARE pathway modulation by dietary energy regulation in neurological disorders[J]. Front Pharmacol, 2019, 10, 33.
27	TEBAY L E , ROBERTSON H , DURANT S T , et al. Mechanisms of activation of the transcription factor Nrf2 by redox stressors, nutrient cues, and energy status and the pathways through which it attenuates degenerative disease[J]. Free Radic Biol Med, 2015, 88 (Pt B): 108- 146.
28	周丽娟, 张伟梁, 刘瑞琦, 等. 薯蓣皂苷通过GSK3β/Nrf2/HO-1通路改善尿酸诱导的HK-2细胞氧化应激损伤的作用及机制研究[J]. 中药新药与临床药理, 2024, 35 (3): 342- 348.
	ZHOU L J , ZHANG W L , LIU R Q , et al. Effect and mechanism of dioscin on ameliorating uric acid-induced oxidative stress injury in HK-2 cells through GSK3β/Nrf2/HO-1 pathway[J]. Traditional Chinese Drug Research and Clinical Pharmacology, 2024, 35 (3): 342- 348.
29	李连浩. 添加桑叶黄酮对马运动氧化应激水平的影响及其调控机理的研究[D]. 呼和浩特: 内蒙古农业大学, 2022.
	LI L H. Effects of mulberry leaf flavonoids on oxidative stress statusand its regulation mechanism in exercise horse[D]. Hohhot: Inner Mongolia Agricultural University, 2022. (in Chinese)
30	曹迪. Nrf2-ARE信号通路介导的桑叶黄酮缓解马骨骼肌卫星细胞氧化应激的机理研究[D]. 呼和浩特: 内蒙古农业大学, 2021.
	CAO D. The effects of mulberry leaf flavonoids on oxidative stressof horse skeletal muscle satellite cells associated with Nrf2-ARE signaling pathway[D]. Hohhot: Inner Mongolia Agricultural University, 2021. (in Chinese)
31	WHITE S H , WARREN L K , LI C , et al. Submaximal exercise training improves mitochondrial efficiency in the gluteus medius but not in the triceps brachii of young equine athletes[J]. Sci Rep, 2017, 7 (1): 14389.
32	BRYAN K , MCGIVNEY B A , FARRIES G , et al. Equine skeletal muscle adaptations to exercise and training: evidence of differential regulation of autophagosomal and mitochondrial components[J]. BMC Genomics, 2017, 18 (1): 595.
33	LATHAM C M , GUY C P , WESOLOWSKI L T , et al. Fueling equine performance: importance of mitochondrial phenotype in equine athletes[J]. Anim Front, 2022, 12 (3): 6- 14.
34	MARTÍNEZ-REYES I , CHANDEL N S . Mitochondrial TCA cycle metabolites control physiology and disease[J]. Nat Commun, 2020, 11 (1): 102.
35	LYONS C N , LEARY S C , MOYES C D . Bioenergetic remodeling during cellular differentiation: changes in cytochrome c oxidase regulation do not affect the metabolic phenotype[J]. Biochem Cell Biol, 2004, 82 (3): 391- 399.
36	FOLMES C D , NELSON T J , MARTINEZ-FERNANDEZ A , et al. Somatic oxidative bioenergetics transitions into pluripotency-dependent glycolysis to facilitate nuclear reprogramming[J]. Cell Metab, 2011, 14 (2): 264- 271.
37	RYALL J G , DELL'ORSO S , DERFOUL A , et al. The NAD⁺-dependent SIRT1 deacetylase translates a metabolic switch into regulatory epigenetics in skeletal muscle stem cells[J]. Cell Stem Cell, 2015, 16 (2): 171- 183.
38	HORI S , HIRAMUKI Y , NISHIMURA D , et al. PDH-mediated metabolic flow is critical for skeletal muscle stem cell differentiation and myotube formation during regeneration in mice[J]. FASEB J, 2019, 33 (7): 8094- 8109.
39	KIM K Y , HWANG S K , PARK S Y , et al. L-serine protects mouse hippocampal neuronal HT22 cells against oxidative stress-mediated mitochondrial damage and apoptotic cell death[J]. Free Radic Biol Med, 2019, 141, 447- 460.
40	STURZA A , PAVEL I , ANCUŞA S , et al. Quercetin exerts an inhibitory effect on cellular bioenergetics of the B164A5 murine melanoma cell line[J]. Mol Cell Biochem, 2018, 447 (1-2): 103- 109.
41	JING J , HE Y , LIU Y , et al. Selenoproteins synergistically protect porcine skeletal muscle from oxidative damage via relieving mitochondrial dysfunction and endoplasmic reticulum stress[J]. J Anim Sci Biotechnol, 2023, 14 (1): 79.
42	LEE S , SHIN H S , SHIREMAN P K , et al. Glutathione-peroxidase-1 null muscle progenitor cells are globally defective[J]. Free Radic Biol Med, 2006, 41 (7): 1174- 1184.
43	HIDALGO M , MARCHANT D , QUIDU P , et al. Oxygen modulates the glutathione peroxidase activity during the L6 myoblast early differentiation process[J]. Cell Physiol Biochem, 2014, 33 (1): 67- 77.
44	MRUGALA D , LEATHERWOOD J L , MORRIS E F , et al. Dietary conjugated linoleic acid supplementation alters skeletal muscle mitochondria and antioxidant status in young horses[J]. J Anim Sci, 2021, 99 (2): skab037.
45	OWEN R N , SEMANCHIK P L , LATHAM C M , et al. Elevated dietary selenium rescues mitochondrial capacity impairment induced by decreased vitamin E intake in young exercising horses[J]. J Anim Sci, 2022, 100 (8): skac172.
46	LATHAM C M , DICKSON E C , OWEN R N , et al. Complexed trace mineral supplementation alters antioxidant activities and expression in response to trailer stress in yearling horses in training[J]. Sci Rep, 2021, 11 (1): 7352.
47	HENRY M L , VELEZ-IRIZARRY D , PAGAN J D , et al. The impact of N-acetyl cysteine and coenzyme Q10 supplementation on skeletal muscle antioxidants and proteome in fit Thoroughbred horses[J]. Antioxidants (Basel), 2021, 10 (11): 1739.

抗氧化指标Antioxidant indexes	组别Group
抗氧化指标Antioxidant indexes	对照组Control	损伤组H₂O₂	Sepol 1	Sepol 2	Sepol 3	Sepol 4	Sepol 5	Sepol 6
T-AOC/(mmol·g^-1)	0.88±0.021^g	0.83±0.0029^g	1.37±0.0019^f	1.85±0.024^d	2.18±0.012^b	2.65±0.013^a	1.94±0.026^c	1.75±0.012^e
GPX/(μmol·mg^-1 prot)	317.75±7.82^b	182.23±1.95^c	322.53±9.82^b	330.86±1.85^b	332.20±7.21^b	356.10±8.08^a	325.03±7.22^b	321.69±9.71^b
SOD/(U·mg^-1 prot)	13.68±0.14^b	5.78±0.15^d	9.18±0.32^c	14.30±0.46^b	14.68±0.68^b	17.34±0.68^a	13.34±0.92^b	12.42±0.93^b
CAT/(U·mg^-1 prot)	25.87±0.36^e	16.11±0.33^f	39.19±0.51^d	41.60±0.25^b	45.21±0.48^a	46.14±0.57^a	41.92±1.04^b	39.76±0.47^c
MDA/(nmol·mg^-1 prot)	0.39±0.00075^c	0.78±0.0018^b	0.45±0.030^c	0.42±0.020^c	0.38±0.0004^c	0.23±0.030^d	0.85±0.026^b	1.57±0.11^a

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硒多糖缓解马骨骼肌卫星细胞氧化损伤作用的研究

Effects of Selenium Polysaccharides on Oxidative Damage of Equine Skeletal Muscle Satellite Cell

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