牛结节性皮肤病免疫抗体监测的Meta分析

doi:10.11843/j.issn.0366-6964.2024.08.036

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (8): 3649-3658.doi: 10.11843/j.issn.0366-6964.2024.08.036

牛结节性皮肤病免疫抗体监测的Meta分析

沈鹏¹^,²(), 王艺²^,³, 任炜杰², 杨永春¹, 宋厚辉¹^,*(), 王志亮²^,*()

1. 浙江农林大学动物科技学院·动物医学院，杭州 311300
2. 中国动物卫生与流行病学中心，青岛 266000
3. 南京农业大学动物医学院教育部动物健康与食品安全国际合作联合实验室江苏省外国专家工作室，南京 210095

收稿日期:2023-08-29 出版日期:2024-08-23 发布日期:2024-08-28
通讯作者: 宋厚辉,王志亮 E-mail:shenpeng200011@foxmail.com;songhh@zafu.edu.cn;wangzhiliang@cahec.cn
作者简介:沈鹏(2000-)，男，浙江嘉兴人，硕士生，主要从事畜牧(兽医方向)研究, E-mail：shenpeng200011@foxmail.com
基金资助:
重大外来动物疫病阻断与防控技术研发(2022YFD1800500)

Meta Analysis of Immune Antibody Monitoring for Lumpy Skin Disease

Peng SHEN¹^,²(), Yi WANG²^,³, Weijie REN², Yongchun YANG¹, Houhui SONG¹^,*(), Zhiliang WANG²^,*()

1. College of Animal Science and Technology·College of Veterinary Medicine College, Zhejiang A&F University, Hangzhou 311300, China
2. China Animal Health and Epidemiology Center, Qingdao 266000, China
3. Jiangsu Foreign Expert Workshop/MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China

Received:2023-08-29 Online:2024-08-23 Published:2024-08-28
Contact: Houhui SONG, Zhiliang WANG E-mail:shenpeng200011@foxmail.com;songhh@zafu.edu.cn;wangzhiliang@cahec.cn

摘要/Abstract

摘要：

疫苗接种是预防牛结节性皮肤病(lumpy skin disease, LSD)的重要措施，但是疫苗接种后诱导的体液免疫应答水平参差不齐，目前尚缺乏评价疫苗免疫效果的有效手段。本文使用R软件利用Meta分析对已发表的LSD免疫抗体监测结果进行统计，并进行亚组分析，综合评价免疫抗体监测的意义。通过计算机分别检索中国知网、万方数据知识服务平台、维普中文科技期刊数据库、PubMed、Web of science等中英文数据库，检索时限截止至2023年7月，收集LSD免疫抗体监测的相关文献，根据制定的文献纳入与排除标准，共纳入符合筛选条件的文献24篇，涉及羊痘疫苗的文献共有13篇，涉及LSD疫苗的文献共有13篇，其中2篇文献同时涉及羊痘疫苗和LSD疫苗，包含8 638例样本，其中阳性样本2 782份，结局指标为免疫抗体阳性率。Meta分析结果显示，羊痘疫苗的免疫抗体水平为41%(95% CI: 27%~55%)，LSD疫苗的免疫抗体水平为73%(95% CI: 64%~86%)，各文献间的同质性较低，两种疫苗之间无显著性差异(P=0.59)，与羊痘疫苗接种(P=0.12)和LSD疫苗接种(P=0.077)相关的文献均提示未存在明显发表偏移；按养殖环境和检测方法分类进行亚组分析，不同养殖环境(P=0.84)和检测方法(P=0.40)均无显著性差异，两亚组均非异质性的主要来源。本文结果提示，使用免疫抗体监测方法评价免疫效果存在较大的不确定性，在实际工作中需要综合分析研判。

关键词: 牛结节性皮肤病, 羊痘, 疫苗, 免疫, 抗体监测, Meta分析

Abstract:

Vaccination is an essential measure in preventing lumpy skin disease (LSD). However, the induction of humoral immune responses post-vaccination varies, and there is currently a lack of effective methods to evaluate vaccine immunogenicity. This study employed Meta-analysis using R software to statistically analyze published data on immune antibody monitoring for LSD, including subgroup analyses, to comprehensively assess the significance of immune antibody monitoring. A computer search was conducted in both Chinese and English databases, including CNKI, Wanfang Data, VIP, PubMed, and Web of Science. The search was up to July 2023, collecting relevant studies on immune antibody monitoring for LSD. Based on predetermined inclusion and exclusion criteria, a total of 24 articles were included. Among these, 13 articles were related to sheep/goat pox vaccine, and 13 articles were related to LSD vaccine. Two articles covered both sheep/goat pox and lumpy skin disease vaccines, with a total of 8, 638 samples, including 2, 782 positive samples. The outcome measure was the positive rate of immune antibodies. Meta-analysis results indicated an immune antibody level of 41% (95%CI: 27%~55%) for sheep/goat pox vaccine and 73% (95%CI: 64%~86%) for LSD vaccine. The homogeneity among studies was low, and there was no significant difference between the two vaccines (P=0.59). The literature related to sheep/goat pox vaccination(P=0.12) and LSD vaccination(P=0.077) both suggested no apparent publication bias. Subgroup analysis was based on immunization sites and detection methods, the mesults showed that there were no significant differences between different immunization sites(P=0.84) or detection methods(P=0.40), indicated non-heterogeneity in both subgroups. These findings suggest that there is significant uncertainty in evaluating immune protection effectiveness using immune antibody monitoring methods, comprehensive analysis and judgment will be necessitating in practical applications.

Key words: lumpy skin disease, sheep/goat pox, vaccine, immunization, antibody monitoring, Meta-analysis

中图分类号:

S858.23

沈鹏, 王艺, 任炜杰, 杨永春, 宋厚辉, 王志亮. 牛结节性皮肤病免疫抗体监测的Meta分析[J]. 畜牧兽医学报, 2024, 55(8): 3649-3658.

Peng SHEN, Yi WANG, Weijie REN, Yongchun YANG, Houhui SONG, Zhiliang WANG. Meta Analysis of Immune Antibody Monitoring for Lumpy Skin Disease[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3649-3658.

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作者(年份) Author(year)	疫苗类型¹⁾ Type of vaccine	检测方法 Detection method	ELISA包被抗原 ELISA coated antigen	样本量 Sample size	阳性数 Number of positives	免疫地点²⁾ Immunization site	评分 Score
张爱琼等^[5](2022)	1	ELISA	羊痘病毒	90	67	1	3
王图雅等^[6](2021)	1	ELISA	LSDV	260	140	1	2
赵晓军等^[7](2022)	1	ELISA	LSDV	120	80	1	3
王宇等^[8](2023)	1	VNT	—	20	8	2	3
闭璟珊等^[9](2021)	1	VNT	—	36	19	1	3
任娟等^[10](2022)	1	ELISA	羊痘病毒	5 520	567	1	1
朱真等^[11](2023)	1、2	ELISA	LSDV	95	77	2	3
Okur-Gumusova等^[12](2020)	1	ELISA	羊痘病毒	131	10	1	2
Haegeman等^[13](2021)	2	IPMA	LSDV	35	30	2	3
Safini等^[14](2022)	2	VNT	—	44	40	2	3
Hamdi等^[15](2020)	2	VNT, ELISA	羊痘病毒	126	23	2	3
Wolff等^[16](2020)	2	ELISA	羊痘病毒	12	11	2	3
Es-Sadeqy等^[17](2021)	2	VNT	—	30	29	2	3
Haegeman等^[18](2023)	2	VNT	—	30	14	2	3
Krešić等^[19](2020)	2	ELISA	羊痘病毒	291	250	1	2
Kumar等^[20](2023)	2	VNT	—	658	561	1, 2	2
Gari等^[21](2015)	2	IFAT	—	30	7	2	3
Hamdi等^[22](2020)	1、2	VNT	—	21	7	2	3
Milovanović等^[23](2019)	2	VNT	—	156	87	1	3
Sareyyupoĝlu等^[24](2023)	1	ELISA	LSDV	10	8	2	3
Bamouh等^[25](2021)	2	VNT	—	69	50	2	3
Abutarbush和Tuppurainen^[26](2018)	1	VNT	—	51	8	2	3
Hakobyan等^[27](2023)	1	ELISA	羊痘病毒	798	687	1	2
Burkov等^[28](2020)	1	ELISA	羊痘病毒	5	2	2	3

免疫环境 Immunization site	羊痘疫苗Sheep/goat pox vaccine			LSD疫苗LSD vaccine
免疫环境 Immunization site	样本量 Sample size	异质性 Heterogeneity (I²)	免疫抗体水平 Immune antibody levels (95%CI)	样本量 Sample size	异质性 Heterogeneity (I²)	免疫抗体水平 Immune antibody levels (95%CI)
自然环境 Natural environment	6 955	99.8%	43%(23%~63%)	1 186	95.5%	75%(58%~87%)
实验室环境 Laboratory environment	137	97.8%	39%(18%~59%)	360	84.1%	74%(59%~86%)

检测方法 Detection method	羊痘疫苗Sheep/Goat pox vaccine			LSD疫苗LSD vaccine
检测方法 Detection method	样本量 Sample size (n)	异质性 Heterogeneity (I²)	免疫抗体水平 Immune antibody levels (95%CI)	样本量 Sample size (n)	异质性 Heterogeneity (I²)	免疫抗体水平 Immune antibody levels (95%CI)
VNT	113	75.3%	26%(11%~40%)	1 037	93.7%	72%(58%~85%)
ELISA	6 938	99.7%	51%(24%~77%)	557	97.2%	69%(57%~82%)

包被抗原 ELISA coated antigen	羊痘疫苗Sheep/Goat pox vaccine			LSD疫苗LSD vaccine
包被抗原 ELISA coated antigen	样本量 Sample size (n)	异质性 Heterogeneity (I²)	免疫抗体水平 Immune antibody levels (95%CI)	样本量 Sample size (n)	异质性 Heterogeneity (I²)	免疫抗体水平 Immune antibody levels (95%CI)
羊痘病毒	6 544	99.9%	34%(7%~60%)	394	33.1%	85%(82%~89%)
LSDV	435	98.1%	73%(55%~92%)	—	—	—

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牛结节性皮肤病免疫抗体监测的Meta分析

Meta Analysis of Immune Antibody Monitoring for Lumpy Skin Disease

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