艾美耳球虫的遗传操作：平台建立、应用与展望

doi:10.11843/j.issn.0366-6964.2024.08.010

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (8): 3362-3373.doi: 10.11843/j.issn.0366-6964.2024.08.010

艾美耳球虫的遗传操作：平台建立、应用与展望

梁瑞英¹(), 索静霞²(), 梁琳¹, 刘贤勇², 丁家波¹, 索勋², 汤新明¹^,*()

1. 中国农业科学院北京畜牧兽医研究所农业农村部动物生物安全风险预警及防控重点实验室(北方)农业农村部兽用生物制品与化学药品重点实验室, 北京 100193
2. 中国农业大学动物医学院兽医公共卫生安全全国重点实验室农业农村部国家动物寄生原虫实验室, 北京 100193

收稿日期:2024-03-20 出版日期:2024-08-23 发布日期:2024-08-28
通讯作者: 汤新明 E-mail:liangruiying@caas.cn;suojingxia415@126.com;tangxinming@caas.cn
作者简介:梁瑞英(1989-)，女，山东菏泽人，助研助理研究员，主要从事动物疫苗及分子免疫学研究，E-mail：liangruiying@caas.cn
索静霞(1989-)，女，河北张家口人，助理兽医师，主要从事寄生虫遗传学与免疫学研究，E-mail：suojingxia415@126.com
第一联系人：
梁瑞英和索静霞为同等贡献作者
基金资助:
国家自然科学基金(32273035);国家自然科学基金(31902295);中国农业科学院青年创新专项(Y2023QC10)

Genetic Manipulation of Eimeria: Platform Development, Application, and Perspective

Ruiying LIANG¹(), Jingxia SUO²(), Lin LIANG¹, Xianyong LIU², Jiabo DING¹, Xun SUO², Xinming TANG¹^,*()

1. Key Laboratory of Animal Biosafety Risk Prevention and Control (North) & Key Laboratory of Veterinary Biological Products and Chemical Drugs of MARA, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2. National Key Laboratory of Veterinary Public Health Security, National Animal Protozoa Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China

Received:2024-03-20 Online:2024-08-23 Published:2024-08-28
Contact: Xinming TANG E-mail:liangruiying@caas.cn;suojingxia415@126.com;tangxinming@caas.cn

摘要/Abstract

摘要：

艾美耳属球虫是畜牧生产中最常见的寄生虫，引起鸡、兔、羊、牛等养殖动物严重消化系统疾病，影响动物健康和养殖效益。随着分子生物学技术的飞速发展，研究者们先后建立了艾美耳球虫的瞬时转染、稳定筛选和基因编辑等遗传操作平台，实现了艾美耳球虫的基因过表达、基因敲除和基因编辑等操作，极大推进了艾美耳球虫基础生物学和作为新型真核载体疫苗的研究进展，但仍面临一些挑战。本文围绕艾美耳球虫遗传操作平台建立的关键技术、创新性设计、存在的技术瓶颈以及应用前景进行系统梳理和总结，为其他畜禽寄生虫遗传操作平台的构建提供参考，也为球虫病新型防控制剂的研制提供新的思路。

关键词: 艾美耳球虫, 遗传操作, 基因编辑, 技术瓶颈, 疫苗载体

Abstract:

The genus of Eimeria parasite is the most common parasite in poultry and livestock, causing severe digestive system diseases in animals such as chickens, rabbits, sheep, and cattle, which affect animal health and the economic benefits of animal farming. With the rapid development of molecular biology technologies, researchers have established genetic manipulation platform for Eimeria, including transient transfection, stable selection, and gene editing. These techniques have realized gene overexpression, gene knockout, and gene editing in Eimeria, greatly advancing the understanding of Eimeria's basic biology and its potential as a novel eukaryotic vector vaccine. However, challenges still remain in this field. This article systematically reviews and summarizes the key technologies, innovative designs, technical bottlenecks, and application prospects of Eimeria's genetic manipulation platform, providing references for the establishment of genetic manipulation platform for other livestock parasites and offering new insights for the development of new control measures against coccidiosis.

Key words: Eimeria spp, genetic manipulation, gene editing, technical bottleneck, vaccine vector

中图分类号:

S852.723

梁瑞英, 索静霞, 梁琳, 刘贤勇, 丁家波, 索勋, 汤新明. 艾美耳球虫的遗传操作：平台建立、应用与展望[J]. 畜牧兽医学报, 2024, 55(8): 3362-3373.

Ruiying LIANG, Jingxia SUO, Lin LIANG, Xianyong LIU, Jiabo DING, Xun SUO, Xinming TANG. Genetic Manipulation of Eimeria: Platform Development, Application, and Perspective[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3362-3373.

图/表 2

图 1

表 1

基于不同受体细胞类型的艾美耳球虫瞬时转染与稳定筛选"

受体细胞类型 Recipient Cell	虫种名称 Eimeria spp	宿主 Host	报告基因 Reporter	接种方式 Inoculation Routes	瞬时表达 Transient Expression	稳定表达 Stable Expression	参考文献
子孢子 Sporozoite	柔嫩艾美耳球虫 E. tenella	鸡 Chicken	β-半乳糖苷酶 β-galactosidase	—	+	—	[37]
		鸡 Chicken	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	泄殖腔 Cloacal inoculation	+	+	[10, 29, 32]
	和缓艾美耳球虫 E. mitis	鸡 Chicken	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	泄殖腔 Cloacal inoculation	+	+	[14, 39]
	堆型艾美耳球虫 E. acervulina	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	翅下静脉 Intravenous inoculation	+	+	[19, 40]
	大型艾美耳球虫 E. magna	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	手术接种 Surgical inoculation	+	+	[15]
	肠艾美耳球虫 E. intestinalis	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	手术接种 Surgical inoculation	+	+	[16]
	中型艾美耳球虫 E. media	兔 Rabbit	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	手术接种 Surgical inoculation	—	+	未发表数据
	斯氏艾美耳球虫 E. stiedai	小鼠 Mouse	荧光蛋白 Fluorescent Protein	静脉接种 Intravenous inoculation	—	+	未发表数据
	镰型艾美耳球虫 E. falciformis	大鼠 Rat	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	经口接种 Oral inoculation	—	+	[18, 41]
	尼氏艾美耳球虫 E. nieschulzi		荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	经口接种 Oral inoculation	+	+	[30]
裂殖子 Merozoite	毒害艾美耳球虫 E. necatrix	鸡 Chicken	荧光蛋白/抗性基因 Fluorescent Protein/ Drug Resistance Gene	泄殖腔 Cloacal inoculation	—	+	[13, 42]
孢子囊 Sporocyst	镰型艾美耳球虫 E. falciformis	小鼠 Mouse	荧光蛋白/抗性基因 Fluorescent Protein/Drug Resistance Gene	经口接种 Oral inoculation	—	+	未发表数据
卵囊 Oocyst	巨型艾美耳球虫 E. maxima	鸡 Chicken	荧光蛋白 Fluorescent Protein	—	—	—	[43]

表 1

参考文献 73

1	LIUQ,LIUX Y,ZHAOX M,et al.Live attenuated anticoccidial vaccines for chickens[J].Trends Parasitol,2023,39(12):1087-1099. doi: 10.1016/j.pt.2023.09.002
2	GAOY,SUNP,HUD D,et al.Advancements in understanding chicken coccidiosis: from Eimeria biology to innovative control strategies[J].One Health Adv,2024,2(1):6. doi: 10.1186/s44280-024-00039-x
3	BLAKED P,KNOXJ,DEHAECKB,et al.Re-calculating the cost of coccidiosis in chickens[J].Vet Res,2020,51(1):115. doi: 10.1186/s13567-020-00837-2
4	CHAPMANH D.Milestones in avian coccidiosis research: a review[J].Poult Sci,2014,93(3):501-511. doi: 10.3382/ps.2013-03634
5	CHAPMANH D,BARTAJ R,BLAKED,et al.A selective review of advances in coccidiosis research[J].Adv Parasitol,2013,83,93-171.
6	WANGS,SUOX.Still naïve or primed: Anticoccidial vaccines call for memory[J].Exp Parasitol,2020,216,107945. doi: 10.1016/j.exppara.2020.107945
7	REIDA J,BLAKED P,ANSARIH R,et al.Genomic analysis of the causative agents of coccidiosis in domestic chickens[J].Genome Res,2014,24(10):1676-1685. doi: 10.1101/gr.168955.113
8	TANGX M,LIUX Y,SUOX.Towards innovative design and application of recombinant Eimeria as a vaccine vector[J].Infect Immun,2020,88(5):e00861-19.
9	PASTOR-FERNÁNDEZI,PEGGE,MACDONALDS E,et al.Laboratory growth and genetic manipulation of Eimeria tenella[J].Curr Protoc Microbiol,2019,53(1):e81. doi: 10.1002/cpmc.81
10	HAOL L,LIUX Y,ZHOUX Y,et al.Transient transfection of Eimeria tenella using yellow or red fluorescent protein as a marker[J].Mol Biochem Parasit,2007,153(2):213-215. doi: 10.1016/j.molbiopara.2007.02.005
11	TANGX M,LIUX Y,TAOG R,et al."Self-cleaving" 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella[J].Vet Res,2016,47(1):68. doi: 10.1186/s13567-016-0351-z
12	YUY,TANGX M,DUANC H,et al.Microneme-located VP2 in Eimeria acervulina elicits effective protective immunity against infectious bursal disease virus[J].Infect Immun,2024,92(2):e0045623. doi: 10.1128/iai.00456-23
13	DUANC H,HUD D,TANGX M,et al.Stable transfection of Eimeria necatrix through nucleofection of second generation merozoites[J].Mol Biochem Parasit,2019,228,1-5. doi: 10.1016/j.molbiopara.2019.01.002
14	LIZ R,TANGX M,SUOJ X,et al.Transgenic Eimeria mitis expressing chicken interleukin 2 stimulated higher cellular immune response in chickens compared with the wild-type parasites[J].Front Microbiol,2015,6,533.
15	TAOG R,SHIT Y,TANGX M,et al.Transgenic Eimeria magna Pérard, 1925 displays similar parasitological properties to the wild-type strain and induces an exogenous protein-specific immune response in rabbits (Oryctolagus cuniculus L.)[J].Front Immunol,2017,8,2.
16	SHIT Y,TAOG R,BAOG L,et al.Stable transfection of Eimeria intestinalis and investigation of its life cycle, reproduction and immunogenicity[J].Front Microbiol,2016,7,807.
17	KURTHM,ENTZEROTHR.Reporter gene expression in cell culture stages and oocysts of Eimeria nieschulzi (Coccidia, Apicomplexa)[J].Parasitol Res,2009,104(2):303-310. doi: 10.1007/s00436-008-1192-0
18	SHIF Y,ZHANGS X,ZHANGN,et al.Tissue-resident, memory CD8⁺ T cells are effective in clearing intestinal Eimeria falciformis reinfection in mice[J].Front Immunol,2023,14,1128637. doi: 10.3389/fimmu.2023.1128637
19	ZHANGS X,TANGX M,WANGS,et al.Establishment of recombinant Eimeria acervulina expressing multi-copies M2e derived from avian influenza virus H9N2[J].Vaccines (Basel),2021,9(7):791. doi: 10.3390/vaccines9070791
20	ZOUJ,LIUX Y,SHIT Y,et al.Transfection of Eimeria and Toxoplasma using heterologous regulatory sequences[J].Int J Parasitol,2009,39(11):1189-1193. doi: 10.1016/j.ijpara.2009.03.006
21	TANGX M,LIUX Y,YING W,et al.A novel vaccine delivery model of the apicomplexan Eimeria tenella expressing Eimeria maxima antigen protects chickens against infection of the two parasites[J].Front Immunol,2018,8,1982. doi: 10.3389/fimmu.2017.01982
22	YING W,LIUX Y,ZOUJ,et al.Co-expression of reporter genes in the widespread pathogen Eimeria tenella using a double-cassette expression vector strategy[J].Int J Parasitol,2011,41(8):813-816. doi: 10.1016/j.ijpara.2011.04.001
23	SHIT Y,YANW C,RENH B,et al.Dynamic development of parasitophorous vacuole of Eimeria tenella transfected with the yellow fluorescent protein gene fused to different signal sequences from apicomplexan parasites[J].Parasitol Res,2009,104(2):315-320. doi: 10.1007/s00436-008-1194-y
24	YING W,QINM,LIUX Y,et al.Expression of Toxoplasma gondii dense granule protein7 (GRA7) in Eimeria tenella[J].Parasitol Res,2013,112(5):2105-2109. doi: 10.1007/s00436-013-3307-5
25	YUY,ZHANGS X,DUANC H,et al.Developing efficient strategies for localizing the enhanced yellow fluorescent protein subcellularly in transgenic Eimeria parasites[J].Sci Rep,2024,14(1):4851. doi: 10.1038/s41598-024-55569-6
26	MARUGAN-HERNANDEZV,LONGE,BLAKED,et al.Eimeria tenella protein trafficking: differential regulation of secretion versus surface tethering during the life cycle[J].Sci Rep,2017,7,4557. doi: 10.1038/s41598-017-04049-1
27	THATHY V, MÉNARD R. Gene targeting in Plasmodium berghei[M]//DOOLAN D L. Malaria Methods and Protocols. Totowa: Humana Press, 2002, 72: 317-331.
28	MARUGAN-HERNANDEZV,COCKLEC,MACDONALDS,et al.Viral proteins expressed in the protozoan parasite Eimeria tenella are detected by the chicken immune system[J].Parasit Vectors,2016,9(1):463. doi: 10.1186/s13071-016-1756-2
29	YANW C,LIUX Y,SHIT Y,et al.Stable transfection of Eimeria tenella: Constitutive expression of the YFP-YFP molecule throughout the life cycle[J].Int J Parasitol,2009,39(1):109-117. doi: 10.1016/j.ijpara.2008.06.013
30	HANIGS,ENTZEROTHR,KURTHM.Chimeric fluorescent reporter as a tool for generation of transgenic Eimeria (Apicomplexa, Coccidia) strains with stage specific reporter gene expression[J].Parasitol Int,2012,61(3):391-398. doi: 10.1016/j.parint.2012.01.010
31	SUNP,WANGC Y,ZHANGY Y,et al.Transcriptome profile of halofuginone resistant and sensitive strains of Eimeria tenella[J].Front Microbiol,2023,14,1141952. doi: 10.3389/fmicb.2023.1141952
32	SUNP,ZHANGY Y,WANGC Y,et al.EtcPRS^Mut as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii[J].iScience,2023,26(4):106334. doi: 10.1016/j.isci.2023.106334
33	HAOZ K,CHENJ M,SUNP,et al.Distinct non-synonymous mutations in cytochrome b highly correlate with decoquinate resistance in apicomplexan parasite Eimeria tenella[J].Parasit Vectors,2023,16(1):365. doi: 10.1186/s13071-023-05988-7
34	ZHANGH T,ZHANGL,RENG L,et al.Forward genetic analysis of monensin and diclazuril resistance in Eimeria tenella[J].Int J Parasitol Drugs Drug Resist,2023,22,44-51. doi: 10.1016/j.ijpddr.2023.05.002
35	毕菲菲,郝振凯,孙霈,等.基于全基因组重测序及等位基因频率解析柔嫩艾美耳球虫对地克珠利耐药分子机制的初步研究[J].中国兽医杂志,2022,58(8):1-6.
	BIF F,HAOZ K,SUNP,et al.Preliminary study of molecular mechanisms of diclazuril resistance in Eimeria tenella based on whole-genome resequencing and allele frequency analysis[J].Chinese Journal of Veterinary Medicine,2022,58(8):1-6.
36	GOONEWARDENER,DAILYJ,KASLOWD,et al.Transfection of the malaria parasite and expression of firefly luciferase[J].Proc Natl Acad Sci U S A,1993,90(11):5234-5236. doi: 10.1073/pnas.90.11.5234
37	KELLEHERM,TOMLEYF M.Transient expression of β-galactosidase in differentiating sporozoites of Eimeria tenella[J].Mol Biochem Parasitol,1998,97(1-2):21-31. doi: 10.1016/S0166-6851(98)00128-5
38	闫文朝,王天奇,索勋,等.表达黄色荧光蛋白和乙胺嘧啶抗性基因的转基因柔嫩艾美耳球虫的发育和致病性分析[J].畜牧兽医学报,2009,40(12):1794-1798.
	YANW C,WANGT Q,SUOX,et al.Analysis for pathogenicity and development of transgenic Eimeria tenella expressing both yellow fluorescent protein and TgDHFR-TS genes[J].Acta Veterinaria et Zootechnica Sinica,2009,40(12):1794-1798.
39	QINM,LIUX Y,TANGX M,et al.Transfection of Eimeria mitis with yellow fluorescent protein as reporter and the endogenous development of the transgenic parasite[J].PLoS ONE,2014,9(12):e114188. doi: 10.1371/journal.pone.0114188
40	LIUJ,SHIF Y,ZHANGY Y,et al.Evidence of high-efficiency cross fertilization in Eimeria acervulina revealed using two lines of transgenic parasites[J].Int J Parasitol,2023,53(2):81-89. doi: 10.1016/j.ijpara.2022.10.007
41	HUANGG P,ZHANGS X,ZHOUC X,et al.Influence of Eimeria falciformis infection on gut microbiota and metabolic pathways in mice[J].Infect Immun,2018,86(5):e00073.
42	DUAN C H, TANG X M, HU D D, et al. Nucleofection and in vivo propagation of chicken Eimeria parasites[J/OL]. J Vis Exp, 2020(156): doi: 10.3791/60552.[2024-06-02]. https://www.jove.com/cn/t/60552/nucleofection-vivo-propagation-chicken-eimeria-parasites.
43	LIJ N,ZOUJ,YING W,et al.Plasmid DNA could be delivered into Eimeria maxima unsporulated oocyst with gene gun system[J].Acta Vet Hung,2012,60(4):431-440. doi: 10.1556/avet.2012.037
44	赵世云,余芳,刘贤勇,等.电穿孔转染对柔嫩艾美耳球虫卵囊孢子化率影响的观察[J].中国兽医杂志,2010,46(10):31-32.
	ZHAOS Y,YUF,LIUX Y,et al.Effect of electroporation transfection on sporulation rate of Eimeria tenella oocyst[J].Chinese Journal of Veterinary Medicine,2010,46(10):31-32.
45	JONSCHERE,ERDBEERA,GVNTHERM,et al.Two COWP-like cysteine rich proteins from Eimeria nieschulzi (coccidia, apicomplexa) are expressed during sporulation and involved in the sporocyst wall formation[J].Parasit Vectors,2015,8,395. doi: 10.1186/s13071-015-0982-3
46	钱锋,肖成组.电击缓冲液对哺乳动物细胞电穿孔效率的影响[J].军事医学科学院院刊,1999,23(2):101-103.
	QIANF,XIAOC Z.Effect of electroporation buffers on gene transfer efficiency of mammalian cells[J].Bulletin of the Academy of Military Medical Sciences,1999,23(2):101-103.
47	闫文朝,王天奇,索勋,等.转染技术在顶复门原虫研究中的应用[J].中国农业科学,2009,42(10):3693-3699.
	YANW C,WANGT Q,SUOX,et al.Transfection technology and its application in the investigation of apicomplexan protozoa[J].Scientia Agricultura Sinica,2009,42(10):3693-3699.
48	赵世云,苏华荔,刘贤勇,等.柔嫩艾美耳球虫电穿孔转染条件的优化[J].中国畜牧兽医,2010,37(11):53-56.
	ZHAOS Y,SUH L,LIUX Y,et al.Optimization of electroporation for transfection of Eimeria tenella[J].China Animal Husbandry & Veterinary Medicine,2010,37(11):53-56.
49	LIUX Y,SHIT Y,RENH B,et al.Restriction enzyme-mediated transfection improved transfection efficiency in vitro in Apicomplexan parasite Eimeria tenella[J].Mol Biochem Parasit,2008,161(1):72-75. doi: 10.1016/j.molbiopara.2008.06.006
50	CLARKJ D,BILLINGTONK,BUMSTEADJ M,et al.A toolbox facilitating stable transfection of Eimeria species[J].Mol Biochem Parasitol,2008,162(1):77-86. doi: 10.1016/j.molbiopara.2008.07.006
51	SHIT Y,LIUX Y,HAOL L,et al.Transfected Eimeria tenella could complete its endogenous development in vitro[J].J Parasitol,2008,94(4):978-980. doi: 10.1645/GE-1412.1
52	SUH L,LIUX Y,YANW C,et al.piggyBac transposon-mediated transgenesis in the apicomplexan parasite Eimeria tenella[J].PLoS ONE,2012,7(6):e40075. doi: 10.1371/journal.pone.0040075
53	LIUX Y,ZOUJ,YING W,et al.Development of transgenic lines of Eimeria tenella expressing M2e-enhanced yellow fluorescent protein (M2e-EYFP)[J].Vet Parasitol,2013,193(1-3):1-7. doi: 10.1016/j.vetpar.2012.12.019
54	郝振凯,汤新明,张媛媛,等.利用二代测序技术对柔嫩艾美耳球虫T-DNA整合位点的分析及鉴定[J].中国农业大学学报,2023,28(8):183-190.
	HAOZ K,TANGX M,ZHANGY Y,et al.Analysis and identification of T-DNA insertion sites in transgenic Eimeria tenella[J].Journal of China Agricultural University,2023,28(8):183-190.
55	HUD D,TANGX M,BEN MAMOUNC,et al.Efficient single-gene and gene family editing in the apicomplexan parasite Eimeria tenella using CRISPR-Cas9[J].Front Bioeng Biotechnol,2020,8,128. doi: 10.3389/fbioe.2020.00128
56	TANGX M,SUOJ X,LIANGL,et al.Genetic modification of the protozoan Eimeria tenella using the CRISPR/Cas9 system[J].Vet Res,2020,51(1):41. doi: 10.1186/s13567-020-00766-0
57	罗伏兵,徐发荣,王慧强,等.夹心ELISA法测定基因重组球虫表达异源蛋白的水平[J].中国兽医杂志,2020,56(8):36-38.
	LUOF B,XUF R,WANGH Q,et al.The heterologous protein expression level in recombinant Eimeria was detected by sandwich ELISA[J].Chinese Journal of Veterinary Medicine,2020,56(8):36-38.
58	QINM,TANGX M,YING W,et al.Chicken IgY Fc expressed by Eimeria mitis enhances the immunogenicity of E. mitis[J].Parasit Vectors,2016,9,164. doi: 10.1186/s13071-016-1451-3
59	CHENGP P,ZHANGZ H,YANGF Y,et al.FnCas12a/crRNA-mediated genome editing in Eimeria tenella[J].Front Genet,2021,12,738746. doi: 10.3389/fgene.2021.738746
60	CHENL L,TANGX M,SUNP,et al.Comparative transcriptome profiling of Eimeria tenella in various developmental stages and functional analysis of an ApiAP2 transcription factor exclusively expressed during sporogony[J].Parasit Vectors,2023,16(1):241. doi: 10.1186/s13071-023-05828-8
61	ZHAOH Z,DONGH,ZHAOQ P,et al.Integrated application of transcriptomics and metabolomics provides insight into the mechanism of Eimeria tenella resistance to maduramycin[J].Int J Parasitol Drugs Drug Resist,2024,24,100526. doi: 10.1016/j.ijpddr.2024.100526
62	SONGH Y,DENGM L,YANGJ F,et al.Transcriptomic, 16S ribosomal ribonucleic acid and network pharmacology analyses shed light on the anticoccidial mechanism of green tea polyphenols against Eimeria tenella infection in Wuliangshan black-boned chickens[J].Parasit Vectors,2023,16(1):330. doi: 10.1186/s13071-023-05922-x
63	TANGX M,SUOJ X,LIC,et al.Transgenic Eimeria tenella expressing profilin of Eimeria maxima elicits enhanced protective immunity and alters gut microbiome of chickens[J].Infect Immun,2018,86(9):e00888-17.
64	HUANGX X,ZOUJ,XUH Q,et al.Transgenic Eimeria tenella expressing enhanced yellow fluorescent protein targeted to different cellular compartments stimulated dichotomic immune responses in chickens[J].J Immunol,2011,187(7):3595-3602. doi: 10.4049/jimmunol.1100043
65	TANGX M,WANGC Y,LIANGL,et al.Co-immunization with two recombinant Eimeria tenella lines expressing immunoprotective antigens of E. maxima elicits enhanced protection against E. maxima infection[J].Parasit Vectors,2019,12(1):347. doi: 10.1186/s13071-019-3605-6
66	PASTOR-FERNÁNDEZI,KIMS,BILLINGTONK,et al.Development of cross-protective Eimeria-vectored vaccines based on apical membrane antigens[J].Int J Parasitol,2018,48(7):505-518. doi: 10.1016/j.ijpara.2018.01.003
67	PASTOR-FERNÁNDEZI,KIMS,MARUGÁN-HERNÁNDEZV,et al.Vaccination with transgenic Eimeria tenella expressing Eimeria maxima AMA1 and IMP1 confers partial protection against high-level E. maxima challenge in a broiler model of coccidiosis[J].Parasit Vectors,2020,13(1):343. doi: 10.1186/s13071-020-04210-2
68	TANGX M,YING W,QINM,et al.Transgenic Eimeria tenella as a vaccine vehicle: expressing TgSAG1 elicits protective immunity against Toxoplasma gondii infections in chickens and mice[J].Sci Rep,2016,6,29379. doi: 10.1038/srep29379
69	CLARKJ D,OAKESR D,REDHEADK,et al.Eimeria species parasites as novel vaccine delivery vectors: Anti-Campylobacter jejuni protective immunity induced by Eimeria tenella-delivered CjaA[J].Vaccine,2012,30(16):2683-2688. doi: 10.1016/j.vaccine.2012.02.002
70	FEIXA S,CRUZ-BUSTOST,RUTTKOWSKIB,et al.Progression of asexual to sexual stages of Cystoisospora suis in a host cell-free environment as a model for Coccidia[J].Parasitology,2021,148(12):1475-1481. doi: 10.1017/S0031182021001074
71	YUY,HUANGW H,WANGQ J,et al.Molecular characterization and analysis of drug resistance-associated protein enolase 2 of Eimeria tenella[J].Int J Parasitol Drugs Drug Resist,2023,21,81-90. doi: 10.1016/j.ijpddr.2023.01.004
72	MATSUBAYASHIM,YAMAGUCHIH,HATTAT,et al.Transitions in morphological forms and rapid development of the asexual schizonts of Eimeria tenella through serial passaging in chicks[J].Infect Genet Evol,2019,75,103993. doi: 10.1016/j.meegid.2019.103993
73	FANGS F,GUX L,EL-ASHRAMS,et al.Immune protection provided by a precocious line trivalent vaccine against rabbit Eimeria[J].Vet Parasitol,2019,275,108927. doi: 10.1016/j.vetpar.2019.108927

[1]	刘雯雯, 董发明, 毕延震. 多基因编辑技术的发展及其在畜牧种质创新中的应用[J]. 畜牧兽医学报, 2024, 55(8): 3267-3275.
[2]	陈浩, 郝哥, 蒲家艳, 肖洁, 熊常明, 何维, 朱煜华, 许力文, 姜庆, 杨光友. 肠艾美耳球虫重组微线蛋白2对家兔免疫保护效果评价[J]. 畜牧兽医学报, 2024, 55(6): 2588-2598.
[3]	周思含, 李天恩, 邓金华, 孙洪超, 闫文朝, 石团员, 王天奇. 浙江省部分地区鸡球虫感染情况调查分析[J]. 畜牧兽医学报, 2024, 55(6): 2629-2640.
[4]	王乐乐, 王礼跃, 蔡为民, 康喜龙, 冯茜茜, 张知之, 范雪莲, 朱玉, 刘丹丹, 许金俊, 潘志明, 陶建平. 鸡球虫重组蛋白rEnApiAP2对鸡免疫保护效果的观察[J]. 畜牧兽医学报, 2024, 55(4): 1716-1727.
[5]	高龙, 常心怡, 李程, 赵晓亚, 李汶洁, 范浩谦, 马静云. 表达外源基因SPAM1重组CAV-2溶瘤病毒的构建与拯救[J]. 畜牧兽医学报, 2024, 55(3): 1228-1237.
[6]	王贺, 郭志廷, 李建喜, 张景艳, 王磊, 张康, 孙继文, 尚小粉, 马永华. 常山散对雏鸡感染柔嫩艾美耳球虫不同发育阶段的防治效果[J]. 畜牧兽医学报, 2024, 55(3): 1278-1289.
[7]	彭月梅, 叶状, 汪飞燕, 王礼跃, 冯永翠, 王乐乐, 候照峰, 许金俊, 陶建平, 刘丹丹. 毒害艾美耳球虫谷胱甘肽过氧化物酶EnGPX的原核表达与分析[J]. 畜牧兽医学报, 2024, 55(2): 846-853.
[8]	张晨俭, 李隐侠, 丁强, 刘伟佳, 王慧利, 何南, 吴家顺, 曹少先. CRISPR/Cas9技术高效制备山羊SOCS2基因编辑胚胎[J]. 畜牧兽医学报, 2024, 55(1): 129-141.
[9]	陈曦, 王一, 王佳丽, 杨新, 宋军科, 赵光辉. 毒害艾美耳球虫和产气荚膜梭菌双重PCR检测方法的建立[J]. 畜牧兽医学报, 2023, 54(9): 3985-3990.
[10]	郑若愚, 任永军, 肖洁, 白鑫, 蒲家艳, 陈浩, 杨光友. 斯氏艾美耳球虫3-磷酸甘油醛脱氢酶重组蛋白对兔的免疫保护效果评价[J]. 畜牧兽医学报, 2023, 54(6): 2581-2595.
[11]	陈芳芳, 栗中华, 朱志伟, 李锦春, 刘翠艳. 恒定链的多功能研究新进展[J]. 畜牧兽医学报, 2023, 54(5): 1824-1833.
[12]	刘铃, 王丹丹, 崔凯, 马月辉, 蒋琳. 猪繁殖与呼吸综合征抗病育种研究进展[J]. 畜牧兽医学报, 2023, 54(2): 434-442.
[13]	张硕, 周雨潇, 吴海波, 索伦. 长效CRISPR/Cas9基因编辑结局的动态追踪研究[J]. 畜牧兽医学报, 2023, 54(10): 4196-4208.
[14]	肖洁, 罗跃军, 陈浩, 蒲家艳, 何维, 熊常明, 郝哥, 任永军, 杨光友. 兔斯氏艾美耳球虫、肠艾美耳球虫和大型艾美耳球虫PCR检测靶标的筛选与单一、多重直接PCR检测方法的建立[J]. 畜牧兽医学报, 2023, 54(10): 4327-4337.
[15]	郑若愚, 肖洁, 白鑫, 陈浩, 蒲家艳, 任永军, 杨光友. 大型艾美耳球虫重组蛋白GAPDH对兔的免疫保护效果评价[J]. 畜牧兽医学报, 2023, 54(10): 4338-4349.

艾美耳球虫的遗传操作：平台建立、应用与展望

Genetic Manipulation of Eimeria: Platform Development, Application, and Perspective

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 2

参考文献 73

相关文章 15

编辑推荐

Metrics

本文评价