畜牧兽医学报 ›› 2023, Vol. 54 ›› Issue (8): 3393-3405.doi: 10.11843/j.issn.0366-6964.2023.08.024

• 营养与饲料 • 上一篇    下一篇

不同运动量对滩羊瘤胃菌群多样性的影响

马友记1*, 陈鹏飞1, 马青2, 吴怡1   

  1. 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 宁夏农林科学院动物科学研究所, 银川 750002
  • 收稿日期:2022-12-05 出版日期:2023-08-23 发布日期:2023-08-22
  • 通讯作者: 马友记,主要从事羊生产学研究,E-mail:yjma@gsau.edu.cn
  • 作者简介:马友记(1976-),男,山东汶上人,教授,博士,主要从事羊生产学研究,E-mail:yjma@gsau.edu.cn
  • 基金资助:
    甘肃省教育厅双一流科研重点项目(GSSYLXM-02);甘肃农业大学学科团队项目(GAU-XKTD-2022-20)

Effects of Different Exercise Amounts on Rumen Microflora Diversity of Tan Sheep

MA Youji1*, CHEN Pengfei1, MA Qing2, WU Yi1   

  1. 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China
  • Received:2022-12-05 Online:2023-08-23 Published:2023-08-22

摘要: 旨在探究不同运动量对滩羊瘤胃菌落多样性的影响。选取60只体况良好、体重(20±0.5) kg的滩羊断奶羔羊,随机分为3组:1)对照组(NC组),全舍饲饲养;2)5公里组(5 km组),每日沿生态牧场运动围栏运动5公里;3)10公里组(10 km组),每日沿生态牧场运动围栏运动10公里。每组5个重复,每个重复4只羊。试验期130 d,其中预试期10 d,正试期120 d。试验结束后,采集瘤胃内容物进行宏基因组高通量测序以测定瘤胃微生物区系的多样性。结果显示:1)5 km组和10 km组的Shannon、Simpson和Chao1指数均高于NC组(P>0.05);2)物种注释发现,拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)占比最高,但两者的相对丰度在试验组和NC组差异均不显著(P>0.05),试验组的瘤胃球菌属(Ruminococcus)和乳杆菌属(Lactobacillus)相对丰度均显著高于NC组(P<0.05)。综上所述,在本试验条件下,增加运动量能够增加滩羊瘤胃微生物的丰富度和多样性,改变部分菌属的相对丰富度,但对瘤胃微生物的均匀度影响不显著。

关键词: 滩羊, 运动, 宏基因组学, 瘤胃, 菌群

Abstract: This experiment aimed to explore the effects of different exercise amounts on rumen microflora of Tan sheep. Sixty Tan sheep weaned lambs (body weight (20±0.5) kg) with good body condition were randomly divided into three groups:1) Control group (NC group), whole barn feeding; 2) 5 kilometers group (5 km group), daily exercise along the ecological pasture fence 5 km; 3) 10 kilometers group (10 km group), daily exercise along the ecological pasture fence 10 km. The are 5 replicates per group and 4 sheep in each replicate. The experiment lasted for 130 days, including 10 days of pre-experiment and 120 days of formal experiment. At the end of the experiment, rumen contents were collected for metagenomic sequencing to determine the diversity of rumen microflora. Results were showed as follows:1) The Shannon, Simpson and Chao1 indexes of 5 km and 10 km groups were higher than those in NC group (P>0.05); 2) Taxonomic annotation showed that Bacteroidetes and Firmicutes accounted for the highest proportion, whereas no significant differences in relative abundance of the two bacteria phyla were observed among the experimental groups and the NC group (P>0.05). The relative abundance of Ruminococcus and Lactobacillus were significantly higher in the experimental groups than those in the NC group (P<0.05). In summary, under the conditions of this experiment, increasing the amount of exercise can increase the abundance and diversity of rumen microorganisms of Tan sheep, change the relative abundance of some bacteria, but have no significant impact on the uniformity of rumen microorganisms.

Key words: Tan sheep, exercise, metagenomics, rumen, microflora

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