饲粮添加赖氨酸对肉牛粪便发酵参数和微生物菌群结构的影响

doi:10.11843/j.issn.0366-6964.2024.05.022

摘要/Abstract

摘要： 本试验旨在探究饲粮中添加赖氨酸对肉牛粪便发酵参数、微生物多样性和菌群结构的影响。选取4头健康、体重相近的锦江牛，随机分为2组，每组2头，采用有重复的2×2拉丁方设计，对照组饲喂基础饲粮，处理组饲喂基础饲粮+0.20%赖氨酸。试验期30 d，分2期进行，每期前10 d为适应期，后5 d为采样期。采集粪便样品进行发酵参数和微生物多样性及菌群结构的检测分析。结果显示：1)饲粮添加赖氨酸显著提高了粪便中异丁酸、异戊酸和支链挥发性脂肪酸的含量(P<0.05)；2)饲粮添加赖氨酸对粪便微生物的丰富度和均匀度均没有显著影响(P>0.05)；3)物种注释发现，饲粮添加赖氨酸显著提高了粪便中毛螺菌科NK4A136群(Lachnospiraceae NK4A136 group)的相对丰度，降低了克里斯滕森菌科R-7群(Christensenellaceae R-7 group)的相对丰度(P<0.05)；4)主坐标分析(PCoA)和相似性分析(ANOSIM)均显示，添加赖氨酸对粪便微生物群落结构无显著影响。综上，饲粮中添加0.20%赖氨酸可以提高锦江牛粪便中异丁酸、异戊酸和支链挥发性脂肪酸的含量，同时改变毛螺菌科NK4A136群和克里斯滕森菌科R-7群的相对丰度，但对微生物多样性和群落结构无显著影响。

关键词: 赖氨酸, 粪便, 发酵参数, 微生物多样性, 菌群结构

Abstract: This study was conducted to investigate the effects of dietary lysine supplementation on fecal fermentation parameters, microbial diversity and microflora structure of beef cattle. Four healthy Jinjiang cattle with similar body weight were randomly divided into two groups with two cattle in each group. A repeated 2×2 Latin square design was adopted with the control group fed the basal diet and the treatment group fed the basal diet +0.20% of lysine. The experiment lasted for 30 days and was divided into 2 phases, in which the first 10 days of each phase designated as the adaptation period and the last 5 days being the sample collection period. Fecal samples were collected and then analyzed for fermentation parameters, microbial diversity and microflora structure. The results showed as follows: 1) Dietary supplementation of lysine significantly increased the concentrations of isobutyrate, isovalerate and branched-chain volatile fatty acids in feces (P<0.05); 2) Lysine supplementation had no significant effect on fecal microbial richness and evenness (P>0.05); 3) Taxonomic annotation found that the supplementation of lysine significantly increased the relative abundance of Lachnospiraceae NK4A136 group in feces, whilst the relative abundance of Christensenellaceae R-7 group was decreased as compared to the control group (P<0.05); 4) Both principal coordinate analysis (PCoA) and analysis of similarity (ANOSIM) showed that lysine supplementation had no significant effect on fecal microbial community structure. In conclusion, dietary supplementation of 0.20% lysine could improve the concentrations of isobutyrate, isovalerate and branched-chain volatile fatty acids in feces, as well as alterations in the relative abundances of Lachnospiraceae NK4A136 group and Christensenellaceae R-7 group, but has no significant effect on fecal microbial diversity and microflora structure.

Key words: lysine, feces, fermentation parameter, microbial diversity, microflora structure

中图分类号:

S823.5

龙唐晖, 詹彦波, 廖观香, 陈新锋, 张健, 李艳娇, 欧阳克蕙, 邱清华. 饲粮添加赖氨酸对肉牛粪便发酵参数和微生物菌群结构的影响[J]. 畜牧兽医学报, 2024, 55(5): 2042-2049.

LONG Tanghui, ZHAN Yanbo, LIAO Guanxiang, CHEN Xinfeng, ZHANG Jian, LI Yanjiao, OUYANG Kehui, QIU Qinghua. Effects of Dietary Lysine Supplementation on Fecal Fermentation Parameters and Microbial Flora Structure of Beef Cattle[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2042-2049.

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