助产对奶牛产后子宫菌群的影响

doi:10.11843/j.issn.0366-6964.2022.12.035

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (12): 4501-4506.doi: 10.11843/j.issn.0366-6964.2022.12.035

助产对奶牛产后子宫菌群的影响

宁心暖, 李洁, 房辉, 余思源, 梁家禧, 张璐, 靳亚平^*, 周栋^*

西北农林科技大学动物医学院农业部动物生物技术重点实验室, 杨凌 712100

收稿日期:2022-07-28 出版日期:2022-12-23 发布日期:2022-12-25
通讯作者: 靳亚平，主要从事动物生殖内分泌与繁殖障碍性疾病研究，E-mail：yapingjin@163.com；周栋，主要从事动物生殖内分泌、生殖免疫、微生物与免疫研究，E-mail：zhoudong1949@nwafu.edu.cn
作者简介:宁心暖(1997-)，女，河南周口人，硕士生，主要从事家畜生殖调控研究，E-mail：ningxn6688@163.com；李洁(1997-)，女，甘肃定西人，硕士生，主要从事家畜生殖调控研究，E-mail：lijie976018@163.com。宁心暖和李洁为同等贡献作者
基金资助:
国家自然科学基金(31702310)

Effect of Assisted Calving on Postpartum Uterine Flora of Dairy Cow

NING Xinnuan, LI Jie, FANG Hui, YU Siyuan, LIANG Jiaxi, ZHANG Lu, JIN Yaping^*, ZHOU Dong^*

Key Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China

Received:2022-07-28 Online:2022-12-23 Published:2022-12-25

摘要/Abstract

摘要： 在奶牛养殖业中，难产是奶牛临床上的常发病，助产是处理难产的常用手段。助产的发生会导致母牛受胎率降低、冻精使用量增加、空怀时间增加和首次配种时间后移等繁殖问题。本研究从细菌学角度分析助产对奶牛产后子宫菌群的影响，以期为提高牧场受胎率提供数据参考。在宁夏地区某大型奶牛场，以助产和非助产奶牛为研究对象，在产后45 d进行子宫灌洗，采集灌洗液样本共7份。通过细菌分离培养、16S rRNA测序等方法分离细菌。结果共分离出10个细菌属，12种细菌，其中，非助产奶牛子宫冲洗液中分离出链霉菌属、链球菌属、肠杆菌属、葡萄球菌属、气球菌属5个属的细菌；助产奶牛子宫冲洗液分离出志贺菌属、不动杆菌属、假单胞菌属、棒状杆菌属、芽胞杆菌属、气球菌属、肠杆菌属、葡萄球菌属、链球菌属等9个属的细菌。本研究的结果表明，助产和非助产奶牛子宫菌群差异显著，助产导致子宫内菌群多样性增加。

关键词: 奶牛, 助产, 子宫灌洗液, 菌群

Abstract: Dystocia is a common clinical disease of dairy cows, and assisted calving is a common method to deal with it. The assisted calving can lead to the reduction of conception rate, the increase of frozen sperm use, the increase of empty pregnancy days and the delay of the first mating time. This study aims to analyze the effect of assisted calving on postpartum uterine microbiota of dairy cow, and to provide data reference for improving the conception rate in pasture. In a large dairy farm in Ningxia, a total of 7 samples of lavage fluid were collected from both unassisted and assisted calving cows at 45 days after delivery. Bacteria were isolated, cultured, and sequenced by 16S rRNA sequencing. A total of 10 bacterial genera and 12 kinds of bacteria were isolated, among which 5 genera of bacteria including Streptomyces, Streptococcus, Enterobacter, Staphylococcus and Aerococcus were isolated from the uterine washing fluid of non-assisted calving cows. Nine genera of bacteria such as Shigella, Acinetobacter, Pseudomonas, Corynebacterium, Bacillus, Aerococcus, Enterobacter, Staphylococcus and Streptococcus were isolated from the uterine washing fluid of assisted calving cows. Our results showed that the uterine microbiota of assisted calving and non-assisted calving cows were significantly different. Assisted calving resulted in increased diversity of uterine microflora in cows.

Key words: cow, assisted calving, uterine lavage fluid, microbiome

中图分类号:

S857.2

宁心暖, 李洁, 房辉, 余思源, 梁家禧, 张璐, 靳亚平, 周栋. 助产对奶牛产后子宫菌群的影响[J]. 畜牧兽医学报, 2022, 53(12): 4501-4506.

NING Xinnuan, LI Jie, FANG Hui, YU Siyuan, LIANG Jiaxi, ZHANG Lu, JIN Yaping, ZHOU Dong. Effect of Assisted Calving on Postpartum Uterine Flora of Dairy Cow[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4501-4506.

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助产对奶牛产后子宫菌群的影响

Effect of Assisted Calving on Postpartum Uterine Flora of Dairy Cow

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