基于转录组测序筛选鼠伤寒沙门菌hfq基因缺失菌的差异表达基因

doi:10.11843/j.issn.0366-6964.2019.12.023

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (12): 2578-2584.doi: 10.11843/j.issn.0366-6964.2019.12.023

• 研究简报 • 上一篇

基于转录组测序筛选鼠伤寒沙门菌hfq基因缺失菌的差异表达基因

杨阳¹, 杨琦^1,2,3*, 董然然^1,2, 潘永¹, 李晨⁴, 刘丽娟⁵, 文明^1,2,3, 周碧君^1,2,3, 王开功^1,2,3

1. 贵州大学动物科学学院, 贵阳 550025;
2. 贵州大学动物疫病研究所, 贵阳 550025;
3. 贵州省动物疫病研究室, 贵阳 550025;
4. 贵州省畜禽资源遗传管理站, 贵阳 550025;
5. 贵州都匀市动物疫病预防控制中心, 都匀 558000

收稿日期:2019-06-27 出版日期:2019-12-23 发布日期:2019-12-20
通讯作者: 杨琦,主要从事预防兽医学的教学和科研工作,E-mail:as.qyang@gzu.edu.cn
作者简介:杨阳(1996-),贵州普定人,硕士生,主要从事动物微生物研究,E-mail:782972566@qq.com
基金资助:
国家自然科学基金（31602065）；贵州省科学技术基金（科合LH字[2017]7263；黔科合平台人才[2017]5788；黔科合基础黔科合基础[2016]1047）；贵州大学博士基金项目（贵大人基合字（2015）61号）

Screening of Differentially Expressed Genes of Salmonella typhimurium hfq Gene Deletion Bacteria Based on Transcriptome Sequencing

YANG Yang¹, YANG Qi^1,2,3*, DONG Ranran^1,2, PAN Yong¹, LI Chen⁴, LIU Lijuan⁵, WEN Ming^1,2,3, ZHOU Bijun^1,2,3, WANG Kaigong^1,2,3

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Institute of Animal Diseases, Guizhou University, Guiyang 550025, China;
3. Guizhou Animal Disease Laboratory, Guiyang 550025, China;
4. Guizhou Province Livestock and Poultry Resource Genetic Management Station, Guiyang 550025, China;
5. Animal Disease Prevention and Control Center of Duyun City, Duyun 558000, China

Received:2019-06-27 Online:2019-12-23 Published:2019-12-20

摘要/Abstract

摘要： 旨在鼠伤寒沙门菌hfq基因缺失株转录组测序中分析适应环境变化及细菌分泌通路，筛选相关基因。通过使用实时荧光定量PCR对鼠伤寒沙门菌hfq基因缺失株转录组测序结果进行验证，从而对鼠伤寒沙门菌hfq基因缺失株的细菌趋化性通路、细菌双组分通路、细菌分泌通路进行深入分析，筛选相关的重要调控基因。结果显示：在细菌趋化性通路中筛选出yiaD、STM3138、STM3216和malE等4个共表达基因；在细菌分泌通路中筛选出spaP、invA、prgH、invE、spaS、invG和ssaV等7个共表达基因；在细菌双组分通路中筛选出ybfM、htrA、pagO、STM3138、STM3031、ttrB、hilD、pstS、STM1530、hilA、pgtC、hydH、pocR、STM3216、ttrR和fljB等16个共表达基因。在鼠伤寒沙门菌hfq基因缺失株的趋化性通路、细菌双组分通路、细菌分泌通路中筛选出差异表达基因，hfq能够对这些基因进行调控，从而影响如运动性、毒力等相关作用，为沙门菌中的sRNA与hfq后续研究及沙门菌的防治奠定一定基础。

关键词: 沙门菌, 沙门菌hfq基因缺失株, sRNA伴侣蛋白Hfq

Abstract: This study aimed to analyze the adaptation to environmental changes and bacterial secretion pathways in transcriptome sequencing of hfq gene deletion strains of Salmonella typhimurium and screen related genes. Real-time fluorescence quantitative PCR was used to verify the transcriptome sequencing results of hfq gene deletion strains of S. typhimurium, so as to conduct in-depth analysis on the bacterial chemotactic pathways, bicomponent pathways and secretion pathways of the hfq gene deleted strain of S. typhimurium, and screen the relevant important regulatory genes. Results were as follows:Four co-expressed genes, including yiaD, STM3138, STM3216 and malE, were screened out in the bacterial chemotaxis pathways. Seven co-expressed genes, including spaP, invA, prgH, invE, spaS, invG and ssaV, were screened from the bacterial secretion pathways. Sixteen co-expressed genes, including ybfM, htrA, pagO, STM3138, STm3031, ttrB, hilD, pstS, STM1530, hilA, pgtC, hydH, pocR, STM3216, ttrR and fljB, were screened in the bacterial two-component pathways. Differentially expressed genes were screened out from bacterial chemotactic pathways,two-component pathways and secretion pathways in hfg gene deleted S.typhimurium strain.And hfg could regulate these genes to affect related functions such as motility and virulence. The results laid a certain foundation for the prevention and cure of Salmonella and further study of sRNA and hfg in Salmonella.

Key words: Salmonella, hfq deleted strain, sRNA chaperone Hfq

中图分类号:

杨阳, 杨琦, 董然然, 潘永, 李晨, 刘丽娟, 文明, 周碧君, 王开功. 基于转录组测序筛选鼠伤寒沙门菌hfq基因缺失菌的差异表达基因[J]. 畜牧兽医学报, 2019, 50(12): 2578-2584.

YANG Yang, YANG Qi, DONG Ranran, PAN Yong, LI Chen, LIU Lijuan, WEN Ming, ZHOU Bijun, WANG Kaigong. Screening of Differentially Expressed Genes of Salmonella typhimurium hfq Gene Deletion Bacteria Based on Transcriptome Sequencing[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2578-2584.

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基于转录组测序筛选鼠伤寒沙门菌hfq基因缺失菌的差异表达基因

Screening of Differentially Expressed Genes of Salmonella typhimurium hfq Gene Deletion Bacteria Based on Transcriptome Sequencing

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