一株沙门菌温和噬菌体PEA2-3生物学特性及基因组分析

doi:10.11843/j.issn.0366-6964.2023.04.027

摘要/Abstract

摘要： 泛耐药菌和多重耐药菌的检出率不断上升，噬菌体在防控治疗耐药菌的传播和感染上有良好的应用前景。本研究旨在利用耐药菌株分离出宽裂解谱温和噬菌体，丰富了沙门菌温和噬菌体库。利用丝裂霉素C诱导、分离纯化出1株温和性噬菌体，命名为Salmonella virus PEA2-3，测定其部分生物学特性并进行生物信息学分析。透射电镜观察显示，噬菌体头部宽约为61 nm，尾长93 nm，属于有尾噬菌体目（Caudovirales）、罗斯蒙特病毒属（Rosemountvirus）；最佳感染复数为0.01；一步生长曲线显示潜伏期为20 min，裂解期约为80 min；能够裂解不同来源的沙门菌（38/72）及大肠杆菌（23/52），是1株宽裂解谱的溶原性噬菌体，具有较好的应用价值。基因组测序结果显示，该噬菌体基因组全长52 770 bp，GC含量为45.99%，基因组共注释到73个CDS，8个CDS被赋予已知功能蛋白，不存在抗生素抗性基因或者毒力基因。噬菌体PEA2-3全基因组提交至NCBI的GenBank数据库，获得序列号为MW508890。结果显示，这是1株能同时裂解大肠杆菌与沙门菌的宽裂解谱高效价温和噬菌体，为防控和治疗耐药菌传播提供了良好的应用基础。

关键词: 温和噬菌体, 沙门菌, 生物学特性, 基因组分析

Abstract: The detection rates of Pan drug resistant bacteria and multi drug resistant bacteria are increasing, and phages have good application prospects in preventing the transmission and infection of treatment resistant bacteria. The purpose of this study was to isolate mild bacteriophages with wide cleavage spectrum and enrich the Salmonella mild phage library. A temperate phage named Salmonella virus PEA2-3 was isolated and purified using mitomycin C induction, and some of its biological characteristics were determined and bioinformatics analysis was performed. TEM revealed that the phage head was approximately 61 nm wide and the tail was 93 nm long, and it belonged to the order Caudovirales, genus Rosemountvirus. The optimal multiplicity of infection was 0.01; The one-step growth curve showed an incubation period of 20 min and a lysis period of approximately 80 min; And was able to lyse Salmonella (38/72) and Escherichia coli (23/52) of different origins, which indicated it to be a lysogenic phage with a wide lysis spectrum, that had better application value. The genome sequencing results showed that its genome was 52 770 bp in length with a GC content of 45.99%, and the genome was annotated to a total of 73 CDS, 8 CDS of which were assigned to known functional proteins, and no antibiotic resistance genes or virulence genes was existed. The whole genome of phage PEA2-3 was submitted to the GenBank database of NCBI and obtained the sequence number of MW508890. The results suggest that this strain is a highly efficient and mild phage with a wide lytic spectrum that can lyse Escherichia coli and Salmonella simultaneously, providing a good application basis for the prevention and control of the spread of drug-resistant bacteria.

Key words: temperate phage, Salmonella, biological properties, genomic analysis

中图分类号:

Q939.48

李德豪, 李宜穗, 马静云, 孙媛, 张祥斌. 一株沙门菌温和噬菌体PEA2-3生物学特性及基因组分析[J]. 畜牧兽医学报, 2023, 54(4): 1632-1640.

LI Dehao, LI Yisui, MA Jingyun, SUN Yuan, ZHANG Xiangbin. Biological Characteristics and Genome Analysis of Lysogenic Salmonella Phage PEA2-3[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1632-1640.

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