关键词: 鸡, 骨髓源树突状细胞, 鉴定, 生物学特征

Abstract: In order to develop the method of induction and cultivation chicken bone marrow-derived dendritic cells (BMDCs) in vitro, and analyze the main characteristics of DCs,the bone marrow precursors were collected by differential adherence method after separating the chicken bone marrow cells using Lymphoprep. Then induction and differentiation of the bone marrow precursors were conducted by using granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4). On day 7 of culture, the cells were stimulated with lipopolysaccharide (LPS) for 48 h, then the DCs were identified by morphologic and phenotypic characteristics using inverted phase-contrast microscope, scanning electron microscope and flow cytometry. The results showed that the cells volume enlarged, and the surface of the cells were dendritically shaped, phenotypic analyses of non-stimulated cells showed high expression of MHC class Ⅱ and CD11c. After stimulating by LPS, the veils became larger, these cells appeared a typical morphological feature of BMDCs under electron microscope, and the expression of MHC class Ⅱ was significantly increased. These data indicated that we succesfully obtained a large quantity of BMDCs with higher purity according to this method. This study laid a foundation for further research on the biological function of BMDCs in poultry and the role of BMDCs in the occurrence of certain diseases.

Key words: chicken, bone marrow-derived dendritic cells, identification, biological characteristics