两纯系鸡胃肠道黏膜细菌l6S rDNA的PCR-DGGE分析比较

Abstract

Abstract:

The experiment were conducted to analyze and compare the diversity and dynamic change of the gastrointestinal mucosa-associated microbiota (MAM) of high quality chicken line A (Pure Line♂, Group A) and Huiyang Beard chicken (Pure Line♂, Group B) during day 1-28. The V3 region of 16S rDNA of the mucosa-associated microbiota of two chicken pure lines were analyzed by Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) at day 1, 3, 7, 14, 21 and 28, respectively. DGGE profiles, based on the number of bands of the gel, indicated the MAM of ileum between the two groups were significantly different at day 28. The similarity of the MAM in craw, glandular stomach and cecum between the two groups were higher than 70%, while the similarity in duodenum, jejunum and ileum were 40%, 44% and 18% at day 28, respectively. The alterations of MAM of ileum were remarkably different between the two groups during day 21-28, with 70% change in group A and 11% change in group B. This diversity between the high quality chicken line A and Huiyang Beard chicken might be influenced by the host genetic and, at the same time, might be one of the possible cause for their different growth performance.

CLC Number:

S831.2

ZHANG Jie, RONG Ting, LIU Zhi-chang, WANG Gang,LIN Hai-dan,BEI Jin-long,CHEN Zhuang. Comparative Analysis of 16S rDNA of the Gastrointestinal Mucosa-associated Microbiota between Two Pure Lines Chicken by PCR-DGGE[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Comparative Analysis of 16S rDNA of the Gastrointestinal Mucosa-associated Microbiota between Two Pure Lines Chicken by PCR-DGGE

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