鸭CD8α基因克隆及不同禽类分子进化分析

Abstract

Abstract:

The coding sequence of CD8α was cloned by RT-PCR and LD-PCR in five duck populations (Jinding Duck, Cherry Valley duck, muscovy, mallard and spot-billed duck). The 9 sequences （another 4 sequences from GenBank） were analyzed by online software of bioinformatics(the best nucleotide substitution model) was selected by Jmodeltest, the nucleotide substitution saturation was assessed with DAMBE, and the selective pressure on amino acid sites was tested by PAML. The results of sequencing showed the coding sequence of CD8α was conservative except a few point mutations. The model “GTR+G” was selected as the best nucleotide substitution model, the sequences were experienced full substitution unsaturation. The phylogenetic trees of CD8α among different fowl were generated by MEGA 5.0, and the 9 fowl were divided into 3 parts according to CD8α sequences. The 7 amino acid sites undergoing positive selection were detected, 4 of them were in the important function regions by the site-specific model. In summary, the coding sequence of CD8α was cloned successfully，and it was conservative in molecular evolution, which may be helpful for studying evolution, structure and function of CD8α among fowl.

CLC Number:

S852.4

XU Qi, HUANG Zheng-yang, SUN Zhi-ming, CHEN Yang, ZHAO Wen-ming, ZHANG Yang，LI Xiu, DUAN Xiu-jun, CHEN Gu-hong. Molecular Cloning and Evolution of CD8α Gene in Duck Populations[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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References

［1］JANEWAY C A. The T cell receptor as amulticomponent signalling machine: CD4/CD8 coreceptors and CD45 in the T cell activation ［J］. Annu Rev Immunol, 1992, 10: 645-674.

［2］杜建, 邵爱华, 朱江. 暗纹东方鲀CD8α全长cDNA的克隆及序列分析［J］. 江苏农业科学, 2009,(5): 23-27.

［3］KOTHLOW S, MANNES N K, SCHAERER B, et al. Characterization of duck leucocytes by monoclonal antibodies［J］. Dev Comp Immunol, 2005, 29(5): 733-748.

［4］袁舟. 稳定表达鸡CD4、CD8α细胞系的建立与鉴定［D］. 扬州: 扬州大学，2008.

［5］PATEL S, OVERGRD A C, NERLAND A H. CD8a and CD8b in Atlantic halibut, Hippoglossus hippoglossus: Cloning, characterization and gene expression during viral and bacterial infection［J］. Fish Shellfish Immunol, 2008, 25: 570-580.

［6］SAWYER S L, WU L I, EMERMAN M, et al. Positive selection of primate TRIM5α identifies a critical species-specific retroviral restriction domain［J］. Proc Natl Acad Sci U S A, 2005, 102:2832-2837.

［7］龚远英, 彭旻晟, 周伟平, 等. 哺乳动物cd59基因的进化［J］. 中国科学C辑(生命科学), 2007, 37(5): 544-550.

［8］VERGRD A C, FIKSDAL I U, NERLAND A H, et al. Expression of T-cell markers during Atlantic halibut (Hippoglossus hippoglossus L.)ontogenesis［J］. Dev Comp Immunol, 2011, 35: 203-213.

［9］VOIGHT B F, KUDARAVALLI S, WEN X, et al. A map of recent positive selection in the human genome［J］. PLoS Biol, 2006, 4: 72.

［10］WANG E T, KODAMA G, BALDI P, et al. Global landscape of recent inferred Darwinian selection for Homo sapiens［J］. Proc Natl Acad Sci USA, 2006, 103: 135-140.

［11］CHIDA A S, GOYOS A, ROBERT J. Phylogenetic and developmental study of CD4, CD8αβ and β T cell co-receptor homologs in two amphibian species, Xenopus tropicalis and Xenopus laevis［J］. Dev Comp Immunol, 2011, 35: 366-377.

［12］李卫, 白家媛, 许媛媛, 等. 麻鸭和樱桃谷北京鸭的CD3ε、CD4、CD8α基因ORF克隆及序列分析［J］. 中国农业科学, 2009, 42(12): 4428-4434.

［13］GAO G F, TORMO J, GERTH U C，et a1．Crystal structure of the complex between human CD8α and HLA-A2［J］．Nature, 1997, 387: 630-634.

［14］TANABE M, KARAKI S, TAKIGUCHI M，et a1．Antigen recognition by the T-cell receptor is enhanced by CD8 alpha-chain binding to the alpha 3 domain of MHC class I molecules，not by signaling via the cytoplasmic domain of CD8 alpha［J］．Int Immunol, 1992, 4: 147-152.

［15］徐胜威. 斜带石斑鱼CD8α和CD8β基因克隆及表达模式分析［D］. 广东：中山大学，2010.

［16］SHAW A S, CHALUPNY J, WHITNEY J A，et a1．Short related sequences in the cytoplasmic domains of CD4 and CD8 mediate binding to the amino-terminal domain of the p56lck tyrosine protein kinase［J］．Mol Cell Biol, 1990, 10: 1853-1862.

［17］HOU Z C, XU G Y, SU Z, et al. Purifying selection and positive selection on the myxovirus resistance gene in mammals and chickens［J］. Gene, 2007, 396: 188-195.

［18］柳晓磊, 翁群芳, 胡美英, 等. 昆虫化学感受蛋白基因的进化分析［J］. 华中农业大学学报, 2011, 30(2): 177-181.

［19］YANG Z. Inference of selection from multiple species alignments［J］. Curr Opin Genet Dev, 2002, 12: 688-694.

［20］YANG Z, BIELAWSKI J P. Statistical methods for detecting molecular adaptation［J］.Trends Ecol Evol, 2000,15(12): 496-503.

［21］KHAN M M, RYDN A M, CHOWDHURY M S, et al. Maximum likelihood analysis of mammalian p53 indicates the presence of positively selected sites and higher tumorigenic mutations in purifying sites［J］. Gene, 2011, 483:29-35.

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Molecular Cloning and Evolution of CD8α Gene in Duck Populations

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