TLR-4介导的鹅β-防御素1抗肠炎沙门菌感染的信号传导机制初探

Abstract

Abstract:

The objective of this study was to study the biological characteristics of goose AvBD1 and preliminary attempts to explore its resistance to Salmonella enteritidis mechanism. The mRNA of goose AvBD1 was cloned from bone marrow of the gooses by RT-PCR. The cDNA of goose AvBD1 was sub-cloned into EcoRⅠ and SalⅠ sites of pGEX-6p-1 vector to construct recombinant plasmid pGEX-goose AvBD1. The recombinant plasmid was transformed into E. coli BL21 and the bacteria was induced with IPTG. It was demonstrated by SDS-PAGE that a 31 kDa protein which was equal to goose AvBD1 protein in molecular weight was highly expressed. The sequence analysis showed that the gene fragment of AvBD1 contained 198 bp, and encoded 65 amino acids. Homology analysis showed that goose AvBD1 shared the highest percentage of amino acid homology (77.1%) with ostrich AvBD1.The purified recombinant goose AvBD1 exhibited extensive antimicrobial activity against twelve investigated bacteria strains, including Gram-positive and Gram-negative. At high salt ions conditions, antimicrobial activity of recombinant goose AvBD1 protein against both Staphylococcus aureus and Proteus mirabilis decreased significantly and hemolysis activity of the recombinant protein was extremely low. Experimental results show that Salmonella enteritidis did induced goose AvBD1 gene expression in bone marrow, at the same time goose AvBD1 resistance to Salmonella enteritidis infection induces expression of TLR4 may be mediated signal transduction.

CLC Number:

S852.4

ZHANG Ming-yue, ZHANG Ke-xin, XIN Sheng-nan, HAN Zong-xi, SHAO Yu-hao, LIU Xiao-li, LIU Sheng-wang, MA De-ying. Preliminary Exploration of the Signal Transduction Mechanisms of the Goose Beta Defensin 1 Resistance to Salmonella Enteritidis Infection Mediated by Toll-like Receptor 4[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: .

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Preliminary Exploration of the Signal Transduction Mechanisms of the Goose Beta Defensin 1 Resistance to Salmonella Enteritidis Infection Mediated by Toll-like Receptor 4

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